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«¹„¬µ°»–®£¼¤·š¸É Á®¤µ³¤Äœ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽ œ°µ®µ¦Â Ȋ DSM
Á¨¸Ê¥ŠÁºÊ ° B. subtilis ORB7106 Ĝ°µ®µ¦Á®¨ª Difco sporulation medium (DSM) Ä®oÁ‹¦·°¥¼nĜnªŠž¨µ¥
Exponential phase ®¦º° nªŠ˜oœ Stationary phase œÎµÁºÊ°Â‡š¸ Á¦¸¥š¸Éŗo ¤µÁ¡µ³Á¨¸Ê¥Š—oª¥ª·›¸ Spot inoculation œ
°µ®µ¦Â Ȋ DSM š¸ÉŸ¤¸¥o°¤Á°ÃŽÂ˜n¨³œ·—Å—o „n Azobenzene, Methyl red, Orange G ¨³ Congo red š¸É‡ªµ¤
Á o¤ oœÁ¦·É¤˜oœ 10, 50, 100 ¨³ 200 ¤·¨¨·„¦´ ¤˜n°¨·˜¦ š¸É¡¸ Á° 7 ‹µ„œ´ÊœœÎµ¤µn¤š¸É°»–®£¼¤·˜nµŠÇ ˜´Ê ŠÂ˜n 25, 30, 37, 40
¨³ 45 °Š«µÁŽ¨ÁŽ¸ ¥ Áž}œÁª¨µ 48 ´ÉªÃ¤Š ´ ŠÁ„˜¦·Áª–Ħ°Ã‡Ã¨œ¸œŸ· ª®œoµ°µ®µ¦
«¹„¬µ¡¸ Á°š¸ÉÁ®¤µ³¤Äœ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽœ°µ®µ¦Â Ȋ DSM
Á¨¸Ê¥ŠÁºÊ ° B. subtilis ORB7106 Ĝ°µ®µ¦Á®¨ª DSM Ä®oÁ‹¦·°¥¼nĜnªŠž¨µ¥ Exponential phase ®¦º° nªŠ
˜oœ Stationary phase œÎµÁºÊ°Â‡š¸Á¦¸¥š¸É ŗo¤µÁ¡µ³Á¨¸Ê¥Š—oª¥ª·›¸ Spot inoculation œ°µ®µ¦Â Ȋ DSM š¸ÉŸ¤¸¥o°¤
Á°ÃŽÂ˜n¨³œ·—Å—o„n Azobenzene, Methyl red, Orange G ¨³ Congo red š¸É‡ªµ¤Á o ¤ oœÁ¦·É¤˜oœ 10, 50, 100 ¨³
200 ¤·¨¨·„¦´ ¤˜n°¨·˜¦ š¸É¡¸ Á° 5, 7 ¨³ 9 ×¥ž¦´¡¸Á°—o ª¥ Acetate ¨³ Phosphate buffer ‹µ„œ´ÊœœÎµ¤µn¤š¸É
°»–®£¼¤· 37 °Š«µÁŽ¨ÁŽ¸¥ Áž}œÁª¨µ 48 ´ÉªÃ¤Š ´ ŠÁ„˜¦·Áª–Ħ°Ã‡Ã¨œ¸œŸ·ª®œoµ°µ®µ¦
„µ¦š—°‡ªµ¤Á o ¤ oœÁ¦·É¤˜oœÄœ„µ¦¥n °¥¨µ¥¸¥o °¤Á°ÃŽÄœ°µ®µ¦Á®¨ª DSM
Á¨¸Ê¥ŠÁºÊ ° B. subtilis ORB7106 ¨³ B. subtilis JH642 š¸É¤¸ž¦·¤µ–ÁŽ¨¨r ÁºÊ°Á¦·É¤˜oœÁšnµ„´ 107 CFU/ml Ĝ
