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˜ÎµÂ®œn Š °Š polyadenylation signal (AAUAAA) °¥¼n¦·Áª–¨Î µ—´Áš¸É 476-481 ¨³¤¸ ᨸ°³—¸œ¸ œ (Poly A)
‹Îµœªœ 24 Á —ŠªnµÁž}œ¨Îµ—´Á °Š¥¸œš¸É ¤¼ ¦–r (complete sequence) (£µ¡š¸É 1) ¨Îµ—´Á °Š¥¸œš¸ÉǨœ
—oª¥Áš‡œ·‡ RACE °µ‹¤¸ ‡ªµ¤Ÿ·—¡¨µ— ‹¹ Š˜¦ª‹°°¸„‡¦´ÊŠ®œ¹ÉŠÃ—¥Äo¨Îµ—´Áš¸Éŗo‹µ„ 5c-RACE ¨³ 3c-RACE
Áž}œÂÄœ„µ¦Á¡·É¤ž¦·¤µ–¥¸ œÄ®o‡¦°‡¨»¤¦·Áª– open reading flame šÎµ„µ¦Ã‡¨œÂ¨³ª·Á‡¦µ³®r¨Îµ—´Á ¡ªnµ
¤¸¨Îµ—´Á˜¦Š„´š¸ÉǨœÅªo „n°œÂ¨oªš´ÊŠ®¤— Þ¦˜¸œ °Š HbCCH ¤¸œÊÎ µ®œ´„äÁ¨„»¨Ášnµ„´ 9.2 kDa Ą¨oÁ‡¸¥Š„´
Þ¦˜¸œ PoCCH °Š poplar (8.9 kDa) ¨³ LeCCH °Š¤³Á º°Áš« (8.5 kDa) –³œ¸Êŗo nŠ o°¤¼¨¨Îµ—´Á °Š¥¸œ
HbCCH Ş¥´ Š GenBank ¨³Å—o¦´„µ¦˜°¦´Ä®o ¤¸„µ¦Á„ȝ o°¤¼¨ÅªoĜ“µœ o°¤¼¨ °Š GenBank Á¦¸¥¦o °¥Â¨oª
Accession Number °Šœ·ª‡¨¸Ã°Åš—r¨³„¦—°³¤·ÃœÅ—o„n GU550955 ¨³ ADD69807 ˜µ¤¨Îµ—´
£µ¡š¸É 1 ¨Îµ—´ÁÂ¨³„¦—°³¤·Ãœ °Š¥¸ œ HbCCH ‹Îµœªœ 567 ‡¼n Á ž¦³„°—oª¥ 5c-noncoding region
coding region ¨³ 3c -noncoding region
Áž¦¸¥Áš¸¥¨Îµ—´„¦—°³¤· Ü °ŠÃž¦˜¸œ HbCCH „´ CCH °Š¡ºÂ¨³·ÉŠ¤¸¸ª·˜°ºÉœÇ
Á¤ºÉ°Áž¦¸¥Áš¸¥¨Îµ—´„¦—°³¤·Ãœ °ŠÃž¦˜¸œ HbCCH „´Ãž¦˜¸œ CCH °Š¡ºÂ¨³·ÉŠ¤¸¸ª·˜°ºÉœÇ ץčo
ަ„¦¤ BioEdit version 7.0.9 (Hall, 1999) ¡ªnµ¨Îµ—´„¦—°³¤·Ãœ °Š HbCCH ¤¸ homology „´ Ãž¦˜¸œ CCH
°Š Populus , Jatropha, Ricinus, Citrus, Solanum, Lycopersicon, Zeamays, Plantago, Arabidopsis, Glycine, Homo
¨³ Saccharomyces Ášnµ„´ 93%, 88%, 87%, 78%, 77%, 77%, 74%, 63%, 53%, 53%, 32% ¨³ 26% ˜µ¤¨Îµ—´
¡ªnµž¦³„°—o ª¥˜ÎµÂ®œn ŠÎµ‡´š¸ÉÁ„¸É¥ª„´„µ¦ œnŠ›µ˜» š°ŠÂ—Š 2 ¦·Áª–—o ª¥„´œ ‡º° ¦·Áª– N – terminal Áž}œ
˜ÎµÂ®œnŠ š¸É ‹´„´›µ˜»š°ŠÂ—Š (metal binding domain; MXCXXC) (Lin and Culotta 1995; Pufahl et al.,1997; Mira
et al ., 2001b) ¨³¦·Áª– C-terminal Áž}œ˜ÎµÂ®œn Š lysine-rich Ž¹ÉŠš´ÊŠ°Š¦·Áª–—´Š„¨nµª¨Îµ—´„¦—°³¤·Ãœ °Š
HbCCH ‹³Á®¤º°œ„´Ãž¦˜¸œ CCH °Š·ÉŠ¤¸¸ª·˜°ºÉœÇ (£µ¡š¸É 2) —Šªnµ¥¸œš¸ÉǨœÅ—oĜ‡¦´ÊŠœ¸ÊÁž}œ¥¸œ °ŠÃž¦˜¸œ
CCH ˜n °¥nµŠÅ¦„Șµ¤„¦—°³¤·Ãœ¦· Áª–—oµœ C-terminal °Š HbCCH ‹³¤¸‹Îµœªœœo°¥„ªnµ Arabidopsis, Glycine
¨³ Plantago ‹Îµœªœ 35, 44 ¨³ 23 „¦—°³¤·Ãœ ˜µ¤¨Îµ—´ ˜n¤¸‹ÎµœªœÄ„¨o Á‡¸¥Š„´¡º°ºÉ œÇ ¦ª¤š´ÊŠ Homo ¨³
Saccharomyces