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ž{ ‹‹»´œ¤¸„µ¦œÎµ¸¥o°¤Á°ÃŽ (Azo dye) ¤µÄo°¥n µŠÂ¡¦n®¨µ¥Äœ°»˜µ®„¦¦¤˜nµŠÇ Ánœ °»˜µ®„¦¦¤·ÉŠš°
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„µ¦˜„˜³„°œ—o ª¥µ¦Á‡¤¸ „µ¦Â¨„Áž¨¸É¥œÅ°°°œ „µ¦Äo ðΜ ˜nª·›¸Î µ´——´Š„¨nµª ¤¸o°‹Îµ„´—®¨µ¥ž¦³„µ¦ Ánœ
‡nµÄo ‹nµ¥Äœ„µ¦Îµ´—‡n °œ oµŠ¼Š ˜³„°œš¸É ŗo‹µ„„µ¦Î µ´—„È ¤¸Áž}œ‹Îµœªœ¤µ„ ¨³¤¸ µ¦¡·¬˜„‡oµŠ ¥µ„˜n°„µ¦
„ε‹´—˜n°Åž (Sen and Demirer, 2003) —´ Šœ´Êœª·›¸„µ¦Îµ´ —šµŠ¸ª£µ¡ ‹¹ŠÁž}œšµŠÁ¨º°„š¸É—¸šµŠ®œ¹ÉŠ š¸É µ¤µ¦™Îµ´—¸
¥o°¤Å—o ¼ŠÂ¨³‡nµÄo‹n µ¥œo°¥„ªnµ (Yang et al. , 2003) ª·›¸„µ¦Îµ´—šµŠ¸ ªª·š¥µ Áž} œª·›¸ „µ¦š¸É°µ«´ ¥‡ªµ¤µ¤µ¦™ °Š
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Á‡¥¤¸ „µ¦«¹ „¬µ ¡ªnµ ‹»¨·œš¦¸¥rÁ®¨nµœ¸Ê µ¤µ¦™¦oµŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš š¸É µ¤µ¦™¥n °¥¨µ¥¡´œ›³Á°ÃŽÅ—o
(Bin et al., 2004; Deng et al ., 2008) —oª¥Á®˜» œ¸Ê‹¹ŠšÎµÄ®oŠµœª·‹´¥®¨µ¥Ç Á¦ºÉ °Š¤»nŠÁœoœÄœ„µ¦«¹„¬µÂ¨³®µœ· — °Š
‹»¨·œš¦¸¥rš¸É ¤¸‡ªµ¤µ¤µ¦™Äœ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽ Á¡ºÉ°œÎµ¤µž¦³¥»„˜rčo˜n°Åž ‹µ„Šµœª·‹´¥ °Š Leelakriangsak
et al. (2008) ¨³ Töwe et al. (2007) ŗo«¹„¬µÂ‡š¸Á¦¸ ¥ B. subtilis ¨³¡ªnµ B. subtilis ¤¸¥¸œ azoR1 ¨³ azoR2
Ž¹ÉŠ™¼„‡ª‡» ¤„µ¦Â—Š°°„ץÞ¦˜¸ œ YodB ¨³ MhqR ‹µ„ Swiss-Prot database ¥¸œ azoR1 ¨³ azoR2
´ŠÁ‡¦µ³®rÞ¦˜¸œš¸É¤¸¨Îµ—´„¦—°³¤·Ãœ‡¨oµ¥‡¨¹ Š„´ FMN-dependent NADH-azoreductase Ĝ„µ¦¦oµŠÁ°œÅŽ¤r
Á°ÃŽ¦¸—´„Ášš¸É µ¤µ¦™¥n °¥¨µ¥¡´œ›³ °Š¸¥o°¤Á°ÃŽÅ—o œ°„‹µ„œ¸Ê Nishiya ¨³ Yamamoto (2007) ŗo«¹„¬µ
‡»–¤´˜·°ŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš‹µ„¥¸ œ azoR2 ×¥„µ¦„´—¨³šÎµÄ®o ¦· » š›·Í ‹µ„„µ¦«¹„¬µ¡ªnµ Á°œÅŽ¤r
Á°ÃŽ¦¸—´„Áš ‹µ„¥¸œ azoR2 ¤¸ž¦³·š›·£µ¡Äœ„µ¦¥n°¥¨µ¥¸ ¥o°¤Á°ÃŽœ·— Methyl red ˜n ¥´ŠÅ¤n¤¸¦µ¥Šµœ
„µ¦«¹„¬µ‡ªµ¤µ¤µ¦™ °ŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš‹µ„ ¥¸œ azoR1 Ĝ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽ ‹¹ŠšÎµÄ®o¤¸‡ªµ¤œÄ‹
«¹„¬µÂ‡š¸Á¦¸¥Â„¦¤ª„ œ·— B. subtilis š¸É ¤¸¥¸œ azoR1 Ĝ„µ¦¥n °¥¨µ¥¸¥o°¤Á°ÃŽ ×¥µ¥¡´œ›»rš¸ÉœÎ µ¤µ«¹„¬µ
Áž}œ B. subtilis µ¥¡´ œ›»r ORB7106 (Töwe et al. 2007) š¸É¤¸„µ¦„¨µ¥ °Š¥¸œ yodB šÎµÄ®o¤¸„µ¦Â—Š°°„ °Š¥¸ œ
azoR1 ¤µ„™¹Š 70 Ášnµ Á¤ºÉ °Áš¸¥„´ B. subtilis µ¥¡´ œ›»r JH642 (Wild type) (Leelakriangsak et al., 2008)
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Šµœª·‹´¥‡¦´Ê Šœ¸ÊŗošÎµ„µ¦«¹„¬µ‡ªµ¤µ¤µ¦™ °Š B. subtilis ORB7106 (azoR1-lacZ, 'yodB, tetr, cmr ) š¸É¤¸
„µ¦¦o µŠÁ°œÅŽ¤rÁ°ÃŽ¦¸—´„Áš (AzoR1) Ĝ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽœ·— Azobenzene, Methyl red, Orange G ¨³
Congo red š¸É‡ªµ¤Á o ¤ oœÁ¦·É¤˜oœ 10, 50, 100 ¨³ 200 ¤·¨¨·„¦´ ¤˜n°¨·˜¦ ×¥«¹„¬µ„µ¦¥n°¥¨µ¥¸ ¥o°¤Äœ£µª³š¸É
˜„˜n µŠ„´œ °Š°»–®£¼¤· ˜´ÊŠÂ˜n 25, 30, 37, 40 ¨³ 45 °Š«µÁŽ¨ÁŽ¸¥Â¨³ š¸É¡¸Á° 5, 7 ¨³ 9 ¨³šÎµ„µ¦«¹„¬µ
Áž¦¸ ¥Áš¸¥‡ªµ¤µ¤µ¦™Äœ„µ¦¥n°¥¨µ¥¸¥o°¤Á°ÃŽÄœ°µ®µ¦Á®¨ª„´ B. subtilis JH642 (wild type)