full2010.pdf - page 117

79
O10
„µ¦‡´—Á¨º°„¥¸œš¸É¤¸„µ¦Â—Š°°„˜„˜nµŠ„´ œÄœÁž¨º°„°n°œ¥µŠ¡µ¦µš¸É¤¸°µ„µ¦Áž¨º°„®oŠÂ¨³ž„˜·
×¥Áš‡œ· ‡ cDNA-AFLP
Selection of Differentially Expressed Genes in Soft Bark of Tapping Panel Dryness
and Normal Para Rubber Tree using cDNA-AFLP Technique
£·¦¤¥rµ —ŠªnµŠ1, ‹µ¦»ª´˜¦ ‹´œš¦rž¦³—·¬“r2¨³Áž¨ºÊ°Š »ª¦¦–¤–¸2*
Phiromya Daengsawang1 Charuvat Chantrapradist2, Pluang Suwanmanee 2*
š‡´—¥n°
‹µ„„µ¦ª·Á‡¦µ³®r„µ¦Â—Š°°„ °Š¥¸œÄœÁž¨º°„°n °œ¥µŠ¡µ¦µš¸É¤¸°µ„µ¦Áž¨º°„®oŠ(Tapping panel dryness
syndrome; TPD) Áž¦¸¥Áš¸ ¥„´˜oœž„˜·Ã—¥ÄoÁš‡œ·‡ cDNA-AFLP ¡ transcription derived fragments (TDFs) š¸É ¤¸
‡ªµ¤Á o¤¤µ„Äœ„¨»n¤°µ„µ¦Áž¨º°„®oŠ‹Îµœªœ 20 TDFs ¨³„¨»n¤ž„˜· ‹Îµœªœ 7 TDFs µ¤µ¦™Ã‡¨œÂ¨³ª·Á‡¦µ³®r ¨Îµ—´ 
ÁÅ—o 19 TDFs Á¤ºÉ°Áž¦¸¥Áš¸¥¨Îµ—´Á„´¥¸œÄœ“µœ o °¤¼¨¡ªn µ¤¸ 9 TDFs ¤¸¨Îµ—´Á homology „´¥¸ œÄœ
“µœ o°¤¼¨ GenBank Á¤ºÉ°˜¦ª‹°¦¼žÂ„µ¦Â—Š°°„ °Š¥¸œ—oª¥Áš‡œ·‡ RT-PCR ¡ªnµ´¤¡´œ›r„´  cDNA-AFLP
‡ÎµÎµ‡´ : „µ¦Â—Š°°„ °Š¥¸œ, ¥µŠ¡µ¦µ, „µ¦Á„·—°µ„µ¦Áž¨º°„®oŠ °Š¥µŠ¡µ¦µ
Abstract
Complementary DNA-AFLP (cDNA-AFLP) was used to identify different levels of transcripts from soft
bark of tapping panel dryness (TPD) and healthy rubber trees. Twenty-seven differentially expressed transcript-
derived fragments (TDFs) were obtained. Twenty of these TDFs were high intensity in TPD rubber tree and seven in
healthy ones. When the differentially expressed TDFs were cloned and sequenced, nineteen TDFs produced readable
sequences. Only nine TDFs revealed homology with known genes while another showed no significant homology to
any sequences in GenBank. The relationship between cDNA-AFLP expression pattern and RT-PCR were confirmed.
Keywords: Gene expression, Hevea brasiliensis , Tapping panel dryness (TPD)
Ɯ´ „«¹„¬µž¦·µÃš µ µ¸ªª· š¥µ £µ‡ª·µ¸ªª·š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´ ¥š´„¬·– Š ¨µ 90000
Master’s Degree Student. Department of Biology, Faculty of science, Thaksin University, Songkhla, 90000.
2°µ‹µ¦¥r£µ‡ª·µ¸ªª·š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´ ¥š´„¬·– Š ¨µ 90000
Dr., Department of Biology, Faculty of science, Thaksin University, Songkhla, 90000.
*corresponding e-mail:suwanmaneep@yahoo.com