full2010.pdf - page 1125

1087
A
0
20
40
60
80
100
0 6 12 18 24 30 day(s)
% Amp resistant colony
pHY300PLK
pHYB43
pBluescript II KS
pCHIB1
¦¼ žš¸É 1 „µ¦‡Š°¥¼n °Š¡¨µ¤·—Äœ Escherichia coli DH5v (A) ®¦º° INV vF c (B) ×¥‡Îµœª–°°„¤µÁž}œ‡nµÁž°¦rÁŽÈ œ˜r„µ¦—ºÊ°¥µ
°¤¡·Ž·¨¨·œ.
‹µ„„µ¦«¹ „¬µ segregrational stability °Š¡¨µ¤·—œ·—˜nµŠÇ Ĝ E. coli DH5v ®¦º° INVvFc ×¥„µ¦œÎµ
ÁºÊ°š¸É¤¸¡¨µ¤·—ÅžÁ¨¸Ê¥ŠÄœ£µª³ non selective pressure °¥nµŠ˜n°ÁœºÉ °ŠÁž}œÁª¨µ 30 ª´œ ¨oªœÎµÁºÊ°ÄœÂ˜n ¨³ª´œ¤µ
„¦³‹µ¥¨Šœ‹µœÁ¡µ³ÁºÊ° LB š¸É¤¸Â¨³Å¤n¤¸¥µž’·¸ªœ³Â°¤¡·Ž· ¨¨·œ Á¡ºÉ°˜¦ª‹œ´ ‹ÎµœªœÃ‡Ã¨œ¸Â¨³‡Îµœª–
B
0
20
40
60
80
100
0 6 12 18 24 30
day(s)
%Amp resistant colony
pHY300PLK
pHYB43
pBluescript II KS
pCHIB1