เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 323

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥ ¤®µª·š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
2
microorganism in the assimilation of waste lubricating oil and Bio-emulsifier production was isolate PO1.2. The
isolated yeast strain, PO 1.2 was characterized on YM agar plate as followed: growth at 30qC, off-white color,
smooth with margins ranging from smooth to lobed, and flat. Determination of the nucleotide sequence of the gene
encoding 26S rDNA of the isolate PO1.2 was identified as Issatchenkia orientalis. I. orientalis PO1.2 showed the
best degrading activity was 63.60% when waste lubricating oil was 0.5%. In addition, I. orientalis PO1.2 can
produce bio-emulsifier. Determination of emulsification activity (%EA) from I. orientalis PO1.2 with soy bean oil,
palm oil or rice bran oil, the result showed the best %EA with rice bran oil (71.67%) when used 0.1% waste
lubricating oil as a carbon source.
Keyword: Bio-emulsifier/waste lubricating oil-degrading microorganisms/waste lubricating oil/yeast
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