เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 306

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Á„ȝœÊÎ µ¥µŠ‹µ„¥µŠ¡µ¦µ„¨»n¤µ¥¡´ œ›»rÄ®oŸ¨Ÿ¨·˜œÊε¥µŠ¼Š (RRIT251 ¨³ RRIM600) ¨³„¨»n¤µ¥¡´ œ›»rÄ®o
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‹µ„œÊε¥µŠ˜µ¤ª·›¸„µ¦š¸É ¡´•œµ ¹ÊœÃ—¥ Áž¨ºÊ°Š (2549) œÎµ total RNA ދεœ„„µ¦Â—Š°°„ °Š¥¸œ—oª¥Áš‡œ·‡ cDNA-
AFLP ž¦³¥»„˜r‹µ„ª·›¸ „µ¦š¸É¦µ¥ŠµœÅªo×¥ Bachem ¨³‡–³ (1998) ¨³ Ko ¨³‡–³ (2003) ‹µ„œ´Ê œ‹¹Š‡´—Á¨º°„™
°Š transcription-derived fragments (TDFs) š¸É ¤¸‡ªµ¤Á o¤¤µ„Äœ„¨»n ¤¥µŠµ¥¡´œ›»rÄ®oŸ¨Ÿ¨·˜œÊε¥µŠ¼Š ª·Á‡¦µ³®r ®µ
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˜¦ª‹°„µ¦Â—Š°°„ °Š¥¸œš¸É ‡´—Á¨º°„—oª¥Áš‡œ·‡ RT-PCR
čo ¨Îµ—´Á °Š¥¸œš¸É ‡´—Á¨º °„Å—o‹µ„Áš‡œ·‡ cDNA-AFLP Áž}œÂÄœ„µ¦´ŠÁ‡¦µ³®rÅ¡¦Á¤°¦rץčo
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Á˜¦¸ ¥¤Âž¨Šš—¨°Šž¨¼ „„¨oµ¥µŠ¡µ¦µµ¥¡´ œ›»r˜nµŠÇ š¸É Ä®oŸ¨Ÿ¨·˜œÊÎ µ¥µŠÂ˜„˜n µŠ„´ œ‹Îµœªœ 8 µ¥¡´œ›»r
ž¦³„°—oª¥µ¥¡´œ›»r Ä®oŸ¨Ÿ¨·˜œÊε¥µŠ¼Š‹Îµœªœ 4 µ¥¡´œ›»rŗo„n RRIT251 (460 „·Ã¨„¦´ ¤/Ŧn/že) BPM24 (340
„· 脦´¤/Ŧn/že) PB235(330 „·Ã¨„¦´¤/Ŧn/že) RRIM600 (310 „·Ã¨„¦´ ¤/Ŧn/že) ¨³Ÿ¨Ÿ¨·˜œÊε¥µŠ˜ÉÎ µ 4 µ¥¡´œ›»r ŗo„n
AVROS2037 (270 „·Ã¨„¦´¤/Ŧn/že) RRIT402 (230 „·Ã¨„¦´¤/Ŧn/že) BPM3 (200 „·Ã¨„¦´ ¤/Ŧn/že) ¨³KRS138 (200
„·Ã¨„¦´¤/Ŧn/že)×¥ªµŠªµŠÂŸœ„µ¦š—¨°ŠÂ»n ¤˜¨°— (completely randomized design, CRD) ¤¸ 8 š¦¸˜Á¤œ˜rÇ ¨³ 10
ŽÊε Á¤ºÉ°„¨oµ¥µŠ¡µ¦µ¤¸°µ¥» 18 Á—º°œ ‹¹ŠÁ„ȝœÊε¥µŠÅžÁ˜¦¸¥¤ total RNA ¨³ª·Á‡¦µ³®r„µ¦Â—Š°°„ °Š¥¸œ—oª¥Áš‡œ·‡ RT-
PCR ˜´ª°¥nµŠ¨³ 3 ŽÊε
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šÎ µ°·Á¨‡Ãš¦Ã¢¦¸ Ž· RT-PCR product °Š˜´ ª°¥n µŠ˜nµŠÇ ĜÁ‹¨°³„µÃ¦‡ªµ¤Á o¤ o œ 2% ¥o°¤Á‹¨—oª¥
ethidium bromide ™nµ¥¦¼ žÁ‹¨Â¨³ª´—‡nµ intensity °ŠÂ™ DNA ‡n µ„µ¦Â—Š°°„ °Š¥¸œ ‡Îµœª–‹µ„‡nµ°´˜¦µn ªœ
¦³®ªnµŠ intensity °ŠÂ™ DNA ¥¸œÁžjµ®¤µ¥Â¨³ intensity °ŠÂ™ DNA ¥¸œ 18S rRNA (¥¸ œ‡ª‡»¤)
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ª· Á‡¦µ³®ro°¤¼ ¨„µ¦Â—Š°°„ °Š¥¸œÃ—¥„µ¦š—°‡ªµ¤Âž¦ž¦ªœ (ANOVA) ¨³Áž¦¸¥Áš¸¥‡ªµ¤
˜„˜nµŠ‡nµÁŒ¨¸É¥ °Šš¦¸ ˜Á¤œ˜r×¥ª·›¸ Duncan’s Multiple Range Test (DMRT)