เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 927

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„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´„¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
Ş°¥¼nĜ´Êœ PEG ¤¸ %Yield Ášnµ„´ 90.26% nªœÁ°œÅŽ¤r ÁŽ¨¨¼Á¨‹³Â¥„Åž°¥¼n Ĝ´ÊœÁ„¨º ° ¤¸ %Yield Ášnµ„´
78.86% ¨³¤¸ ‡nµ Kprotease ¨³‡nµ Kcellulase Ášnµ„´ 6.50 ¨³ 0.48 ˜µ¤¨Î µ—´
£µ¡š¸É 1 —Š„¦µ¢ Binomial curve °Š¦³µ¦¨³¨µ¥°Šª´’£µ‡Ã—¥Äo PEG 1500 š¸É‡ªµ¤Á o¤ oœ 5%, 10%,
15% ¨³ 20% w/v ¨³ÄoÁ„¨º ° 3 œ·— ŗo „n Ammonium sulphate, Dipotassium hydrogen phosphate
¨³ Tripotassium citrate
˜µ¦µŠš¸É 1 —Šž¦·¤µ–Á°œÅŽ¤r ÁŽ¨¨¼Á¨Â¨³Ãž¦˜· Á°Äœ´Êœµ¦¨³¨µ¥¡°¨·Á¤°¦r¨³µ¦¨³¨µ¥Á„¨º°
Salt %PEG %Salt
PEG phase Salt phase Kprotease Kcellulase
Protease
(Units)
% Yield
protease
Cellulase
(Units)
%Yield
cellulase
Protease
(Units)
% Yield
protease
Cellulase
(Units)
%Yield
cellulase
K-citrate 5
10
15
20
50.4
37.2
36.2
31.6
22.17
37.66
50.17
78.18
14.04
23.84
31.76
49.50
19.32
52.32
62.56
120.78
8.63
23.36
27.93
53.92
83.84
50.04
33.96
19..95
53.08
31.68
21.50
12.63
338.50
311.32
287.28
185.28
151.11
138.98
128.25
82.71
0.26
0.75
1.48
3.92
0.06
0.17
0.22
0.65
K2HPO4 5
10
15
20
21.2
20.1
16.4
13.0
19.20
42.39
59.85
65.07
12.58
26.84
37.89
41.20
34.68
71.40
121.44
136.40
15.48
31.88
54.21
60.89
34.02
32.14
25.25
21.38
21.58
20.37
15.99
13.54
267.20
269.28
204.12
160.44
119.29
120.21
91.13
71.63
0.56
1.32
2.37
3.04
0.13
0.27
0.59
0.85
(NH4)2SO 4 5
10
15
20
28.6
26.2
19.2
16.0
47.66
87.78
142.56
145.34
30.17
55.58
90.26
92.02
21.02
50.60
85.28
93.60
9.39
22.59
38.59
41.79
40.21
30.76
21.95
19.33
25.46
19.47
13.89
12.24
214.20
214.40
176.64
174.84
95.62
95.71
78.86
78.05
1.19
2.85
6.50
7.52
0.16
0.24
0.48
0.54
œÊ室´„Áž¨º°„´ž³¦— 157.94 100 224.00 100.00 157.94 100 224.00 100.00