เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 900

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„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª· š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
„µ¦®µ‡ªµ¤šœ˜n°°»–®£¼¤·Äœ„µ¦šÎµŠµœ °ŠÁ°œÅŽ¤rŨÁž
šÎ µ„µ¦«¹„¬µ‡ªµ¤šœ°»–®£¼¤·Äœn ªŠ°»–®£¼¤·˜´ÊŠÂ˜n 50 – 80 oC š¸ÉÁª¨µ 60 œµš¸ ×¥«¹„¬µÁ°œÅŽ¤rŨÁžš´Ê
ŠÄœµ¦„´—®¥µ ‹µ„„µ¦Â¥„—o ª¥¦³ ATPS š´ÊŠ´ÊœÁ„¨º°Â¨³ Polyethylene glycol —´Š˜µ¦µŠš¸É 5
˜µ¦µŠš¸É 5 —Š‡ªµ¤šœ˜n°°»–®£¼¤·°ŠÁ°œÅŽ¤rŨÁžš¸É°»–£¼¤·˜nµŠ Ç Áž}œÁª¨µ 1 ´ÉªÃ¤Š
°»–®£¼¤·
(oC)
Crude activity
(Units/ml) % activity PEG phase activity
(Units/ml) % activity Salt phase activity
(Units/ml) % activity
Control 0.254± 0.001 100.00 0.161± 0.007 100.00 0.279 ± 0.001 100.00
50 0.247 ± 0.001 96.86 0.148 ± 0.007 91.86 0.262 ± 0.001 93.94
60 0.254 ± 0.011 99.85 0.180 ± 0.008 112.02 0.305 ± 0.003 109.43
70 0.226 ± 0.006 88.63 0.139 ± 0.002 86.72 0.215 ± 0.001 77.08
80 0.199 ± 0.006 78.40 0.137 ± 0.002 85.14 0.187 ± 0.003 67.06
‹µ„˜µ¦µŠš¸É 5 ‹³Á®ÈœÅ—oªnµ‡ªµ¤šœ°»–®£¼¤·°ŠÅ¨ÁžÄœ´Êœ PEG ‹³µ¤µ¦™šœ°»–®£¼¤·Å—o—¸ÁœºÉ°Š‹µ„¤¸
% relative activity š¸É°»–®£¼¤· 80 oC Á®¨º°°¥¼n ™¹Š 85.14 % Ĝ –³š¸É´ÊœÁ„¨º°Á°œÅŽ¤r‹³‡ŠÁ®¨º° 67.06%ÁœºÉ°Š‹µ„
PEG Áž} œ¡°¨·Á¤°¦rš¸É¤¸œµ—Ä®n¨³¤¸ hydroxyl group ‹Îµœªœ¤µ„šÎµÄ®o nªœš¸É¤¸´Êª °ŠÁ°œÅŽ¤rŨÁžµ¤µ¦™‹´
„´œ—oª¥¡´œ›³Å±Ã—¦Á‹œ„´„¦—°³¤·ÃœÄœÃ¤Á¨„»¨ °¸„š´ÊŠÁ°œÅŽ¤rŨÁž¥´Š¤¸ nªœš¸ÉŤn¤¸´Êª°¥¼n Áž}œ‹Îµœªœ¤µ„Ž¹ÉŠ‹³
Á„·— interaction „´n ªœÅ¤n¤¸´Êª °Š PEG šÎµÄ®o¤¸„µ¦ž„žj°ŠÃ‡¦Š¦oµŠÃž¦˜¸œ °ŠÁ°œÅŽ¤rŨÁžnŠŸ¨Ä®o Á°œÅŽ¤r
¥´Šµ¤µ¦šÎµŠµœÅ—o —¸š¸É°»–®£¼¤· ¼Š —´Š‹³Á®ÈœÂœªÃœo¤„µ¦¨—¨ŠÅ—o‹µ„£µ¡š¸É 2
£µ¡š¸É 4 —Š‡ªµ¤šœ˜n°°»–®£¼¤·°ŠÁ°œÅŽ¤rŨÁžš¸É°»–£¼¤·˜nµŠ Ç Áž}œÁª¨µ 1 ´ÉªÃ¤Š
„µ¦Á¦nŠž’·„·¦·¥µš¦µœÁ°Áš°¦·¢dÁ‡´œ(transesterification)
‹µ„„µ¦«¹„¬µž’·„·¦·¥µš¦µœÁ°Áš°¦· ¢dÁ‡´œš¸É¤¸Á°œÅŽ¤rŨÁž Áž}œ˜´ ªÁ¦nŠž’·„·¦·¥µ °´ ˜¦µnªœ œÊε¤´œ ˜n °
Á¤šµœ°¨ ˜n ° Á°œÅŽ¤rŨÁž‡º° 10:2:1 ¨³°»–®£¼¤·š¸ÉÁ®¤µ³¤ ‡º ° °»–®£¼¤· 40 oC ‹³Å—oÁž°¦rÁŽÈœ˜r yield °Š
ŝ𗸠ÁŽ¨ 75% ‹µ„„µ¦«¹„¬µÁž¦¸¥Áš¸¥‡»–¤´˜·°ŠÅÃ°—¸ÁŽ¨š¸ÉÁ¦nŠ—oª¥ÁÂ¨³Á¦n Š—oª¥Á°œÅŽ¤rŨÁž„µ¦
ª·Á‡¦µ³®r —oª¥Áš‡œ·‡ TLC Ž¹ÉŠÄo˜´ªšÎµ¨³¨µ¥Ÿ¤¦³®ªnµŠ Hexane : Acetic acid : Diethly ether Ĝ°´ ˜¦µnªœ 2:7:3