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7 µ¦Î
µ SDS-PAGE
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E. coli
BL21 plus (DE3) RIL uninduced
(un) ¨³ induced (in) o
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0.1 mM °»
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25
q
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Áª¨µ 12 ´É
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Broad Range
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E. coli
BL21 plus (DE3) RIL uninduced
(un) ¨³ induced (in) o
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o
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o
0.1 mM °»
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37
q
C Á}
Áª¨µ 3 ´É
ªÃ¤ (in-3) ¨³ 6 ´É
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(in-6); M = protein marker Broad Range
34.6 kDa
27 kDa
Soluble
Pellet
M un in-3 in- 6 in-3
in-6
cell soluble pellet
M un in in in
soluble pellet
27 kDa
36.4 kDa
pSUMO-
Hb
REF