เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 925

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„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´„¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
ATPS contained 15% PEG and 19.2% Ammonium sulphate salts was the best ratio for cellulose and protease
isolation. Protease was isolated into PEG phase 90.26% and cellulose was isolated into salt phase 78.86%. Kprotease
and K cellulase were 6.50 and 0.48, respectively. Purified protease and cellulose after partition by using Sepharose 6B
30 cm × 2 cm column, 50 mM Tris-HCl buffer pH 7.0 and flow rate 0.22 ml/min showed that protease had 3.83
purification fold and 48.46 %Yield while cellulase had 2.71 purification fold and 64.04 %Yield. Determined
molecular weight of protease found that there was 2 sub units consist of 20.0 and 19.3 kDa and cellulose had 1 sub
unit contained 7.3 kDa.
Keywords : Cellulase; Protease; Pine apple rinds fermented juice; Aqueous two-phase system
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