°µ®µ¦Á®¨ª DSM ž¦·¤µ˜¦ 100 ¤·¨¨·¨·˜¦ š¸ÉŸ¤¸¥o°¤Á°ÃŽÂ˜n¨³œ·—Å—o„n Azobenzene, Methyl red, Orange G
¨³ Congo red š¸É ‡ªµ¤Á o¤ oœÁ¦·É¤˜oœ 10, 50, 100 ¨³ 200 ¤·¨¨· „¦´¤˜n°¨·˜¦ š¸É¡¸ Á° 7 ¨³°»–®£¼¤· 37 °Š«µ
ÁŽ¨ÁŽ¸¥ Ĝ£µª³‡Šš¸É Á„ȝ˜´ª°¥n µŠš»„ 4 ´É ªÃ¤Š Áž}œÁª¨µ 48 ´ÉªÃ¤Š œÎµ˜´ª°¥nµŠš¸Éŗo ޞ{~œÁ®ª¸É ¥Šš¸É‡ªµ¤Á¦Èª 5,000
¦°˜n °œµš¸ Áž}œÁª¨µ 5 œµš¸ Á¡ºÉ°˜„˜³„°œÁŽ¨¨r ‹µ„œ´ÊœœÎµµ¦¨³¨µ¥š¸Éŗoު· Á‡¦µ³®r‡nµ„µ¦—¼—„¨ºœÂŠ ץčo
‡ªµ¤¥µª‡¨ºÉ œ °Š¸¥o°¤Á°ÃŽÂ˜n¨³œ·— š¸É 380, 430, 480 ¨³ 490 œµÃœÁ¤˜¦˜µ¤¨Îµ—´ (Sugiura et al., 2006) Á¡ºÉ°
ª´—ž¦³·š›·£µ¡Äœ„µ¦¥n°¥¨µ¥
ª·›¸ „µ¦ª´—ž¦³·š›·£µ¡Äœ„µ¦¥n°¥¨µ¥ (Wang et al., 2008)
ž¦³·š›·£µ¡„µ¦¥n°¥¨µ¥ (%) =
Á¤ºÉ° A i ‡º° ‡nµ„µ¦—¼—„¨ºœÂŠ„n°œÁ„·—„µ¦¥n°¥¨µ¥
At ‡º° ‡nµ„µ¦—¼—„¨ºœÂŠ®¨´ŠÁ„·—„µ¦¥n°¥¨µ¥ –. Áª¨µ˜nµŠÇ
«¹„¬µ°»–®£¼¤·š¸É Á®¤µ³¤Äœ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽ Ĝ°µ®µ¦Á®¨ª DSM
Á¨¸Ê¥ŠÁºÊ ° B. subtilis ORB7106 ¨³ B. subtilis JH642 š¸É¤¸ž¦·¤µ–ÁŽ¨¨r ÁºÊ°Á¦·É¤˜oœÁšnµ„´ 107 CFU/ml Ĝ
°µ®µ¦Á®¨ª DSM ž¦·¤µ˜¦ 100 ¤·¨¨·¨·˜¦ š¸ÉŸ¤¸¥o°¤Á°ÃŽÂ˜n¨³œ·—Å—o„n Azobenzene, Methyl red, Orange G
¨³ Congo red š¸É‡ªµ¤Á o ¤ oœÁ¦·É¤˜oœ 10, 50, 100 ¨³ 200 ¤·¨¨·„¦´¤˜n°¨·˜¦ š¸É¡¸ Á° 7 ¨³°»–®£¼¤·˜nµŠÇ ˜´ÊŠÂ˜n 25,
37 ¨³ 45 °Š«µÁŽ¨ÁŽ¸ ¥ Ĝ£µª³‡Šš¸É Á„ȝ˜´ª°¥nµŠš»„ 4 ´ÉªÃ¤Š Áž} œÁª¨µ 48 ´ÉªÃ¤Š œÎµ˜´ª°¥n µŠš¸Éŗoޞ{~œÁ®ª¸É¥Š
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