เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 307

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥ ¤®µª·š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
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‹Î µÂœ„„µ¦Â—Š°°„ °Š¥¸œÄœœÊε¥µŠ„¨»n¤µ¥¡´ œ›»rÄ®oŸ¨Ÿ¨· ˜¼ŠÂ¨³„¨»n¤Ä®oŸ¨Ÿ¨· ˜˜Éε—oª¥Áš‡œ· ‡ cDNA-
AFLP ץčoÁ°œÅŽ¤r ˜´—‹ÎµÁ¡µ³ EcoRI/PstI ¨³ MseI/Apo I —oª¥Å¡¦Á¤°¦r‡´ —Á¨º°„‹Îµœªœ 40 ‡¼n¨³ 20 ‡¼n ˜µ¤¨Îµ—´
(£µ¡š¸É 1) ŗo™ °Š TDFs š¸É¤¸‡ªµ¤Á o¤¤µ„Äœ„¨»n¤µ¥¡´œ›»rÄ®o Ÿ¨Ÿ¨·˜¼Š‹Îµœªœ 20 TDFs µ¤µ¦™ re-amplified ¨³
ª·Á‡¦µ³®r®µ¨Îµ—´ ÁÅ—o‹Îµœªœ 11 TDFs Á¤ºÉ°Áž¦¸¥Áš¸¥„´ ¨Îµ—´ÁÄœ“µœ o°¤¼¨ (GenBank) ×¥¥°¤¦´ homology
°Š¨Îµ—´ÁÁ¤ºÉ° E value ˜É優n µ 10-3 ¡ªnµ¨Î µ—´Á °Š¥¸œ‹Îµœªœ 5 TDFs homology „´ ¨Îµ—´Á °Š¥¸œÄœ
“µœ o°¤¼ ¨Å—o„n TDFLH73 TDFLH89 TDFLH193 TDFLH363 TDFLH23 nªœ TDFs °ºÉ œÇ no homology (˜µ¦µŠš¸É 1)
—Šªnµ TDFs š¸É no homology °µ‹‹³Áž}œ¥¸œš¸É˜„˜nµŠÅž‹µ„¥¸ œ °Š·ÉŠ¤¸¸ª·˜°ºÉœÇ š¸É¤¸°¥¼nĜ“µœ o°¤¼ ¨ ®¦º°¨Îµ—´Áš¸É
ŗo°µ‹‹³Áž} œnªœš¸ÉŤnž¦¦®´ (non-coding region) Ž¹É ŠÄœ·ÉŠ¤¸¸ª·˜˜nµŠ„´ œ¤´„‹³¤¸¨Î µ—´ÁÄœ¦·Áª–œ¸Ê‡n °œ oµŠ
˜„˜nµŠ„´œÂ¤oªnµ‹³Áž}œ¥¸œÁ—¸ ¥ª„´œ ˜n°¥nµŠÅ¦„Șµ¤¥¸œ °Š TDFs š¸É‡´ —Á¨º°„Å—oš´Ê Š®¤—œnµ‹³¤¸šµšÎµ‡´ ˜n°Ÿ¨Ÿ¨· ˜
œÊÎ µ¥µŠ °Š¥µŠ¡µ¦µ
£µ¡š¸É 1 ˜´ª°¥nµŠ gene expression pattern ĜœÊε¥µŠ °Š¥µŠ¡µ¦µ„¨»n¤µ¥¡´œ›»rÄ®oŸ¨Ÿ¨·˜¼Š (RRIM600 = 3 ¨³
RRIT251 = 4) ¨³„¨»n¤µ¥¡´œ›»rÄ®oŸ¨Ÿ¨·˜˜Éε (BPM3 =1 ¨³ KRS138 = 2) ‹µ„„µ¦šÎ µ cDNA AFLP ¨³˜´ª°¥nµŠÂ™
TDFs š¸É¤¸‡ªµ¤Á o¤¤µ„Äœ„¨»n¤¥µŠ¡µ¦µµ¥¡´ œ›»rÄ®o Ÿ¨Ÿ¨·˜œÊε¥µŠ¼Š («¦¸Ê)
1 2 3 4 1 2 3 4 1 2 3 4
E+AGA/P+GGG E+ACG/P-+GTC E+ACC+/P+GTC