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„µ¦¥n °¥¨µ¥¸¥o°¤ž¦³Á£šÁ°ÃŽ —oª¥Á°ÃŽ¦¸—´„Áš (AzoR1)
‹µ„‡š¸Á¦¸¥ Bacillus subtilis ORB7106
Decolorization of Azo Dyes by Azoreductase (AzoR1) from Bacillus subtilis ORB7106
»„´¨¥µ ¦· »š›·Í 1* ¤œš·¦µ ¨¸¨µÁ„¦¸ ¥Š«´„—·Í1 ¨³¤¦´„¬r ¡´ œ›rŸ¨ 1
Sukallaya Borisut1* Montira Leelakriangsak1 and Somrak Panphon1
š‡´—¥n °
¸¥o°¤Á°ÃŽÁž} œµ¦ž¦³„°°³Ã¦¤µ˜·„š¸É¤¸®¤¼n -N=N- ĜǦŠ¦o µŠ œ·¥¤Äo „´œ°¥n µŠÂ¡¦n®¨µ¥Äœ
°»˜µ®„¦¦¤˜nµŠÇ Ánœ °»˜µ®„¦¦¤·ÉŠš° °»˜µ®„¦¦¤—oµœ°µ®µ¦ °»˜µ®„¦¦¤·ÉŠ¡· ¤¡r °»˜µ®„¦¦¤„µ¦´„
¨³°» ˜µ®„¦¦¤Á‡¦ºÉ°ŠÎµ°µŠ ‹¹ŠšÎµÄ®oœÊεš·ÊŠ‹µ„°»˜µ®„¦¦¤Á®¨nµœ¸Ê ¤¸ ¸¥o°¤Á°ÃŽ®¨µ¥œ·—žœÁžg°œ°¥¼ n¨³nŠŸ¨
Ä®o Á„· —¤¨¡·¬˜n°·É ŠÂª—¨o °¤ ˜n°¤µ¤¸ „µ¦«¹ „¬µ„µ¦‡ª‡»¤„µ¦Â—Š°°„ °Š¥¸ œ azoR1 Ĝ‡š¸Á¦¸¥ Bacillus
subtilis ¨³¡ªnµ ¥¸ œ azoR1 ´ ŠÁ‡¦µ³®rÁž}œÃž¦˜¸œš¸É¤¸¨Îµ—´„¦—°³¤·Ãœ‡¨oµ¥‡¨¹Š„´ FMN—dependent NADH-
azoreductase Ĝ„µ¦¦o µŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš Á¦¸¥„ªn µ AzoR1 Á°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš Áž}œÁ°œÅŽ¤rš¸É µ¤µ¦™¥n°¥
¨µ¥¸¥o°¤Á°ÃŽÅ—o ×¥„µ¦˜´—¡´ œ›³ °Š®¤¼nÁ°ÃŽ ‹µ„„µ¦ª· Á‡¦µ³®r×¥ª·›¸ ŤÇ¦°µÁ¦¥r ¨³Ãž¦˜· 𤷄 ¡ªn µ
ž¦· ¤µ– °Š¥¸œ azoR1 ¨³Ãž¦˜¸œ AzoR1 ¤¸ ž¦·¤µ–Á¡·É¤ ¹ÊœÄœ B. subtilis µ¥¡´œ›»r ORB7106 ( yodB ¤·ªÁš´ œ) Ĝ
„µ¦«¹ „¬µœ¸Ê ‹¹ŠÁž} œ„µ¦«¹„¬µ‡ªµ¤µ¤µ¦™ °Š B. subtilis ORB7106 Ĝ„µ¦¥n°¥¨µ¥¸ ¥o°¤Á°ÃŽž¦³Á£š
Azobenzene, Methyl red, Orange G ¨³ Congo red š¸É‡ªµ¤Á o ¤ oœ 10, 50, 100 ¨³ 200 ¤·¨¨·„¦´ ¤˜n°¨· ˜¦ ×¥
«¹„¬µ„µ¦¥n°¥¨µ¥¸¥o°¤Äœ£µª³š¸É˜„˜nµŠ„´œ °Š°»–®£¼¤· ˜´ÊŠÂ˜n 25, 30, 37, 40 ¨³ 45 °Š«µÁŽ¨ÁŽ¸¥Â¨³ š¸É
¡¸Á° 5, 7 ¨³ 9 œ°µ®µ¦Â È ŠÂ¨³Äœ°µ®µ¦Á®¨ª ‹µ„„µ¦š—°œ°µ®µ¦Â Ȋ ¡ªnµ B. subtilis ORB7106
µ¤µ¦™Á‹¦·Â¨³¥n°¥¨µ¥¸ Methyl red ¨³ Congo red ŗo—¸ š¸É š»„‡ªµ¤Á o¤ oœ¸ ´ŠÁ„˜‹µ„nªœÄ¦°Ã‡Ã¨œ¸
„µ¦¥n°¥¨µ¥¸¥o°¤Äœ°µ®µ¦Á®¨ª ¡ªnµ B. subtilis ORB7106 µ¤µ¦™Á‹¦·Â¨³¥n °¥¨µ¥¸¥o°¤Á°ÃŽÅ—o Ĝš»„
£µª³ °Š°»–®£¼¤·Â¨³¡¸Á° ×¥µ¤µ¦™¥n°¥¨µ¥¸ Methyl red ¨³ Congo red ŗo™¹Š 60-98 Áž°¦rÁŽÈœ˜r ĜÁª¨µ
48 ´ÉªÃ¤Š nªœ¸¥o°¤ Azobenzene ¨³ Orange G ¥n°¥¨µ¥Å—oÁ¡¸¥ŠÁ¨È„œo°¥ ‹µ„Ÿ¨„µ¦š—¨°ŠÂ—ŠÄ®oÁ®Èœªn µ ¥¸œ
azoR1 µ¤µ¦™¦oµŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Ášš¸É¥n°¥¨µ¥¸¥o°¤Á°ÃŽÅ—o
‡ÎµÎµ‡´ : „µ¦¥n°¥¨µ¥¸¥o°¤¸Á°ÃŽ Á°ÃŽ¦¸—´„ÁšÂ‡š¸ Á¦¸¥ÂŽ·¨¨´ ´š·¨·
1 £µ‡ª·µª· š¥µ«µ˜¦r ‡–³ª·š¥µ«µ˜¦r¨³Áš‡ÃœÃ¨¥¸ ¤®µª· š¥µ¨´¥Š ¨µœ‡¦· œš¦r ª·š¥µÁ ˜ž{˜˜µœ¸
Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus
* Corresponding author: Ú¦«´¡šr 073-331303 Ú¦µ¦ 073-335130Email-bsukalla@bunga.pn.psu.ac.th,