เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 305

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥ ¤®µª·š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
Abstract
In Hevea brasiliensis breeding program, total duration for high yielding clone selection consumed over 25
years. Since molecular marker for identification of latex yield potential in early stage will be beneficial for the
breeder. The main objective of this research was to select the gene marker for identification of latex yielding in Hevea
brasiliensis. Gene expression pattern of high and low yielding Hevea clone was analyzed using cDNA-AFLP
technique. Up-regulated transcription-derived fragments (TDFs) were selected, cloned and sequenced. The homology
search of the TDFs against the publicly available genes in the database (GenBank) was performed using the BLAST
sequence alignment program. Eleven candidate genes were obtained; four of them were selected and used to determine
mRNAs levels in latex of four eighteen-months-old high yielding clones and four eighteen-months-old low yielding
clones. The main difference between the high yielding and the low yielding clones was obtained by only TDFLH143
expression pattern. These results indicated that the TDFLH143 gene could possibly be developed as a marker for the
early selection in Hevea breeding program.
Keywords: gene marker, Hevea clone identification, high latex yielding
‡ÎµœÎµ
¥µŠ¡µ¦µÁž} œ¡ºÁ«¦¬“„·‹Îµ‡´œ· —®œ¹ÉŠ °Šž¦³Áš«Åš¥ šÎ µ¦µ¥Å—oÁ oµž¦³Áš«že¨³¤µ„„ªnµ°ŠÂœ¨oµœ
µš ¤¸ ¡ºÊ œš¸Éž¨¼„ž¦³¤µ– 15 ¨oµœÅ¦n (εœ´ „ŠµœÁ«¦¬“„·‹„µ¦Á„¬˜¦, 2552) ˜´ÊŠÂ˜n ¡.«. 2546 ™µ´œª· ‹´¥¥µŠ „¦¤
ª·µ„µ¦Á„¬˜¦Å—o ‹´—„¨»n¤¡´ œ›»r¥µŠnŠÁ¦·¤Ä®oÁ„¬˜¦„¦Á¨º°„ž¨¼ „Áž}œ 3 „¨»n¤ ‡º °„¨»n¤¡´œ›»r¥µŠŸ¨Ÿ¨·˜œÊÎ µ¥µŠ¼Š
„¨»n¤¡´œ›»r ¥µŠŸ¨Ÿ¨·˜œÊε¥µŠÂ¨³ÁœºÊ°Å¤o ¼Š ¨³„¨»n¤¡´œ›»r¥µŠŸ¨Ÿ¨· ˜ÁœºÊ°Å¤o ¼Š ˜n°¥nµŠÅ¦„Șµ¤Á„¬˜¦„¦nªœÄ®n¥´Šœ·¥¤
ž¨¼„„¨»n¤µ¥¡´œ›»rÄ®o Ÿ¨Ÿ¨·˜œÊε¥µŠ¼Š
ަ„¦¤„µ¦ž¦´ž¦» Š¡´œ›»r¥µŠ¡µ¦µÁ¡ºÉ°‡´—Á¨º°„µ¥¡´œ›»rÄ®oŸ¨Ÿ¨· ˜œÊε¥µŠ¼Š ž¦³„°—oª¥ „µ¦Ÿ¤¡´œ›»r
„µ¦ž¨¼„Á¤¨È —Á¡ºÉ°‡´—Á¨º°„¡´œ›»rÁºÊ°Š˜oœ Áž¦¸¥Áš¸¥¡´œ›»r´Êœ˜o œ ¨³„µ¦ž¨¼„Áž¦¸ ¥Áš¸¥¡´œ›»r´Ê œž¨µ¥ ˜o°ŠÄoÁª¨µ
¦ª¤„´œÅ¤nœo°¥„ªnµ 25 že —´Šœ´Ê œ™oµ¤¸Á‡¦ºÉ °Š¤º°‹ÎµÂœ„Ÿ¨Ÿ¨·˜œÊε¥µŠ˜´ÊŠÂ˜nĜ¦³¥³˜o œÇ ‹³¨—¦³¥³Áª¨µ °ŠÃž¦Â„¦¤„µ¦
ž¦´ž¦»Š¡´œ›»rÁ¡ºÉ°‡´—Á¨º°„¡´œ›»r¥µŠÄ®oŸ¨Ÿ¨· ˜¼ŠÅ—o šÎµÄ®o¤¸Ã°„µÅ—o µ¥¡´œ›»rÄ®oŸ¨Ÿ¨·˜œÊε¥µŠ¼Š¦ª—Á¦Èª ¹Ê œ ¦ª¤š´ÊŠ
ž¦³®¥´—Šž¦³¤µ–¨³¡ºÊœš¸É —εÁœ· œ„µ¦°¸„—oª¥ Á‡¦ºÉ°Š®¤µ¥šµŠ¸ªÃ¤Á¨„»¨ ‹¹ŠÁž}œšµŠÁ¨º°„®œ¹ÉŠš¸É ¤¸‡ªµ¤Á®¤µ³¤Äœ
„µ¦ÄoÁž} œÁ‡¦ºÉ°Š¤º°Äœ„µ¦‹ÎµÂœ„Ÿ¨Ÿ¨·˜œÊε¥µŠ °Š¥µŠ¡µ¦µÄœ¦³—´˜oœ„¨o µ
Šµœª· ‹´¥œ¸Êŗo—εÁœ·œ„µ¦ª·Á‡¦µ³®r¨³‡´—Á¨º °„¥¸œš¸É¤¸„µ¦Â—Š°°„°—‡¨o°Š„´Ÿ¨Ÿ¨·˜Äœ¥µŠ¡µ¦µš¸É Ážd —„¦¸—
¨oªž¦³¤µ– 10 že ‹µ„œ´Ê œ‹¹ŠœÎµ¥¸œš¸É ¤¸„µ¦Â—Š°°„°—‡¨o°Š„´Ÿ¨Ÿ¨· ˜Åž‹ÎµÂœ„„µ¦Â—Š°°„ÄœœÊε¥µŠ °Š
¥µŠ¡µ¦µ°µ¥»ž¦³¤µ– 18 Á—º°œ ž¦³„°—oª¥„¨»n ¤Ä®oŸ¨Ÿ¨·˜¼Š 4 µ¥¡´œ›»r¨³„¨»n ¤Ä®oŸ¨Ÿ¨·˜˜Éε 4 µ¥¡´œ›»r Ž¹ÉŠÁž}œ
o °¤¼¨š¸É ε‡´Äœ„µ¦®µ o°¦»žªnµ¥¸œÄ—oµŠ¤¸«´ „¥£µ¡ÄoÁž} œÁ‡¦ºÉ°Š®¤µ¥Äœ„µ¦‡´ —Á¨º°„µ¥¡´œ›»r ¥µŠÄ®oŸ¨Ÿ¨·˜œÊε¥µŠ¼ Š
Ĝ¦³—´˜oœ„¨o µ