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„µ¦Â—Š°°„ °Š TDFREP241 š¸É homology „´¥¸ œ ankyrin-repeat domain zinc finger protein
(KR1) Ĝ¥µŠ¡µ¦µ (Hevea brasiliensis (Willd. ex A.Juss.) Muell. Arg.)
The Expression of TDFREP241 Homologue of Ankyrin-Repeat Domain Zinc Finger Protein
(KR1) Gene from Hevea brasiliensis (Willd. ex A.Juss.) Muell. Arg.
°µ—¸®¨³ Á‹³Á¤µ³1, °¦»–¦´«¤·Í ª–·µœœšr2 ¨³Áž¨ºÊ°Š »ª¦¦–¤–¸2*
Adilah Jehmoh1, Arunrut Vanichanon2 and Pluang Suwanmanee2*
š‡´—¥n °
TDFREP241 Áž}œ transcription derived fragment (TDF) š¸É¤¸„µ¦Â—Š°°„¤µ„Äœ¥µŠ¡µ¦µµ¥¡´ œ›»rÄ®o
Ÿ¨Ÿ¨·˜œÊε¥µŠ¼Š ¤¸¨Îµ—´Á homology „´¥¸œ ankyrin-repeat domain zinc finger protein (KR1 ) Šµœª·‹´¥œ¸Ê˜o°Š„µ¦
ª·Á‡¦µ³®r„µ¦Â—Š°°„ °Š TDFREP241 Ĝ¥µŠ¡µ¦µ ×¥Áš‡œ·‡ semi-quantitative RT-PCR Ÿ¨„µ¦«¹„¬µ¡ªn µ
ÁœºÊ°Á¥ºÉ°Ä „oµœÄ ¨Îµ˜oœÂ¨³¦µ„ °Š„¨oµ¥µŠ¡µ¦µ¤¸„µ¦Â—Š°°„ °Š TDFREP241 ˜„˜n µŠ„´œ°¥nµŠ¤¸œ´ ¥Îµ‡´
šµŠ™·˜·š¸É¦³—´ 0.05 宦´˜oœ„¨oµ¥µŠ¡µ¦µš¸Éŗo ¦´ÃŽÁ—¸¥¤‡¨°Å¦—r‡ªµ¤Á o¤ oœ 0.5 䨵¦r ¤¸„µ¦Â—Š°°„
°Š¥¸ œÁ¡·É¤ ¹Êœž¦³¤µ– 3 Ášnµš¸ÉÁª¨µ 24 ´É ªÃ¤Š nªœ˜oœ„¨oµ¥µŠ¡µ¦µš¸Éŗo ¦´Ã¡¨¸Á°›¸ ¨¸œÅ„¨‡°¨ ¤¸„µ¦Â—Š°°„
°Š¥¸ œÁ¡·É¤ ¹Êœž¦³¤µ– 2 Ášnµ ¥µŠ¡µ¦µ˜·—˜µ°µ¥»ž¦³¤µ– 24 Á—º°œ š¸É„¦¸ —™¸É („¦¸— 1 ‡¦´ÊŠ˜n°ª´ œ ) ¤¸ „µ¦Â—Š°°„
°Š¥¸ œ¤µ„„ªnµ¥µŠ¡µ¦µš¸É „¦¸—ž„˜· („¦¸—1 ‡¦´ÊŠ˜n° 3 ª´œ) °¥nµŠ¤¸ œ´¥Îµ‡´šµŠ™·˜·š¸É ¦³—´ 0.05 œ°„‹µ„œ¸Ê¥´Š¡ªnµ
TDFREP241 ¤¸ „µ¦Â—Š°°„ÄœœÊε¥µŠ °Š¥µŠ¡µ¦µ˜·—˜µ °µ¥» 18 Á—º °œ µ¥¡´œ›»rÄ®oŸ¨Ÿ¨·˜œÊε¥µŠ¼Š¤µ„„ªnµµ¥
¡´ œ›»rÄ®oŸ¨Ÿ¨· ˜œÊε¥µŠ˜ÉÎ µ°¥nµŠ¤¸œ´ ¥Îµ‡´šµŠ™· ˜·š¸É¦³—´ 0.05 —Šªnµ„µ¦Â—Š°°„ °Š TDFREP241 œnµ‹³¤¸
šµšÎ µ‡´Äœ„µ¦˜°œ°Š˜n° biotic stress ¨³ abiotic stress °Š¥µŠ¡µ¦µ
‡ÎµÎµ‡´ : Ankyrin-repeat domain zinc finger protein (KR1) ¥µŠ¡µ¦µ „µ¦Â—Š°°„ °Š¥¸œ
Abstract
TDFREP241 is an up-regulated expression transcription derived fragment (TDF) in high yielding Hevea
clone. The sequence of TDFREP241 is high homology to ankyrin-repeat domain zinc finger protein (KR1) gene.
The aim of this research is to study the expression pattern of TDFREP241 in Hevea brasiliensis by semi-
quantitative RT-PCR technique. The expression of TDFREP241 in various tissues of Hevea seedlings was
significant difference.
1œ· ·˜ž¦·µÃš µ µª·µ¸ ªª·š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´¥š´„¬·– Š ¨µ 90000
Master’s Degree Student. Department of Biology, Faculty of Science, Thaksin University, Songkhla, 90000.
2°µ‹µ¦¥r µ µª·µ¸ªª· š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª· š¥µ¨´¥š´„¬·– Š ¨µ 90000
Dr., Department of Biology, Faculty of Science, Thaksin University, Songkhla, 90000.
*corresponding e-mail:suwanmaneep@yahoo.com