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Abstract
The copper chaperone (CCH) gene from latex of Hevea brasiliensis was cloned and characterized. The
Hevea brasiliensis copper chaperone ( HbCCH) cDNA is 567 bp in length, including a 92 bp 5c noncoding region,
a 258 bp open reading frame and 217 bp 3c noncoding region. The coding region of HbCCH represents a putative
86 amino acid residues with a molecular mass of 9.2 kDa. The deduced amino acid sequence of the HbCCH gene
product is highly identical to those of other plant CCHs. Three-dimensional structure of HbCCH contains 4 E-
sheets and 2 D-helixs. Two conserve regions; the N-terminal metal-biding domain (MXCXXC) and lysine rich at
C-terminal were also observed. The N-terminal metal-biding domain was located between E-sheet 1 and D-helix
1 while the lysine rich region was found between D-helix 2 and E-sheet 4. The expression of HbCCH gene in
leaves, petioles and stems was higher than that detected in roots of Hevea seedlings.
Keywords: Copper chaperone (CCH), Hevea brasiliensis
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chaperone) °Š Populus (Accession No. AY603358) Ž¹ÉŠÁž}œ¥¸œ °ŠÃž¦˜¸œ CCH š¸É ¤¸šµšÎµ‡´Äœ„µ¦ œnŠ
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Ä®o„´ copper receptor °Š±°¦räœÁ°›¸¨¸œ Ž¹ÉŠÁž}œ±°¦r 䜡ºš¸É¤¸‡ªµ¤Îµ‡´Äœ„µ¦nŠ´µ– (signal) ޼n
„¨Å„„µ¦˜°œ°Š˜n°‡ªµ¤Á‡¦¸¥— (stress) °Š¡ºÁ¤ºÉ °Á„·—„µ¦‡»„‡µ¤ °ŠÁºÊ°Ã¦‡ ®¦º°Á„·—µ—Ÿ¨ Áž} œ˜oœ
(Company and Gonzalez-Bosch, 2003) œ°„‹µ„œ¸ÊÞ¦˜¸œ CCH ¥´Š¤¸ šµšÎµ‡´Äœ„µ¦‡ª‡» ¤¤—»¨ °Š›µ˜»
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™¼„„¦¸ —Ĝ˜n¨³‡¦´ÊŠ ‹³¤¸ Ÿ¨˜n°‡ªµ¤Á‡¦¸ ¥—¨³¤—»¨ °Š›µ˜» š°ŠÂ—ŠÄœ˜oœ¥µŠ¡µ¦µ šÎ µÄ®o˜oœ¥µŠÄ®oŸ¨Ÿ¨·˜
¨—¨ŠÂ¨³°µ‹šÎ µÄ®o Á„·—懮¦º°°µ„µ¦Ÿ· —ž„˜·„´˜oœ¥µŠ Ánœ °µ„µ¦Áž¨º°„®oŠ Áž} œ˜oœ Šµœª·‹´ ¥œ¸ÊŗoǨœ¥¸ œ
HbCCH ‹µ„¥µŠ¡µ¦µ «¹„¬µ‡» –¤´˜·Â¨³Ã‡¦Š¦oµŠ˜˜·¥£¼¤·°Š¥¸œ Ž¹É Š o°¤¼¨š¸Éŗo ¦´¤¸ž¦³Ã¥œr˜n°„µ¦¡´•œµ¥¸œ
HbCCH ޞ¦³¥»„˜rčoĜ„µ¦‹´—„µ¦Á„¸É¥ª„´„µ¦„¦¸—¥µŠÁ¡ºÉ°Ä®oŗo Ÿ¨Ÿ¨·˜š¸ÉÁ®¤µ³¤Ã—¥Å¤n¤¸Ÿ¨„¦³š˜n°˜o œ¥µŠ
‹œ„¦³š´ÉŠœÎµÅž¼n„µ¦Á„·—懮¦º °°µ„µ¦Áž¨º°„®oŠ˜n°Åž