51
potential natural source as anticancer treatment. The potential effects apoptosis in Shiraz red pomace extract
using a MCF-7 cell model was determined on the nuclear morphology change, DNA fragmentation and the
expression of apoptotic proteins, Bcl-2 and p53. After 5 hours of the incubation with pomace extract, the nuclei of
treated cells were fragmented with blebbing and the ladder-like DNA fragments. Pomace extract induced the
expressions of p53 (53 kDa) apoptotic proteins and reduced the expressions of Bcl-2 (26 kDa). The results were
expected that such a multi-marker analysis of apoptosis pathway could be useful for individualization of
therapeutic strategies in the future.
References
Alkhalaf, M. (2007). Resveratrol-induced apoptosis is associated with activation of p53 and inhibition of protein
translation in T47D human breast cancer cells.
Pharmacology.
80, 134-143.
Baumgartel, T., Kluth, H., Epperlein, K. and Rodehutscord, M. (2005). A note on digestibility and energy value
for sheep of different grape pomace.
Food Research International
62, 302-306.
Burns, J., Garddner, P.T., O’Neil, J., Crawford, S., Morecroft, I., McPhail, D.B., Lister, C., Matthews, D.,
MacLean, M.R., Lean, M.E.J., Duthie, G G. and Crozier, A. (2000). Relationship among antioxidant,
vasodilation capacity, and phenolic content of red wines.
Journal of Agricultural Food Chemistry
48,
220-230.
Cantos, E., Espin, J.C. and Tomas-Barberan, F.A. (2002). Varietal differences among the polyphenol profiles of
seven table grape cultivars studies by LC-DAD-MS-MS.
Journal of Agricultural Food Chemistry
50,
5691-5696.
Du, Y. and Lou, H. (2008). Catechin and proanthocyanidin B4 from grape seeds prevent doxorubicin-induced
toxicity in cardiomyocytes.
European Journal of Pharmacology
591, 96-101.
Hansen, M.B., Nielsen, S.E. and Berg, K. (1989). Re-examination and further development of a precise and rapid
dye method for measuring cell growth/cell kill.
Journal of Immunological Methods
119, 203-210.
Hata, K., Hori, K. and Takahashi, S. (2002). Differentiation- and apoptosis-inducing activities by pentacyclic
triterpenes on a mouse melanoma cell line.
Journal of Natural Products
65, 645-648.
Hockenbery, D.M. (1995). bcl-2, a novel regulator of cell death.
BioEssays.
17, 631-638.
Kanner, J., Frankel, E., Granit, R., German, B. and Kinsella, J.E. (1994). Natural antioxidants in grapes and wines.
Ibid.
42, 64-69.
Laemmli, U.K. (1970). Cleavage of structural proteins during assembly of head of bacteriophage-T4.
Nature
227,
680-685.
Makris, D., Boskou, G. and Andrikopoulos, N. (2007). Polyphenolic content and
in vitro
antioxidant
characteristics of wine industry and other agri-food solid waste extracts.
Journal of Food Composition
and Analysis
20, 125-32.
