เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 793

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´ „¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
5
3. „µ¦š—°Ÿ¨ °Š°»–®£¼¤·š¸É¤¸˜n°§š›·Í°Šµ¦¨³¨µ¥ŸŠ„´—
Á˜·¤°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Á®¨ª MHB ¨ŠÄœ 96-wells „oœÂœ (flat bottom microtitration plate) Ĝ®¨»¤š¸É
2-13 ž¦· ¤µ˜¦®¨»¤¨³ 100 ŤÇ¦¨· ˜¦ ‹µ„œ´ÊœÂ¥„šÎ µÁ‹º°‹µŠÂ°ŠÁšnµ °Šµ¦¨³¨µ¥ŸŠ„´—š»„ž¦³Á£š
(µ¦¨³¨µ¥¨µ¥ŸŠ„´— 35% Á°šµœ°¨ µ¦¨³¨µ¥ŸŠ„´—œÊε µ¦¨³¨µ¥ŸŠ„´—Á‡¸É¥ªœÊε) ¨³µ¦¨³¨µ¥š» „
ž¦³Á£šš¸ÉŸnµœž{‹‹´¥š»„ž{‹‹´¥ (°»–®£¼¤· pH) ×¥„µ¦Á˜·¤µ¦¨³¨µ¥ŸŠ„´—¨ŠÅžÄœ®¨» ¤š¸É 1 ¨³ 2 ž¦· ¤µ˜¦®¨»¤¨³
100 ŤÇ¦¨·˜¦ Ÿ¤µ¦Äœ®¨»¤š¸É 2 Ä®o Á oµ„´œ°¥nµŠ—¸ ¨oª—¼—nªœŸ¤ °Š®¨»¤š¸É 2 ž¦·¤µ˜¦ 100 ŤÇ¦¨·˜¦ Än
Ĝ®¨»¤š¸É 3 Ÿ¤Ä®oÁ oµ„´œ°¥nµŠ—¸ ¨oª—¼—nªœŸ¤ÄnĜ®¨»¤š¸É 4 ž¦· ¤µ˜¦ 100 ŤÇ¦¨·˜¦ ¨o ªÁ‹º°‹µŠ˜n°ÅžÃ—¥
šÎ µŽÊε„´œ‹œ‡¦š´ÊŠ 10 ®¨»¤ —¼—nªœŸ¤Äœ®¨»¤š¸É 10 š·ÊŠÅž 100 ŤÇ¦¨·˜¦ ‹µ„œ´ÊœÁ˜· ¤µ¦Â ªœ¨°¥ÁºÊ°
S. aureus , E. coli, B. subtilis ®¦º ° C. albicans ¨ŠÄœš»„®¨»¤ ž¦·¤µ˜¦®¨» ¤¨³ 100 ŤÇ¦¨·˜¦ Ÿ¤Ä®oÁ oµ„´œ
n ¤Á¨¸Ê¥ŠÁºÊ°š¸É°»–®£¼¤· 37 °Š«µÁŽ¨ÁŽ¸¥ Áž}œÁª¨µ 24 ´ÉªÃ¤Š °nµœ‡nµ MIC ×¥´ŠÁ„˜‡ªµ¤ »nœ Áž¦¸¥Áš¸¥‡nµš¸É ŗo
„´‡nµš¸Éŗo‹µ„„µ¦š—°—o ª¥¥µ˜oµœ‹»¨¸¡ (Amphotericin B; Kanamycin; Gentamicin : Fluka)
®¨»¤š¸É 1 Áž}œ negative control ž¦³„°—oª¥µ¦„´ —¤»œÅ¡¦Â˜n ¨³œ·—„´ ÁºÊ°—´œ¸(Ťn¤¸°µ®µ¦Á¨¸Ê¥ŠÁºÊ °)
®¨»¤š¸É 11 Áž}œ positive control ž¦³„°—o ª¥°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Á®¨ª„´ ÁºÊ°—´œ¸ (Ťn¤¸ µ¦„´—¤»œÅ¡¦) ®¨»¤š¸É 12
