707
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0SFPDISPNJTǰOJMPUJDVT
)
Effects of Dietary Supplementation of Chitosan and Levamisole on the Growth
Performance, Non-specific Immune Responses and Disease Resistance of Nile tilapia
(
0SFPDISPNJTǰOJMPUJDVT
)
îóéú ýč
ÖøąÖćâÝîŤ
1*
Öùþèą đøČ
ĂÜÙúš
ć÷
2
ĒúąǰóĆ
îíÿĉ
ìíĉĝ
ēßÙÿüĆ
ÿéĉ
Öø
2
Noppadon Sukrakanchana
1*
, Kritsana Ruangkhlaii
2
and Puntasit Choksawasdikorn
2
ïìÙĆ
é÷Š
Ă
Üćîüĉ
ÝĆ
÷îĊĚ
đðŨ
îÖćøýċ
Öþćñú×ĂÜÖćøđÿøĉ
öĕÙēêĒàîǰ(chitosan) Ēúąđúüćöĉ
ēàú (levamisole) ĔîĂćĀćøêŠ
Ă
ÖćøđÝøĉ
âđêĉ
ïēêǰõĎ
öĉ
Ùčš
öÖĆ
îĒïïĕöŠ
ÝĞ
ćđóćąǰĒúąÙüćöêš
ćîìćîēøÙ×ĂÜðúćîĉ
úǰ(
Oreochromis niloticus
) ìĊę
đúĊĚ
÷ÜĔîÖøąßĆ
Üǰ
ēé÷ĔĀš
ðúćìéúĂÜĕéš
øĆ
ïĂćĀćøñÿöǰchitosan Ēúąǰlevamisole ìĊę
øąéĆ
ïêŠ
ćÜė ñúÖćøýċ
ÖþćóïüŠ
ćÖćøĔĀš
ĂćĀćøñÿö
chitosan ìĊę
øąéĆ
ïǰ1% Ēúąǰlevamisole ìĊę
øąéĆ
ïǰ250 mg/kg ĂćĀćøǰêŠ
ĂđîČę
ĂÜÖĆ
îđðŨ
îđüúćǰ12 ÿĆ
ðéćĀŤ
ǰöĊ
ñúĔîÖćø
Öøąêčš
îÖćøđÝøĉ
âđêĉ
ïēêǰĒúąúéĂĆ
êøćÖćøĒúÖđîČ
Ě
Ă×ĂÜðúćîĉ
úǰchitosan Ēúąǰlevamisole ÷Ć
ÜÖøąêčš
îõĎ
öĉ
Ùčš
öÖĆ
îĒïïĕöŠ
ÝĞ
ćđóćąǰēé÷đÞóćąǰlysozyme activityǰĒúą natural haemolytic complement activityǰ×ĂÜðúćîĉ
úĂ÷Š
ćÜöĊ
îĆ
÷ÿĞ
ćÙĆ
âǰàċę
ÜÿĆ
öóĆ
îíŤ
ÖĆ
ïĂĆ
êøćÖćøøĂéìĊę
ÿĎ
Ü×ċĚ
îđöČę
ĂđðøĊ
÷ïđìĊ
÷ïÖĆ
ïßč
éÙüïÙč
öǰĀúĆ
ÜßĆ
ÖîĞ
ćĔĀš
öĊ
Öćøêĉ
éđßČĚ
Ă
Streptococcus
agalactiae
ìĆĚ
Üǰchitosan ĒúąǰlevamisoleǰÝċ
ÜđðŨ
îÿćøìĊę
öĊ
ðøąÿĉ
ìíĉ
õćóìĆĚ
ÜĔîéš
ćîÖćøÖøąêčš
îÖćøđÝøĉ
âđêĉ
ïēêǰĒúą
õĎ
öĉ
Ùčš
öÖĆ
îĒïïĕöŠ
ÝĞ
ćđóćąǰàċę
ÜîĞ
ćĕðÿĎŠ
Öćøđóĉę
öÙüćöêš
ćîìćîêŠ
ĂđßČĚ
Ăǰ
Streptococcus agalactiae
Ĕîðúćîĉ
ú
ÙĞ
ćÿĞ
ćÙĆ
â:
ĕÙēêĒàî đúüćöĉ
ēàú õĎ
öĉ
Ùčš
öÖĆ
îĒïïĕöŠ
ÝĞ
ćđóćą Ùüćöêš
ćîìćîēøÙ ðúćîĉ
ú
Abstract
This experiment was carried out to study the effects of dietary supplementation of chitosan
and levamisole on the growth performance, non-specific immune responses and disease resistance of
Nile tilapia in cage culture. Fish were divided into 4 groups before being fed for 12 weeks with
unsupplemented commercial diet as the control, 1% of chitosan, 250 mg/kg feed of levamisole and
1% chitosan plus 250 mg/kg feed of levamisole. Results showed dietary supplementation of chitosan
and levamisole enhanced the growth with better feed conversion ratio of all treated fish significantly.
Lysozyme and complement activity were significantly enhanced in fish fed with 1% of chitosan, 250
mg/kg levamisole and 1% chitosan plus 250 mg/kg diet. The cumulative mortality was low in fish fed
with chitosan or levamisole enriched diets than those of control. These results suggest that
administration of chitosan or levamisole in the diet can be used as growth promoter, to enhance non-
specific immune responses and improve resistance of Nile tilapia to
Streptococcus agalactiae
.
Keywords:
Chitosan, Levamisole, Non-specific immunity, Disease resistance, Nile tilapia
1
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Ü 93210
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Ü 93210
*
Corresponding author: Tel. 074 443 946, E-mail address:
