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‡» –¨´„¬–³Â¨³Ã‡¦Š¦oµŠ °Š¥¸œ Copper chaperone ‹µ„¥µŠ¡µ¦µ (Hevea brasiliensis)
Characterization and Structure of Copper chaperone Gene from Hevea brasiliensis
Áž¨ºÊ°Š »ª¦¦–¤–¸1* ¤«´„—·Í ͇œ»„¼¨1 œª¨¡¦¦– «·¦·œ»¡Š«r 2 ›œ¡´œ›»r ž{šš¤µœœšr1 ‹µ¦»ª´˜¦ ‹´œš¦rž¦³—·¬“r1
£´š¦µª»› ‹·ª˜¦³„¼¨3 ¨³ª´œÁ¡È œ´œšµœ» ª´•œr4
Pluang Suwanmanee1*, Somsak Chocknukul1, Nualpun Sirinupong2, Thanapan Phattamanont1,
Charuvat Chantrapradist 1, Patthavut Jewtragoon 3 and Wanphen Nanthanuwat4
š‡´—¥n °
‹µ„„µ¦Ã‡¨œ ¨³«¹„¬µ‡» –¨´„¬–³ °Š¥¸œ copper chaperone (CCH) ĜœÊε¥µŠ °Š¥µŠ¡µ¦µ (HbCCH )
¡ªnµ¤¸¨Îµ—´Á °Š¥¸œš´ÊŠ®¤— 567 ‡¼nÁ ž¦³„°—oª¥ž¨µ¥ 5 c ¨³ž¨µ¥ 3 c š¸É Ťn™¼„ž¨¦®´ Áž}œ„¦—°³¤·Ãœ
Ášnµ„´ 92 ‡¼n ÁÂ¨³ 217 ‡¼nÁ˜µ¤¨Îµ—´ nªœš¸É™¼„ž¨¦®´ Ášnµ„´ 258 ‡¼n Á‡·—Áž} œ„¦—°³¤·Ãœ 86 ˜´ ª Ž¹ÉŠ¤¸
¤ª¨Ã¤Á¨„»¨Ášnµ„´ 9.2 kDa ¨Îµ—´„¦—°³¤·ÃœÁ®¤º°œ„´ CCH °Š¡º°ºÉœÇ ¼Š¤µ„ ǦŠ¦o µŠ˜˜·¥£¼¤·°ŠÃž¦˜¸œ
HbCCH ž¦³„°—oª¥ 4 E-sheets ¨³ 2 D -helixs ¤¸ metal binding domain (MXCXXC) °¥¼n ¦·Áª– N-terminal
¦³®ªnµŠ E-sheet 1 „´ D-helix 1 ¨³ lysine rich region °¥¼n¦·Áª– C-terminal ¦³®ªnµŠ D-helix 2 „´  E-sheet 4 ¥¸œ
HbCCH ¤¸„µ¦Â—Š°°„ÄœÁœºÊ°Á¥ºÉ°¨Îµ˜oœ „o µœÄ ¨³Ä ¤µ„„ªnµÁœºÊ°Á¥ºÉ°¦µ„
‡ÎµÎµ‡´: Copper chaperone (CCH) ¥µŠ¡µ¦µ
1°µ‹µ¦¥r —¦. £µ‡ª·µ¸ªª· š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´¥š´„¬·– Š ¨µ 90000
Dr., Department of Biology, Faculty of Science, Thaksin University, Songkhla 90000
*corresponding author: e-mail:suwanmaneep@yahoo.com Ú¦«´¡šr 074-311885 ˜n° 2226
2Postdoctoral Biochemistry and Molecular Biology Wayne State University School of Medicine,
540 E. Canfield Avenue Detroit, MI 48201, United States
3 ™µ´œª·‹´¥¥µŠ „¦¤ª·µ„µ¦Á„¬˜¦ °.®µ—Ä®n ‹.Š ¨µ 90110
Songkhla Rubber Research Centre, Hat Yai, Songkhla 90110
4Ÿ¼onª¥ª·‹´ ¥ £µ‡ª·µ¸ªª· š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª· š¥µ¨´¥š´„¬·– Š ¨µ 90000
Research assistants, Department of Biology, Faculty of Science, Thaksin University, Songkhla 90000