Áž}œ negative control ž¦³„°—oª¥ 100% DMSO „´ÁºÊ°—´ œ¸ (—¼‡ªµ¤Áž}œ¡·¬ °Š DMSO) ®¨»¤š¸É 13 Áž} œ
negative control ž¦³„°—oª¥ œÊÎ µ„¨´Éœž¦µ«‹µ„ÁºÊ°„´ÁºÊ °—´œ¸ (—¼‡ªµ¤Áž}œ¡·¬ °ŠœÊε)
°nµœ‡nµ‡ªµ¤Á o¤ oœ˜Éε» — °Šµ¦„´—¤»œÅ¡¦š¸ÉšÎµ¨µ¥‹»¨·œš¦¸¥r (MBC/MFC) ×¥—¼—µ¦Ÿ¤‹µ„®¨»¤
MIC ¨³™´— ¹Êœ¤µ°¸„ 3 ®¨»¤ ž¦· ¤µ˜¦®¨»¤¨³ 100 ŤÇ¦¨·˜¦ ¤µ„¦³‹µ¥¨Šœ¡ºÊœŸ·ª°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Â Ȋ NA
宦´ ÁºÊ°Â‡š¸Á¦¸¥ S. aureus, E. coli ¨³ B. subtilis ¨³°µ®µ¦Á¨¸Ê¥ŠÁºÊ °Â Ȋ SDA 宦´ ÁºÊ°¦µ C. albicans
Á¡µ³Á¨¸Ê¥ŠÁºÊ°Åªoš¸É °»–®£¼¤· 37 °Š«µÁŽ¨ÁŽ¸¥Áž}œÁª¨µ24 ´ÉªÃ¤Š °nµœ‡nµ MBC/MFC š¸É‡ªµ¤Á o¤ oœ˜Éε»—š¸É¤¸ÁºÊ°
Á‹¦·œo°¥„ªnµ 5 Ç蜸 œ‹µœÁ¡µ³ÁºÊ° (°£·¦µ¤ ¨³ ¤–”¨, 2545)
Ÿ¨„µ¦ª·‹´¥Â¨³°£·ž¦µ¥Ÿ¨
Ÿ¨„µ¦«¹„¬µ§š›·Í˜oµœÁºÊ°—´œ¸°Šµ¦„´—¤»œÅ¡¦
1. ×¥ª·›¸ agar–well diffusion
‹µ„„µ¦š—°§š›·Í°Šµ¦„´—‹µ„·Êœnªœ¤» œÅ¡¦Á‡¸É¥ª‹Îµœªœ 5 œ· — ŗo„n Á®Šº°„ž¨µ®¤°
³Á°¤-Áš« „¦³šº° ¢jµš³¨µ¥Ã‹¦ ¨³´ª„ Ĝ„µ¦˜o µœÁºÊ°—´ œ¸‹Îµœªœ 4 œ·— ŗo „n S. aureus, E. coli,
B. subtilis ¨³ C. albicans —oª¥ª·›¸ agar-well diffusion ¡ªnµµ¦„´—‹µ„·Êœnªœ³Á°¤Áš«Á‡¸É¥ª¤¸§š›·Í˜oµœ
S. aureus ¨³ B. subtilis ץĮo œµ—ªŠÄ °Š„µ¦¥´¥´Ê Š (inhibition zone) Ášnµ„´œ‡º° 19 ¤·¨¨·Á¤˜¦ (¤¤.) ¨³µ¦
„´—·Ê œnªœÁ®Šº°„ž¨µ®¤°Á‡¸É ¥ª¤¸§š›·Í˜oµœÁŒ¡µ³ S. aureus ץĮoœµ— °Š inhibition zone Ášnµ„´ 11 ¤¤.
(˜µ¦µŠš¸É 1) ¨³Á¤ºÉ °œÎµŸŠ¤»œÅ¡¦š´Ê Š 5 œ·—¤µÁ‡¸É¥ª—oª¥œÊε ¡ªnµµ¦„´ —ŸŠÁ®Šº°„ž¨µ®¤°Á‡¸É¥ª¥´Š‡Š¤¸§š›·Í
˜oµœ S. aureus ŗoץĮoœµ— °Š inhibition zone Ášnµ„´ 15 ¤¤. ¨³µ¦„´—ŸŠ³Á°¤Áš«Á‡¸É ¥ª¤¸§š›·Í˜oµœ
S. aureus ¨³ B. subtilis ץĮoœµ— °Š inhibition zone Ášnµ„´ 16 ¨³ 12 ¤¤. ˜µ¤¨Îµ—´ (˜µ¦µŠš¸É 1)