Proceeding2562

1537 การประชุมวิชาการระดับชาติมหาวิทยาลัยทักษิณ ครั้งที่ 29 ประจ�ำปี 2562 วิจัยและนวัตกรรมเพื่อการพัฒนาที่ยั่งยืน cell better than that of EDTA. This might explain the observation that DNA concentration extracted from CPDA1 was statistically significant higher than the DNA extracted from EDTA blood after venipuncture and store for 30 days. Heparin is least effective on cell preservation, since hemolysis can be observed within 9 days in refrigerator and inhibits PCR amplification (14). In this study, both EDTA and CPDA1 were not interfered with DNA amplification in PCR. These results suggested that CPDA1 is alternative and comparable anticoagulants to be used in blood bank for both serological and molecular studies. Acknowledgement This project was supported by Faculty of Medical Technology, Prince of Songkla University and thankful for blood donors from Regional Blood Centre XII Songkhla, Thailand. References [1] Dauber EM. (2018). “Somatic mosaicisms of chromosome 1 at two different stages of ontogenetic development detected by Rh blood group discrepancies”, Haematologica . 103, 1-11. [2] Kim B. (2018). “Application of Multiplex Ligation-Dependent Probe Amplification Assay for Genotyping Major Blood Group Systems Including DEL Variants in the D-Negative Korean Population”, Annals of laboratory medicine . 32, 32-8. [3] Intharanut K. (2017). “Red Cell Genotyping by Multiplex PCR Identifies Antigen-Matched Blood Units for Transfusion Dependent Thai Patients”, Transfus Med Hemother . 44, 358–64. [4] Mohammadi M. (2017). “Relationship between human leukocyte antigen (HLA)-DQA1*0102/HLA- DQB1*0602 polymorphism and preeclampsia”, Int J Reprod Biomed (Yazd), 15, 569-74. [5] Kjaer KM. (2002). “Human platelet antigen 1 (HPA 1) genotyping with 5' nuclease assay and sequence-specific primers reveals a single nucleotide deletion in intron 2 of the HPA 1a allele of platelet glycoprotein IIIa”, Br J Haematol. 117, 405-8. [6] Youssoufi F. (2017). “ The prevalence of human leukocyte antigen-B*57:01 allele in HIV-1-infected Moroccan subjects”, Gene Rep . 9, 108–10. [7] Tomee Y. (2009). “Detection of Mia genotype of DNA extracted from blood and hair roots by Polymerase Chain Reaction”, Bull Chiang Mai Assoc Med Sci . 42, 242-50. [8] Dhage H. Top 5 Anticoagulants Used in Hematology Laboratory. [cited 2018 14 November]. Available from: http://www.biologydiscussion.com/hematology-2/laboratory-hematology-2/top-5- anticoagulants-used-in-hematology-laboratory-biology/80371. [9] Vijay Shankar S. (2017). Anticoagulants in Hematologic Investigations [cited 2018 14 November]. Available from: http://ilovepathology.com/anticoagulants-in-hematologic-investigations/ [10] Holodniy M. (1991). “Inhibition of human immunodeficiency virus gene amplification by heparin”, J Clin Microbiol. 29, 676-9. [11] Izraeli S. (1991). “Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood”, Nucleic Acids Res. 19, 6051.