full2012.pdf - page 175

The economically most important plants are rice, rubber palm oil and coconut, generally produced in
monoculture plantation sometime gathered with cattle farming also the local people could spend the rest of the day in
handicraft productions.
Data collection
For data collection of economic bamboos, 100 traditional healers (65 females and 35 males) aged between 32
and 75 years, and 10-28 years skillful experience were surveyed. Direct interviews were interviewed between June to
August 2011 using a semi-structured questionnaire. Questions asked about their local knowledge or wisdom used of the
bamboos.
After interviews, the identification of economic bamboos in the fields was done. Afterwards, herbarium
specimens were prepared and photographs were taken. Plant identities were confirmed by comparison with available
voucher specimens in the Botanical Herbarium of Biology Department, Thaksin University using taxonomic keys
(Fig. 2).
Analytical Photochemistry
Extraction
Whole leaves and shoots of the identified economic bamboos were squeezed to prepare. Samples were dried in
hot-air oven at 50°C for 72 h and powdered in a blender for 1 min at maximal speed then divided into smaller aliquots
and frozen at -20°C until use. Samples (100 g) were extracted into hexane, dichloromethane, ethanol and at 25°C, 120 h.
The extracts were evaporated, lyophilized and stored at -80°C for further investigation.
Fig. 1
Map of Phatthalung province, the study site.
(modified from Phatthalung, www, 2011)
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