full2010.pdf - page 1377

1339
0.00E+00
1.00E-06
2.00E-06
3.00E-06
4.00E-06
5.00E-06
6.00E-06
7.00E-06
8.00E-06
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45
[M]
[[M][L]]/A
y = 1.0 x10-5x + 2.0 x10 -6
(1)
[M][L]
A [L]
600 nm ‹µ„œ´Êœ‹¹ŠÄoo°¤¼¨Â°Ž°¦r¡´ œÁž„˜¦´ ¤‹µ„£µ¡š¸É 3 ´œš¹ „—´Š˜µ¦µŠš¸É 1 Á¡ºÉ°®µ‡n µ‡Šš¸É„µ¦Á„·—
µ¦ž¦³„°Á·ŠŽo°œ °Š Á‡°¦r‡¼¤·œ-ǦÁ¤¸¥¤(III) ×¥ª·›¸ ¦oµŠ„¦µ¢‹µ„¤„µ¦š¸É (1) Ž¹ÉŠÁž}œš¸É¦¼o‹´„„´ œÄœºÉ°
Benesi – Hildebrand equation
‹µ„¤„µ¦š¸É (1) ‡ªµ¤´¤¡´œ›r¦³®ªnµŠ „´ œÎµ¤µ¦oµŠ„¦µ¢Á¡ºÉ°®µ‡nµ‡Šš¸É (K) °Š„µ¦Á„·—
µ¦ž¦³„°Á·ŠŽo°œ
˜µ¦µŠš¸É 1 —Š o°¤¼ ¨‡nµ„µ¦—¼—„¨ºœÂŠ¼Š»— °Šµ¦ž¦³„°Á· ŠŽo°œÁ‡°¦r‡¼¤· œ-ǦÁ¤¸¥¤(III) š¸É‡ªµ¤¥µª‡¨ºÉœ
430 nm ¨³„µ¦‡Îµœª– Á¡ºÉ°œÎµÅž¡¨È °˜„¦µ¢®µ‡nµ‡Šš¸É „µ¦Á„·—µ¦ž¦³„°Á·ŠŽo°œ
[M] ‡º ° ‡ªµ¤Á o¤ oœ °ŠÃ¨®³, [L] ‡º° ‡ªµ¤Á o¤ oœ °Š¨·Â„œ—r , A ‡º ° ‡nµ„µ¦—¼—„¨ºœ¼Š»—š¸É‡ªµ¤¥µª‡¨ºÉ œ
£µ¡š¸É 4 —Š„µ¦¦oµŠ„¦µ¢¦³®ªnµŠ [[M][L]]/A „´ [M]
‹µ„˜µ¦µŠš¸É 1 œÎ µ¤µ¦oµŠ„¦µ¢Å—o—´Š£µ¡š¸É 4 ¡ªnµ„¦µ¢š¸Éŗo Áž}œ„¦µ¢Áoœ˜¦Š—´Š¤„µ¦š¸É (2) ‹¹Š®µ
‡nµ‡Šš¸É (K) „µ¦Á„·—µ¦ž¦³„°Á·ŠŽo°œÅ—o ‹µ„ ‡ªµ¤´œ / ‹»—˜´ —
y = 1.0 x 10-5x + 2.0 x10-6 (2)
‹µ„¤„µ¦š¸É (2) œÎµÅž‡Îµœª–®µ‡nµ‡Šš¸É„µ¦Á„·—µ¦ž¦³„°Á·ŠŽo°œ (K) ¤¸‡nµÁšnµ„´ 5.0
¨³ log K Ášnµ„´ 0.69
‡ªµ¤Á o¤ oœ °ŠÃ¨®³
ǦÁ¤¸ ¥¤(III)Å°°°œ
[M]
‡ªµ¤Á o ¤ oœ °ŠÁ‡°¦r
‡¼¤·œš¸ÉšÎ µž’·„·¦·¥µ
[L]
‡nµ„µ¦—¼—„¨ºœ¼Š»— °Š
µ¦ž¦³„°Á· ŠŽo°œš¸É‡ªµ¤
¥µª‡¨ºÉœ 430 nm
[A]
([M]x[L])/A
0.04 M 1.35 x 10-5 M 0.248 2.175 x 10-6
0.06 M 1.44 x 10-5 M 0.290 2.974 x 10-6
0.2 M 1.30 x 10-5 M 0.627 7.014 x 10-6