เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 1238

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¨³œÎµµ¦„´—ž‡˜·œš¸Éŗo¤µÄoĜ„µ¦„µ¦¨—œÊ宜´„¨³Ã‡Á¨Á˜°¦°¨Äœ´˜ªrš—¨°Š ×¥«¹ „¬µ‡nµ BMI (Body
Mass Index), °»–®£¼¤·¦nµŠ„µ¥ (body temperature), „µ¦Äo‡¨¨°¦¸É (calorie expenditure) ¨³Å ¤´œ³¤ (adipose
tissue) Ĝ´˜ªrš—¨°Š
„µ¦šÎµµ¦„´—Áž‡˜·œÄœ¦¼žÂÁ¤È — (pellet) ¤¸ nªœŸ¤—´Š¼˜¦˜Îµ¦´˜n°Åžœ¸Ê šÎµ°µ®µ¦Á¤È—Å—o
25,000 Á¤È— ‹³Å—o°µ®µ¦¦¼žÂÁ¤È—°´ —¡·¤¡rš¸É¤¸Áž‡˜·œÁž}œnªœž¦³„°ž¦³¤µ– 0.4 „¦´¤˜n°Á¤È—
RX Corn Starch 300.0 g.
Caseine 100.0 g.
Lactose 100.0 g.
Powdered sucrose 100.0 g.
Pectin ad to 1,000.0 g.
Water q.s.
„µ¦«¹„¬µŸ¨ °ŠÁž‡˜·œ‹µ„¤³œµª˜n°‡nµ BMI ×¥œÎµ®œ¼™¸‹´„¦(Mice) ˜´ ªÁ¤¸¥‹Îµœªœ 10 ˜´ª ¤µ´ÉŠ
œÊÎ µ®œ´„ ¨³‡ªµ¤¥µª˜´ª (ª´ —‹µ„ž¨µ¥‹¤¼„™¹Š„oœÅ¤n ¦ª¤‡ªµ¤¥µª®µŠ) „n°œ„µ¦š—¨°Š ×¥™º°Áž} œª´œš¸É 0 ‹µ„œ´Ê œ
Ä®o®œ¼ „·œµ¦„´—Áž‡˜·œÄœ¦¼ žÂÁ¤È— ž¦· ¤µ– 10 „¦´¤/ª´œ/˜´ª ¦³®ªnµŠÁª¨µ 11.00 œ.š»„ª´œ Áž}œÁª¨µ 45 ª´œ.´ÉŠ
œÊÎ µ®œ´„˜´ªÂ¨³‡ªµ¤¥µª˜´ª °Š®œ¼®¨´Š‹µ„®œ¼ „·œµ¦„´—Áž‡˜·œÅžÂ¨oª 1 ´ÉªÃ¤Š ž¦³¤µ–Áª¨µ 12.00 œ. š»„ª´œ
‡Îµœª–‡nµ BMI °Š®œ¼ š»„ 15 ª´œ ×¥‡Îµœª–—´ Šœ¸Ê
‡nµ BMI = œÊ宜´„Áž}œ „·Ã¨„¦´¤
(‡ªµ¤¥µª˜´ªÁž}œÁ¤˜¦)2
„µ¦«¹„¬µŸ¨ °ŠÁž‡˜·œ‹µ„¤³œµª˜n°°»–®£¼¤·¦nµŠ„µ¥ Ÿ¨˜n °‡nµ‡°Á¨Á˜°¦°¨ ¨³Ÿ¨˜n° adipose
tissue °Š®œ¼ ™¸‹´ „¦ —´—ž¨Š‹µ„„µ¦š—¨°Š °Š Hayashi, et al (2004), Calapai, et al (1999) ¨³ °Š Maeda, et
al (2005) ˜µ¤¨Îµ—´ ץčo ®œ¼™¸‹´ „¦Á¡«Á¤¸¥ 2 „¨»n¤ „¨»n¤¨³ 10 ˜´ª „¨»n¤š¸É 1 Áž} œ„¨»n¤‡ª‡»¤ Ťn Ä®o„·œµ¦Ä—
œ°„‹µ„°µ®µ¦ž„˜· „¨»n ¤š¸É 2 Áž}œ„¨»n¤š¸É Ä®o„·œ°µ®µ¦ž„˜· ¨³µ¦„´ —Áž‡˜·œÄœ¦¼ žÂÁ¤È— (pellet)ž¦·¤µ– 10
„¦´ ¤/ª´œ/˜´ª ¦³®ªnµŠÁª¨µ 11.00 œ.š»„ª´ œ ª´—°» –®£¼¤·°Š¦nµŠ„µ¥Ã—¥Äo rectal thermometer probe for animal °—
Á oµšµŠšªµ¦®œ´ „ °Š®œ¼Ä®o¨¹„ž¦³¤µ– 2 ÁŽœ˜·Á¤˜¦ ¨³¦°‹œ°»–®£¼¤·‡Šš¸É ‹¹Š°n µœ‡nµ „µ¦ª´—°»–®£¼¤· ×¥ª´ —š´ÊŠ
„n°œÂ¨³®¨´ŠÄ®o µ¦Â¨oªž¦³¤µ– 1 ´ÉªÃ¤Š š»„ 3 ª´ œÁž}œÁª¨µ 30 ª´ œ ®¨´Š‹µ„Á¨¸Ê¥Š®œ¼Áž}œÁª¨µ 1 Á—º°œ Á¨º°— °Š
®œ¼™¼„Â¥„°°„¤µ‹µ„ÁoœÁ¨º°—®´ªÄ‹ (heart puncture) ¨³œÎµÅž˜¦ª‹ª´—ÇÁ¨Á˜°¦°¨Äœ whole serum ×¥ª·›¸
CHOL (CHOD-PAP method) —oª¥ emzymatic kit (Boehringer-Manheim, Germany). ¨³˜¦ª‹®µ Total
triacylglycerol ץčo GPO Trigluceride kit (Randox Laboratories,USA.) ®œ¼‹³™¼„‰nµÃ—¥ª· ›¸ cervical dislocation
¨³Â¥„ white adipose tissue ®œoµn°Ššo°Š °Š®œ¼ ¦ª¤š´Ê Š brown adipose tissue °¥nµŠ¦ª—Á¦Èªš´œš¸ ´ÉŠœÊ宜´„ °Š
adipose tissue š´ÊŠ°Š