เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 51

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1. Á„ȝ˜´ª°¥nµŠ¡ºžiµµ¥Á¨œ
Á„ȝ˜´ª°¥nµŠ¡º¦·Áª–‡–³ª·š¥µ«µ˜¦r¨³Áš‡ÃœÃ¨¥¸„µ¦ž¦³¤Š ‹´Š®ª´—˜¦´Š ×¥Á¨º°„œ·—¡ºš¸É¤¸
ž¦·¤µ–¤µ„Äœ¡ºÊœš¸É¨³¡ºš¸É¤¸¦µ¥Šµœ„µ¦«¹„¬µÁºÊ °Š˜oœÂ¨oªªnµ°µ‹‹³¤¸ §š›·ÍĜ„µ¦˜oµœ°œ» ¤¼¨°· ¦³ ‹Îµœªœ 8 œ·—
ŗo „n ˜oœÃ„Š„µŠÄÄ®n ˜oœÃ„Š„µŠÄÁ¨È„ ˜oœ¨Îµ¡¼ ˜oœ˜³¼ œ µª ˜oœ µ——°„ µª ˜oœ¡´Š„µ®´ª»¤—°„—Š
˜oœÃž¦ŠÂ—Š ¨³˜oœÁ˜¥š³Á¨
2. „µ¦Á˜¦¸¥¤µ¦„´ —‹µ„¡º
œÎ µ˜´ª°¥nµŠ¡º ¤µ®´ÉœÄ®oÁž}œ·ÊœÁ¨È„ Ç Ÿ¹ÉŠÄ®o®oŠ œÎ µÅž°Äœ˜¼o°š¸É °»–®£¼ ¤· 50 °Š«µÁŽ¨ÁŽ¸¥ ‹œ
®oŠœ·š ‹µ„œ´ÊœœÎµ˜´ª°¥nµŠ¤µ 100 „¦´ ¤ nĜ˜´ ªšÎµ¨³¨µ¥Á¤šµœ°¨ (methanol) š·ÊŠÅªo 7 ª´œ ×¥šÎµ„µ¦„´—ŽÊε 3
‡¦´Ê Š œÎµµ¦¨³¨µ¥š¸É „´—Å—o ˜n¨³‡¦´ÊŠ¤µ¦ª¤„´ œ ¨oª¦³Á®¥Á¤šµœ°¨°°„—oª¥Á‡¦ºÉ°Š¦³Á®¥˜´ªšÎ µ¨³¨µ¥Â®¤»œ
(rotatory evaporator) š¸É°»–®£¼¤· 55 °Š«µÁŽ¨ÁŽ¸¥ ‹œÂ®oŠ œÎµµ¦„´—®¥µÅžªµŠÄœÁ—Ž·Á‡Á˜°¦r Á¡ºÉ°—¼—‡ªµ¤ºÊœ
°°„ ‹µ„œ´ÊœœÎ µµ¦„´—š¸É Á˜¦¸¥¤Å—o ŞÄn Ĝ£µœ³š¸Éžj°Š„´ œ‡ªµ¤ºÊœÂ¨oªœÎµÅžÁ„È š¸É 4 °Š«µÁŽ¨ÁŽ¸ ¥ Á¡ºÉ°Äo
š—°®µ §š›·Í˜oµœ°œ»¤¼¨µ¦°· ¦³ ž¦·¤µ–µ¦ž¦³„°¢¨µÃªœ°¥—r ¨³ž¦·¤µ–µ¦ž¦³„°¢dœ°¨·„˜n°Åž
3. „µ¦š—°§š›·Í ˜oµœ°œ» ¤¼¨µ¦°· ¦³ °Šµ¦„´— ×¥ª· ›¸ DPPH radical scavenging assay
š—°‡ªµ¤µ¤µ¦™Äœ„µ¦—´ „‹´°œ»¤¼¨ DPPH° Ĝµ¦„´—˜µ¤ª·›¸ °Š Cotelle et al., 1996
—´—ž¨Š ˜µ¤ª·›¸ °Š Fenglin (2004) ×¥œÎµµ¦„´—š¸ÉÁ‹º °‹µŠÄœÁ¤šµœ°¨š¸ÉnªŠ‡ªµ¤Á o¤ oœš¸ÉÁ®¤µ³¤ °Šµ¦
„´—˜n¨³œ· — µ¦˜oµœ°œ»¤¼¨°· ¦³¤µ˜¦“µœ BHA (butylated hydroxyanisole) š¸É ‡ªµ¤Á o¤ oœ 0.005 – 0.5
¤·¨¨·„¦´ ¤˜n°¤·¨¨·¨·˜¦ ¨³Á¤šµœ°¨Áž}œ„µ¦š—¨°Š»—‡ª‡» ¤ ž¦·¤µ˜¦ 100 ŤÇ¦¨·˜¦ÄnĜ 96 wells microtitre
plate ˜n¨³®¨»¤Â¥„„´œ ¨oªÁ˜·¤µ¦¨³¨µ¥ 2 mM DPPH ° ĜÁ¤šµœ°¨ž¦·¤µ˜¦ 100 ŤÇ¦¨·˜¦ Ÿ¤Ä®oÁ oµ„´œÂ¨oª
n ¤š¸É°»–®£¼¤·®o °ŠÄœš¸É¤º—œµœ 30 œµš¸ œÎµÅžª´—‡n µ„µ¦—¼—„¨ºœÂŠš¸É‡nµ‡ªµ¤¥µª‡¨ºÉœ 517 œµÃœÁ¤˜¦ Áž¦¸¥Áš¸¥
„´Â¨³„µ¦š—¨°Š»— positive control Ž¹ÉŠÄo µ¦„´—š¸ÉnªŠ‡ªµ¤Á o¤ oœš¸ÉÁ®¤µ³¤ °Šµ¦„´—˜n¨³œ·—¨³Äo
Á¤šµœ°¨Âšœµ¦¨³¨µ¥°œ»¤¼ DPPH° œÎ µ‡nµš¸Éª´—Å—o¤µ‡Î µœª–®µÁž°¦rÁŽÈœ˜r„µ¦—´„‹´ °œ»¤¼¨ DPPH° ‹µ„¤„µ¦
% scavenging = [ ( Acontrol – A sample ) / Acontrol ] X 100
A sample = ‡nµ„µ¦—¼—„¨ºœÂŠ °Š»—š—°
A control = ‡nµ„µ¦—¼—„¨ºœÂŠ °Š» —‡ª‡»¤
‹µ„œ´Êœ‡Îµœª–®µ‡nµ IC50 (‡nµ‡ªµ¤Á o¤ oœ °Šµ¦„´—š¸É µ¤µ¦™—´„‹´°œ»¤¼¨ DPPH° ŗoš¸É 50 Áž°¦rÁŽÈœ˜r) ‹µ„„¦µ¢
‡ªµ¤´ ¤¡´œ›r¦³®ªnµŠ % scavenging „´ž¦· ¤µ–µ¦„´—