เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 52

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4. „µ¦ª· Á‡¦µ³®r®µž¦·¤µ–µ¦ž¦³„°¢dœ°¨·„ (total phenolic content)
®µž¦·¤µ–ᨷ¢eœ°¨ (polyphenols) ˜µ¤ª·›¸°Š AOAC (1990) ×¥œÎµµ¦„´—˜n¨³œ·—¤µÁ˜¦¸¥¤Áž}œ
µ¦¨³¨µ¥Ä®o¤¸‡ªµ¤Á o¤ oœ 5 ¤·¨¨·„¦´ ¤˜n°¤·¨¨·¨·˜¦ ĜÁ¤šµœ°¨Â¨oªÁ‹º °‹µŠÄœœÊ儨´Éœ ‹µ„œ´ÊœÁ˜· ¤µ¦¨³¨µ¥š¸ÉÁ‹º °
‹µŠž¦·¤µ˜¦ 40 ŤÇ¦¨·˜¦ ĜœÊ儨´Éœž¦·¤µ˜¦ 5 ¤·¨¨·¨·˜¦ ¨oªŸ¤„´ µ¦¨³¨µ¥ Folin –Ciocalteu’s phenol
reagent ž¦· ¤µ˜¦ 0.5 ¤·¨¨·¨·˜¦ š·ÊŠÅªoš¸É °»–®£¼¤·®o°Šœµœ 3 œµš¸ Á˜·¤ 35 % Na2CO3 Ÿ¤Ä®oÁ o µ„´œn¤š¸É°»–®£¼¤·®o°Š
œµœ 20 œµš¸ ‹µ„œ´ÊœœÎµ¤µª´—„µ¦—¼—„¨ºœÂŠš¸É‡nµ‡ªµ¤¥µª‡¨ºÉœ 750 œµÃœÁ¤˜¦ Áš¸¥„´ blank Ž¹ÉŠÄo Á¤šµœ°¨
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acid
5. „µ¦ª· Á‡¦µ³®r®µž¦·¤µ–µ¦ž¦³„°¢¨µÃªœ°¥—r
®µž¦·¤µ–¢¨µÃªœ°¥—r ×¥ž¦³¥» „˜r ˜µ¤ª·›¸ °Š Jia et al., 1999 ×¥œÎµµ¦„´—˜n¨³œ·—¤µ¨³¨µ¥
ĜÁ¤šµœ°¨ —¼—µ¦¨³¨µ¥¤µ 2.5 ¤·¨¨·¨·˜¦ ÄnĜ ª—ž¦´ ž¦·¤µ˜¦ ¨oªÁ˜·¤œÊ儨´Éœ¨ŠÅž 10 ¤·¨¨·¨·˜¦ ‹µ„œ´Êœ‹¹Š
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¤·¨¨·¨·˜¦ Á ¥nµÄ®oÁ oµ„´œÂ¨³˜´ÊŠš·ÊŠÅªo 6 œµš¸ œÎµÅžÁ˜· ¤ 1 M NaOH ž¦·¤µ˜¦ 5 ¤·¨¨·¨·˜¦ ¨oªž¦´ž¦·¤µ˜¦—oª¥œÊε
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radical scavenging assay ¨oª®µ‡nµ‡ªµ¤Á o¤ oœš¸É µ¤µ¦™¥´¥´ÊŠ°œ»¤¼¨°· ¦³Å—o 50 Áž°¦rÁŽÈœ˜r (IC50) Ÿ¨„µ¦š—¨°Š
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