เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 828

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´„¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
„µ¦ª·Á‡¦µ³®r ž¦·¤µ–µ¦ž¦³„°¢eœ°¨·„š´ÊŠ®¤— (Total phenolic content)
„µ¦ª· Á‡¦µ³®rž¦·¤µ–µ¦ž¦³„°¢eœ°¨·„š´ÊŠ®¤—čoª·›¸ „µ¦ °Š Singleton ¨³ Rossi (1965) ×¥œÎµµ¦
„´—‹µ„Áž¨º °„‡ªµ¤Á o¤ oœ 0.5 ¤· ¨¨·„¦´¤/¤· ¨¨·¨·˜¦ ž¦· ¤µ˜¦ 200 ŤÇ¦¨·˜¦ Än ¨ŠÄœ®¨°—š—¨°Š œµ—
10 ¤·¨¨·¨·˜¦ Á˜·¤ Folin-Ciocalteu ž¦·¤µ˜¦ 1.5 ¤·¨¨·¨·˜¦ š·Ê ŠÅªoš¸É °»–®£¼¤·®o °Š œµœ 5 œµš¸ Á˜·¤µ¦¨³¨µ¥ÃŽÁ—¸¥¤
ŝ‡µ¦r °Áœ˜ ‡ªµ¤Á o¤ oœ 0.566 䨵¦r ž¦·¤µ˜¦ 1.5 ¤·¨¨·¨·˜¦ Á ¥nµµ¦¨³¨µ¥Ä®oÁ o µ„´œ š·ÊŠÅªo š¸É°»–®£¼¤·®o °Š œµœ
90 œµš¸ ª´ —‡nµ„µ¦—¼—„¨º œÂŠš¸É 725 œµÃœÁ¤˜¦ —oª¥Á‡¦ºÉ°Š UV/Vis spectrophotometer ‡Îµœª–ž¦·¤µ–
µ¦ž¦³„°¢eœ°¨¨·„š´ÊŠ®¤— ×¥„µ¦Áž¦¸¥Áš¸¥„´„¦µ¢¤µ˜¦“µœ °Šµ¦¨³¨µ¥ furulic acid
„µ¦ª·Á‡¦µ³®r ž¦·¤µ– Reducing power
„µ¦ª· Á‡¦µ³®rž¦·¤µ– reducing power čoª·›¸„µ¦ °Š Oyaizu (1996) ×¥œÎµµ¦„´—‹µ„Áž¨º °„ oµª‡ªµ¤
Á o¤ oœ 0.5 ¤·¨¨·„¦´ ¤/¤·¨¨·¨·˜¦ ž¦· ¤µ˜¦ 2.5 ¤·¨¨·¨·˜¦ Än¨ŠÄœ®¨°—š—¨°Š œµ— 25 ¤· ¨¨·¨·˜¦ Á˜·¤ 0.2 䨵¦r
¢°Á¢˜´Á¢°¦r ¡¸ Á° 6.6 ž¦·¤µ˜¦ 2.5 ¤·¨¨·¨·˜¦ Á˜·¤µ¦¨³¨µ¥ÃžÂšÁŽ¸¥¤ÅŽ¥µÅœ—r 1% ž¦·¤µ˜¦ 2.5 ¤·¨¨·¨·˜¦
n ¤Äœ°nµŠœÊε¦o°œ (water bath) š¸É°»–®£¼¤· 50 °Š«µÁŽ¨ÁŽ¸¥œµœ 20 œµš¸ Á˜· ¤µ¦¨³¨µ¥„¦—ئ‡¨°Ã¦°³Ž·˜·„
‡ªµ¤Á o¤ oœ 10% ž¦·¤µ˜¦ 2.5 ¤· ¨¨·¨·˜¦ œÎµÅžž{~œÁ®ª¸É¥Š (centrifuge) š¸É 650g œµœ 10 œµš¸ œÎµn ªœÄž¦·¤µ˜¦
