Proceeding2562

1570 การประชุมวิชาการระดับชาติมหาวิทยาลัยทักษิณ ครั้งที่ 29 ประจ�ำปี 2562 วิจัยและนวัตกรรมเพื่อการพัฒนาที่ยั่งยืน Results of anti-p16 INK4a antibody from serum (n=97) are correlated with previous Western blot results (5). The absorbance (OD 450 ) of positive and negative serum samples were significantly difference (p<0.001, figure 1). The data of sensitivity, specificity of ELISA were 94.0%, 82.97%, respectively compared to previous Western blot result (Table 1.). From all 97 serum, 3 serum showed false negative results (3.09%) from lower than cut-off value and 8 from 97 (8.25%) serum gave false positive results. Conclusions This anti-p16 INK4a antibody ELISA condition gave high sensitivity and specificity when tested with positive and negative serum. However, this ELISA condition need to further optimize to increase sensitivity, specificity and used as screening test for abnormal cervical lesion in HPV infected women. Acknowledgements Research grant and research workplace were supported by Faculty of Medical Technology, Prince of Songkhla University. References [1] Paaso A. (2016) “Effect of Human papillomavirus-specific immunity on the outcome of HPV infections in women”, University of Turku: Painosalama Oy-Turku. [2] Lukas J (1995) “Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16”, Nature. 1995; 375. [3] Reuschenbach M. (2008) “Characterization of humoral immune responses against p16, p53, HPV16 E6 and HPV16 E7 in patients with HPV ‐ associated cancers”, International Journal of Cancer. 123(11): 2626-31. [4] Rosales R. (2001) “Antibodies against human papillomavirus (HPV) type 16 and 18 E2, E6 and E7 proteins in sera: correlation with presence of papillomavirus DNA”, J Med Virol. 65(4): 736-44. [5] Chuerduangphui J. (2018) “Association of antibody to E2 protein of human papillomavirus and p16INK4A with progression of HPV-infected cervical lesions” Medical Oncology. 93. [6] Chao Y. (2011) “Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy”, Applied Microbiology and Biotechnology. 381.