เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 894

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„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª· š¥µ¨´¥š´„¬·– ‡¦´ÊŠš¸É 19 ž¦³‹Îµže 2552
protein were 44.38, 19.72 and 9.64 kDa. The salt phase was separated by Sephadex G 100 column and the
chromatogram showed 5 major protein peaks, second peak had lipase activity (0.121±0.007 Units/ml), while PEG
phase showed 8 major protein peaks, seventh peak had lipase activity (0.098±0.003 Units/ml). Two lipase activity
peaks were assumed to be 2 isozyme conformation in cocoanut had been purified by ATPS. The PEG phase lipase
was found to be stable in temperature range 50-80 oC and at 80 oC for 1 h the activity was 85.14 % but the salt
phase remain 67.04 %. The resultant from transesterification reaction by using PEG lipase(15.34 units/ml) as
biocatalytic synthesis of methyl esters at 40 oC, maximum methyl esters yield was 75 % and this obtained
biodiesel had similarly physical and chemical property with biodiesel of NaOH catalyzing, to confirm obtained
biodiesel was used TLC, found that Rf value of lipase biodiesel and base biodiesel to be approximately 0.71 and
0.78, the relative viscosity comparing with water was found 1.6 and 2.6 respectively.
Keywords : Lipase; Aqueous two-phase systems, ATPS; Transesterification; biodiesel ; relative viscosity
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