เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 333

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥ ¤®µª·š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
Abstract
The chemical compositions of wild and farmed catfish in skin and muscle portions were analyzed.
Pla-duk-ra had higher lipid, protein and ash contents than raw catfish. However, the farmed catfish had higher
lipid content than wild catfish (Pd0.05). Both catfish showed marked increases in free fatty acids, with decreases
in triglyceride and phospholipid content after drying and fermentation (Pd0.05). This suggested that hydrolysis
was induced by lipases and phospholipases. Decreases in polyunsaturated fatty acids, especially eicosapentaenoic
acid (EPA) and docosahexaenoic acid (DHA), were observed. Drying and fermentation caused the changes of
chemical composition, lipolysis and lipid oxidation of catfish muscle and skin portions. Both catfish muscle had
myofibrillar proteins as the major protein (50.44-55.04%) followed by sarcoplasmic protein (29.23-29.90%),
alkali soluble protein (13.64-16.17%) and stroma protein (2.07-3.49%). Sodium dodecylsulfate-polyacrylamide
gel electrophoresis (SDS-PAGE) analysis revealed that myosin heavy chain (MHC) and actin were the major
proteins in both species. After drying and fermentation of pla-duk-ra, MHC and actin were decreased. These data
indicated that the myofibrila proteins of both catfish were hydrolyzed during processing.
Keywords: dry fermented catfish (Pla-duk-ra), drying, fermentation, chemical composition, lipid and protein
‡Î µœÎµ
ž¨µ—»„¦o µÁž}œŸ¨·˜£´–”r°µ®µ¦¡ºÊ œÁ¤º°Šš¸ÉÁž}œš¸Éœ· ¥¤¦·Ã£‡Â¨³¤¸ºÉ°Á¸ ¥ŠÄœ‹´Š®ª´ —¡´š¨»Š œ°„‹µ„œ¸Ê¥´Š
Áž}œ·œ‡oµ®œ¹ÉŠ˜Îµ¨®œ¹ÉŠŸ¨·˜£´–”r (OTOP) š¸ÉšÎ µ¦µ¥Å—o„nž¦³µœÅ—o®¨µ¥·¨oµœµš˜n°že ®¨nŠŸ¨·˜ž¨µ—» „
¦oµš¸É ε‡´‡º° ¡ºÊœš¸É¦·Áª–¦° Ç š³Á¨œo°¥ °ÎµÁ£°‡ªœ œ»œ ‹´Š®ª´—¡´š¨»Š µªš³Á¨œo°¥¤¸«´„¥£µ¡Äœ„µ¦Ÿ¨· ˜ž¨µ
—»„¦oµÅ—o ¼Š„ªn µÂ®¨nŠŸ¨·˜°ºÉ œ Ç ÁœºÉ°Š‹µ„¤¸™·Éœ°µ«´¥°¥¼nĜ¦·Áª–®¨n ŠœÊε‹º—Ž¹É Š°»—¤Åž—oª¥ž¨µœÊÎ µ‹º—œµœµœ· —
¦ª¤š´ÊŠ¦¦¡»¦»¬ °Šµªš³Á¨œo°¥Å—o ´ÉŠ¤£¼¤·ž{µÄœ„µ¦Âž¦¦¼žž¨µœÊε‹º—¤µoµœµœ šÎµÄ®oµªš³Á¨œo°¥¤¸ ¼˜¦
¨³„µ¦Ÿ¨·˜š¸ÉšÎ µÄ®ož¨µ—»„¦oµ¤¸¦µ˜·—¸Áž}œš¸É¥°¤¦´  °ŠŸ¼o¦·Ã£‡„´œ°¥nµŠÂ¡¦n ®¨µ¥ ž¨µ—»„š¸Éčo 宦´„µ¦Ÿ¨· ˜
ž¨µ—»„¦oµ¤¸ °Šœ·—‡º ° ž¨µ—»„°» ¥‹µ„›¦¦¤µ˜· ( Clarias macrocephalus) ¨³ž¨µ—» „Á¨¸Ê¥ŠŽ¹ÉŠÁž}œž¨µ—» „¨¼„Ÿ¤
¦³®ªnµŠž¨µ—» „°»¥Â¨³ž¨µ—»„Áš« (Clarias gariepinus) Á„¬˜¦„¦Á¦¸¥„ž¨µ—» „¨¼„Ÿ¤œ¸Êªnµž¨µ—»„·Ë „°»¥ (Clarias
macrocephalus ¯Clarias gariepinus) ÁœºÉ°Š‹µ„ž{®µ„µ¦Á¡·É¤‹Îµœªœž¦³µ„¦Â¨³ž{®µ£µª³Âª—¨o°¤Áž}œ¡·¬
nŠŸ¨Ä®o ž¨µ—»„°»¥ ‹µ„®¨nŠœÊ囦¦¤µ˜· ¤¸ž¦·¤µ–œo°¥¨Š¤µ„ ‹¹ ŠnŠŸ¨Ä®o ž¨µ—»„°» ¥‹µ„›¦¦¤µ˜·®µÅ—o¥µ„¨³¤¸
¦µ‡µÂ¡Š Ĝž{ ‹‹»´œž¨µ—»„Á¨¸Ê¥Š‹¹Š¤¸šµšÎµ‡´Äœ„µ¦Ÿ¨·˜ž¨µ—» „¦oµÁ¡ºÉ°„µ¦‡oµ ˜n°¥n µŠÅ¦„Șµ¤Ÿ¨·˜£´–”rž¨µ
—»„¦o µš¸ÉŸ¨·˜‹µ„ž¨µ—»„Á¨¸Ê¥Š¥´ Š‡Š¤¸‡»–£µ¡š´ÊŠšµŠ—o µœÁ‡¤¸ „µ¥£µ¡ ž¦³µš´¤Ÿ´ ¨³°µ¥»„µ¦Á„ȝ¦´„¬µ Áž}œš¸É
¥°¤¦´ œo°¥„ªnµž¨µ—»„¦oµš¸É Ÿ¨·˜‹µ„ž¨µ—»„°»¥›¦¦¤µ˜· š´ÊŠœ¸Ê°µ‹ÁœºÉ°Š¤µ‹µ„ž¨µ—»„š´ÊŠ°Šœ·—œ¸Ê¤¸°Š‡rž¦³„°
šµŠÁ‡¤¸š¸É ÁÁ˜„˜nµŠ„´œ —´ Šœ´Êœ„µ¦«¹ „¬µŸ¨ °Š„µ¦Âž¦¦¼žž¨µ—»„¦o µÃ—¥®¤´„¨³„µ¦šÎ µÂ®oŠ˜n°„µ¦Áž¨¸É¥œÂž¨Š °Š
Å ¤´ œÂ¨³Ãž¦˜¸œÄœ„¨oµ¤ÁœºÊ°ž¨µ—» „Á¨¸Ê¥ŠÂ¨³ž¨µ—» „›¦¦¤µ˜· ‹¹ŠÁž}œž¦³Ã¥œr°¥n µŠ¥·ÉŠ˜n °„µ¦¥„¦³—´‡»–£µ¡
°Šž¨µ—»„¦o µš¸ÉŸ¨· ˜‹µ„ž¨µ—»„Á¨¸Ê¥ŠÄ®o °¥¼nĜ¦³—´Á—¸ ¥ª„´ž¨µ—» „›¦¦¤µ˜· œ°„‹µ„œ¸Ê o°¤¼¨š¸Éŗo ‹µ„„µ¦ª· ‹´¥¥´Š