Proceeding2562

1558 การประชุมวิชาการระดับชาติมหาวิทยาลัยทักษิณ ครั้งที่ 29 ประจ�ำปี 2562 วิจัยและนวัตกรรมเพื่อการพัฒนาที่ยั่งยืน Detection of Abnormal hemoglobin; Hemoglobin Hope and Hemoglobin Tak by Polymerase chain reaction combined with high resolution melting curve analysis (PCR-HRM) Panchanok Kaewchoat 1 , Pichaya Chanpool 2 , Sitthichai Panyasai 3 , Patcharawadee Prayalaw 4 and Thanet Prajantasen 4* Abstract Introduction : Abnormal Hemoglobins (Hb) are structural abnormality of hemoglobin. The common abnormal Hemoglobins in Thailand when its excluded Hb E and Hb Constant Spring are Hb Hope and Hb Tak. Although, these abnormal Hb cannot lead to severe thalassemia symptoms, Hb analysis of Hb Hope and Hb Tak are complicated interpretation and lead to misdiagnosis from co-migration of Hb Hope and Hb Tak with others Hb. Objective : To develop the Polymerase chain reaction combined with high resolution melting curve analysis (PCR-HRM) for Hb Hope and Hb Tak detection. Methods : Known DNA samples were used for optimization using PCR-HRM. Further analysis of percentage of concordance was done by 97 blind samples for comparison between the developed PCR-HRM assay and allele specific PCR. Results : Our optimization, HRM profile showed specific pattern of melting curve. Further the comparison between PCR-HRM assay and allele specific PCR was done by 97 samples with 100 % accordance. Conclusion : Therefore, our developed HRM should be useful and applied for Hb Hope and Hb Tak detection in thalassemia laboratory. Keyword : Hb Hope, Hb Tak, High resolution melting curve (HRM) analysis, Abnormal Hemoglobin