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al., 2009) —o ª¥ o°¤¼¨—´ Š„¨nµªÁºÊ°Š˜oœ‹¹Š¤¸Âœª‡· —š¸É‹³œÎµÂ¨„Á‡ (×¥ž¦µ«‹µ„„µ¦Äo mediator ¦nª¤„´Â¨„Á‡)
¤µž¦³¥»„˜rčo ¦nª¤„´ÁŽ¨¨¼ Á¨Äœ„µ¦„ε‹´—®¤¹ „—oª¥ª·›¸ „µ¦¨°¥¢°Š°µ„µ«°°„‹µ„„¦³—µ¬®œ´Šº°¡·¤¡r Á„nµ Ž¹ÉŠ
Šµœª·‹´¥œ¸Ê‹³šÎµ„µ¦Ÿ¨· ˜Á°œÅŽ¤r—oª¥ª·›¸„µ¦šµŠ¸ª£µ¡ ×¥Ÿ¨·˜ÁŽ¨¨¼Á¨‹µ„ÁºÊ°¦µœ·— Aspergillus niger ¨³Ÿ¨·˜
¨„Á‡‹µ„ÁºÊ°¦µÁœnµ µª (white rot fungus) œ·— Pycnoporus sanguineus µ¥¡´œ›»rš¸É‡´—Â¥„Å—oĜž¦³Áš«Åš¥
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œµ—ž¦³¤µ– 1 œ·Ê ª x 1 œ·Êª ¨³ÂnœÊεš·Ê ŠÅªo 3 ´É ªÃ¤Š Á¡ºÉ°Ä®o„¦³—µ¬°n°œ˜´ªÂ¨³Šnµ¥˜n°„µ¦˜¸ „¦³‹µ¥Ä®oÁž}œÁ¥ºÉ°
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1 „µ¦Ÿ¨·˜ÁŽ¨¨¼Á¨‹µ„ÁºÊ°¦µ Aspergillus niger ¨³ª·Á‡¦µ³®rž¦·¤µ–°‡˜·ª·˜¸ (activity) °ŠÁ°œÅŽ¤r
1.1 Á˜¦¸¥¤°µ®µ¦Á¨¸Ê¥ŠÁºÊ°¼˜¦ PDA (potato dextrose agar) ¨³¼˜¦ production medium ¨oªšÎ µ„µ¦
Á¡µ³ÁºÊ °¦µ A. niger ×¥™nµ¥ÁºÊ°š¸ÉÁ¨¸Ê¥ŠÅªo Ĝ°µ®µ¦ª»oœÂ ȊÁ°¸¥Š ¨ŠÅžÄœ‹µœÁ¡µ³ÁºÊ ° š¸ÉÁš PDA Ūo šÎ µ„µ¦Á¨¸Ê¥Šš¸É
°»–®£¼¤·®o °ŠÁž} œÁª¨µ 7 ª´œ
1.2 Á‹µ³·Êœª»oœš¸É¤¸ÁºÊ °¦µÁ‹¦·°¥¼n ×¥œÎµ·Êœª»oœš¸ÉÁ‹µ³Å—o¤µÁ¨¸Ê ¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°¼˜¦ production
medium ‹Î µœªœ 5 ·Êœ˜n° production medium 100 ¤·¨¨· ¨·˜¦ (1 ·Êœª»oœš¸É¤¸ A. niger ‹³¤¸ÁoœŸn µœ«¼ œ¥r„¨µŠ 1
ÁŽœ˜·Á¤˜¦) Ž¹É ŠÁ˜¦¸¥¤ÅªoĜ ª—¦¼ž¤¡¼nœµ— 250 ¤· ¨¨·¨·˜¦ šÎµ„µ¦n¤Â¨³Ä®o°µ„µ« ×¥Á¨¸Ê¥ŠœÁ‡¦ºÉ°ŠÁ ¥nµš¸É 150
¦°˜n °œµš¸ š¸É °»–®£¼¤·®o °ŠÁž}œÁª¨µ 7 ª´œ ®¨´Š‹µ„œ´ÊœœÎµŸ¨Ÿ¨·˜š¸Éŗo ¤µ„¦°Š—oª¥„¦³—µ¬„¦°Š Whatman Á°¦r 1
×¥Á„ȝÁŒ¡µ³nªœš¸ÉÁž}œ °ŠÁ®¨ªÅªočo ¨³Á„ȝŪoĜ˜¼o Á¥Èœn°ŠÂn Ȋ
1.3 ˜¦ª‹°Â°‡˜·ª·˜¸°ŠÁŽ¨¨¼ Á¨—oª¥ª·›¸ DNS assay ˜µ¤ª·›¸°Š Miller ¨³‡–³ (Miller et al .,
1959)
2 „µ¦Ÿ¨·˜Â¨„Á‡‹µ„ÁºÊ°¦µÁœnµ µªœ·— Pycnoporus sanguineus ¨³ª·Á‡¦µ³®rž¦· ¤µ–°‡˜·ª·˜¸°Š
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2.1 šÎµ„µ¦Á¡µ³Á¨¸Ê¥ŠÁºÊ°¦µ Pycnoporus sanguineus ÁnœÁ—¸ ¥ª„´ÁºÊ°¦µ A. niger
2.2 Á‹µ³·Êœª»oœš¸É¤¸ÁºÊ°¦µÁ‹¦·°¥¼n ×¥œÎµ·Êœª»oœš¸ÉÁ‹µ³Å—o¤µÁ¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°¼˜¦ GYP (glucose
yeast peptone) ‹Îµœªœ 10 ·Êœ˜n° production medium 100 ¤·¨¨· ¨·˜¦ Ž¹ÉŠÁ˜¦¸ ¥¤ÅªoĜ ª—¦¼ž¤¡¼n œµ— 250 ¤·¨¨·¨·˜¦
šÎµ„µ¦n ¤Â¨³Ä®o°µ„µ« ×¥Á¨¸Ê¥ŠœÁ‡¦ºÉ°ŠÁ ¥nµš¸É 150 ¦°˜n°œµš¸ š¸É°»–®£¼¤·®o °ŠÁž}œÁª¨µ 2 ª´ œ
2.3 šÎµ„µ¦Á˜·¤ CuSO4.5H2O ¨ŠÄœ ª—¦¼ž¤¡¼nš¸É n¤Á¨¸Ê¥ŠÁºÊ °°¥¼nª—¨³ 600 ŤÇ¦¨·˜¦ ¨oªšÎ µ„µ¦
Ä®o°µ„µ«œÁ‡¦ºÉ°ŠÁ ¥n µ‡ªµ¤Á¦Èª¦° 150 ¦°˜n°œµš¸˜n° Áž}œÁª¨µ 8 ª´œ ®¨´Š‹µ„œ´ÊœœÎµŸ¨Ÿ¨·˜š¸Éŗo ¤µ„¦°Š—o ª¥
„¦³—µ¬„¦°Š Whatman Á°¦r 1 ×¥Á„ȝÁŒ¡µ³nªœš¸ÉÁž}œ °ŠÁ®¨ªÅªočo ¨³Á„ȝŪoĜ˜¼o Á¥Èœn°ŠÂn Ȋ