1084
µ¦µ¸É
1 µ¥¡´
»r
第r
Áo
µo
µ (host strains) ¨³¡¨µ¤·
¸É
Äo
ĵ¦«¹
µ
Strains or plasmid
Relevant properties
Source or reference
Host strains
E. coli
DH5
v
sup
E44
'
lac
U169 (f 80
lac
Z
'
15)
hsd
R17
rec
A1
end
A1
gyr
A96
thi
-1
rel
A1
Gibco BRL
E. coli
INV
v
F
c
F
c
end
A1
rec
A1
hsd
R17 (r
k
-, m
k
+)
sup
E44
thi
-1
gyr
A96
rel
A1
80
lac
Z
'
M15
'
(
lac
ZYA-
arg
F) U169
O
-
Invitrogen
Plasmids
pBluescript II KS
Cloning and Expression vector for using with
E. coli
P
lac
Amp
r
Stratagene
pHY300PLK
Shuttle vector,
E. coli
-
B. subtilis
,
Amp
r
Tc
r
Stratagene
pCHIB1
pBluescript II KS carrying 2.58 kb
Hin
dIII fragment of chitinase gene,
chi
+
Amp
r
Thepouyporn, 1999
pHYB43
pHY300PLK carrying 2.58 kb
Hin
dIII fragment of chitinase gene,
chi
+
Amp
r
Tc
r
Lertcanawanichakul, 2000
Amp
r
ºÊ
°¥µÂ°¤¡·
·
¨¨·
(ampicillin resistance);
Tc
r
, ºÊ
°¥µÁ¦µ´
¥¨·
(Tetracycline resistance)