5 ¤· ¨¨·¨·˜¦ Än¨ŠÄœ®¨°—š—¨°Š œµ— 25 ¤· ¨¨·¨·˜¦ Á˜·¤œÊÎ µ„¨´É œ ž¦·¤µ˜¦ 5 ¤·¨¨·¨·˜¦Á˜·¤µ¦¨³¨µ¥Á¢°¦r ¦·‡
‡¨°Å¦—r‡ªµ¤Á o¤ oœ 0.1% ž¦·¤µ˜¦ 1 ¤·¨¨·¨·˜¦ Á ¥nµµ¦¨³¨µ¥Ä®oÁ oµ„´œ ª´—‡nµ„µ¦—¼—„¨ºœÂŠš¸É 700 œµÃœÁ¤˜¦
—oª¥Á‡¦ºÉ°Š UV/Vis spectrophotometer ‡Îµœª–ž¦·¤µ– reducing power ×¥„µ¦Áž¦¸¥Áš¸¥„´„¦µ¢¤µ˜¦“µœ °Š
µ¦¨³¨µ¥ furulic acid
„µ¦ª·Á‡¦µ³®r Radical scavenging activity ×¥ª·›¸ DPPH system
„·‹„¦¦¤„µ¦˜oµœ°°„Ž·Á—´œÄœ¦¼ž °Š radical scavenging activity čo ®¨´„„µ¦ª´—‡n µ—¼—„¨ºœÂŠ °Š
DPPH š¸É Áž¨¸É¥œÂž¨ŠÅž (Hatano ¨³‡–³, 1988) ×¥œÎµµ¦„´—‹µ„Áž¨º°„ oµª‡ªµ¤Á o¤ oœ 0.5 ¤·¨¨·„¦´ ¤˜n°
¤·¨¨·¨·˜¦ ž¦· ¤µ˜¦ 0.1 ¤·¨¨·¨·˜¦ Än¨ŠÄœ®¨°—š—¨°Š œµ— 10 ¤·¨¨· ¨·˜¦ Á˜· ¤µ¦¨³¨µ¥ DPPH (2,3-diphenyl-1-
picrylhydrazyl) ‡ªµ¤Á o¤ oœ 6 x 10-5 䨵¦rĜµ¦¨³¨µ¥Á¤šµœ°¨ ž¦·¤µ˜¦ 3.9 ¤·¨¨·¨·˜¦ Á ¥nµµ¦¨³¨µ¥Ä®oÁ oµ
„´œ ˜´ÊŠš·ÊŠÅªoĜš¸É¤º—œµœ 30 œµš¸ ª´—‡nµ„µ¦—¼—„¨ºœÂŠ °Š˜´ª°¥nµŠÂ¨³ blank š¸É‡ªµ¤¥µª‡¨ºÉœ 517 œµÃœÁ¤˜¦ —oª¥
Á‡¦ºÉ°Š UV/Vis spectrophotometer ‡Îµœª–®µ % °Š radical scavenging activity ˜µ¤¤„µ¦
DPPH-scavenging activity (%) = (OD 517 »—‡ª‡»¤ - OD517 ˜´ª°¥n µŠ) X 100
(OD517 »—‡ª‡»¤)
Ÿ¨„µ¦ª·‹´¥Â¨³°£·ž¦µ¥Ÿ¨
ž¦·¤µ–µ¦„´—‹µ„Áž¨º°„ oµª
ž¦· ¤µ–µ¦„´—®¥µ‹µ„Áž¨º °„ o µª (Rice Hull) 5 ¡´œ›»r ‡º° ¡´œ›»r ÁŒ¸Ê¥Š¡´š¨» Š ¡´œ›»rÁ¨È œ„ž{˜˜µœ¸
¡´ œ›»r ´Š r®¥— ¡´œ›»r‹oµª¡´š¨»Š ¨³¡´œ›»rÁ È ¤š°Š¡´š¨»Š š¸É „´——oª¥—oª¥ 50 ¨³ 70% Á°šµœ°¨ —ŠÄœ˜µ¦µŠš¸É 1
¡ªnµž¦·¤µ–µ¦„´ —®¥µš¸É „´—Å—o‹µ„˜´ ªšÎµ¨³¨µ¥ 70% Á°šµœ°¨¤¸ž¦·¤µ–¼Š„ªnµ„µ¦„´——oª¥ 50% Á°šµœ°¨