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Ÿ¨ °Š„µ¦„¦¸—˜n°„µ¦Â—Š°°„ °Š TDFREP241 Ĝ¥µŠ¡µ¦µµ¥¡´œ›»r RRIC110
‹µ„„µ¦ª·Á‡¦µ³®r„µ¦Â—Š°°„ °Š TDFREP241 ĜœÊÎ µ¥µŠ °Š¥µŠ¡µ¦µ„¨»n¤š¸É„¦¸—™¸É „´„¨»n¤„¦¸—ž„˜·Áž}œ
Áª¨µ 10 ª´œ ¡ªnµª´œš¸É 1 ‡nµ„µ¦Â—Š°°„ °Š¥¸œÄœ„¨»n ¤š¸É„¦¸—™¸É „´„¨»n¤„¦¸—ž„˜·Â˜„˜n µŠ„´œ°¥nµŠÅ¤n¤¸œ´¥Îµ‡´
šµŠ™·˜·š¸É¦³—´ 0.05 nªœª´œš¸É 4, 7 ¨³ 10 ‡nµ„µ¦Â—Š°°„ °Š¥¸œÄœ„¨»n ¤š¸É„¦¸ —™¸É ¼Š„ªnµ„¨»n ¤„¦¸—ž„˜· °¥nµŠ¤¸
œ´¥Îµ‡´šµŠ™·˜·š¸É¦³—´ 0.05 Á¤ºÉ°Áž¦¸ ¥Áš¸¥£µ¥Äœ„¨»n¤Á—¸¥ª„´ œ¡ªnµ„¨»n¤š¸É „¦¸—™¸É‡nµ„µ¦Â—Š°°„ °Š¥¸œÄœÂ˜n
¨³ª´œÂ˜„˜nµŠ„´œ°¥nµŠ¤¸œ´ ¥Îµ‡´šµŠ™·˜·š¸É ¦³—´ 0.05 ×¥ª´œš¸É 1 ¤¸„µ¦Â—Š°°„ °Š¥¸œ˜Éε»—Â¨³Á¡·É ¤ ¹Êœ¼Š»—
Ĝª´œš¸É 4 ‹µ„œ´Ê œ‹¹Š¨—¨ŠÄœª´œš¸É 7 ¨³ 10 ˜µ¤¨Îµ—´ nªœ„¨»n¤š¸É„¦¸—ž„˜· ‡nµ„µ¦Â—Š°°„ °Š¥¸œÄœÂ˜n¨³ª´œ
˜„˜nµŠ„´ œ°¥nµŠÅ¤n¤¸œ´¥Î µ‡´šµŠ™·˜·š¸É ¦³—´ 0.05 (£µ¡š¸É 3) —Šªnµ„µ¦„¦¸—™¸ÉšÎ µÄ®o˜oœ¥µŠÁ„· —‡ªµ¤Á‡¦¸¥—¼Š
„ªn µ„µ¦„¦¸—ž„˜· ÁœºÉ °Š‹µ„„µ¦„¦¸—˜n¨³‡¦´Ê Š¤¸Ÿ¨šÎµÄ®oÁ„·— oxidative stress „µ¦¼Á¸¥œÊ娳›µ˜» °µ®µ¦˜nµŠÇ
°Š˜oœ¥µŠ
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1
1.5
2
2.5
3
3.5
4
Relative expression levels
£µ¡š¸É 3 „µ¦šÎµ°·Á¨‡Ãš¦Ã¢¦¸Ž· RT-PCR product °Š TDFREP241 ¨³ 18S rRNA ĜÁ‹¨°³„µÃ¦‡ªµ¤Á o ¤ oœ
2 Áž°¦rÁŽÈ œ˜r („) ¨³‡nµÁŒ¨¸É¥„µ¦Â—Š°°„ °Š TDFREP241 ( ) ĜœÊε¥µŠ °Š˜oœ¥µŠš¸É„¦¸ —™¸É (frequency tapping =
F) ¨³„¦¸—ž„˜· (normal tapping = N) Áž}œÁª¨µ 10 ª´œ
18S rRNA
TDFREP241
F N F N F N F N
ª´œš¸É 1 ª´œš¸É 4 ª´œš¸É 7 ª´œš¸É 10
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