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%PEG
(w/v)
% Trisodium citrate (w/v)
PEG1500 PEG 6000
5 50 26
10 30 18
15 20 15
20 17 13
25 15 12
30 13 11
35 12 10
40 11 9
£µ¡š¸É 2 Á¢Å—°³Â„¦¤„µ¦Â¥„¦³®ªnµŠ PEG 1500(a) ¨³ PEG 6000(b) „´Á„¨º° trisodium citrate
‹µ„„µ¦š—¨°Šš¸Éŗo —ŠÄ®oÁ®Èœªnµ PEG š¸É¤¸œµ—äÁ¨„»¨Ä®n ‹³Äož¦· ¤µ–Á„¨º°Äœ„µ¦Â¥„´Êœœo°¥„ªn µ PEG
äÁ¨„» ¨ œµ—Á¨È„Á¤ºÉ °Äo Ĝž¦·¤µ–š¸ÉÁšnµ„´œ ×¥š´ÉªÅž¡ªn µ™oµÄo¡°¨· Á¤°¦rš¸É¤¸œÊÎ µ®œ´„äÁ¨„» ¨œo°¥‹³šÎ µÄ®o¤¸
ž¦· ¤µ–´Êœ¨nµŠ (bottom phase) Á¡·É¤¤µ„ ¹Ê œ Á¤ºÉ°Áš¸ ¥‡ªµ¤Á¦ÈªÄœ„µ¦Â¥„´Êœ˜µ¤ œµ— °Š¡°¨·Á¤°¦r ¡ªnµ™oµÄo
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Áž°¦r ÁŽœ˜r¡°¨·Á¤°¦rÁšnµ„´œ —´Šœ´Êœ™o µ®µ„˜o°Š„µ¦Ä®oŗoÁ°œÅŽ¤rš¸É ¤¸‡ªµ¤Á o¤ oœ¼ŠÁ¡ºÉ °Ä®o°¥¼nĜ´Êœ PEG —oµœœ
‹³˜o°ŠÄo¡°¨·Á¤°¦r œµ—Á¨È„¨³Äo Ĝž¦·¤µ–œo°¥—´Š‹³´ŠÁ„˜¸°Š´Ê œ PEG š¸ÉčoÁž°¦rÁŽÈ œ˜r˜Éε Ç ‹³¤¸ ¸Á o¤
Ÿ¨„µ¦«¹„¬µ„µ¦Â¥„Á°œÅŽ¤rŨÁžÄœµ¦„´ —®¥µ—oª¥¦³ ATPS ץčo¡°¨·Á¤°¦r PEG œµ— 1500 ¨³
6000 „´Á„¨º° trisodium citrate
„µ¦«¹„¬µ„µ¦Â¥„Á°œÅŽ¤rŨÁžÄœµ¦„´ —®¥µÁ o¤ oœ—oª¥¦³ ATPS ץčo PEG „´Á„¨º°
trisodium citrate ŗoŸ¨„µ¦š—¨°Š—´Š£µ¡š¸É 3(a) ¨³ 3(b) ˜´ÊŠš·ÊŠÅªo Ä®oÂ¥„´Êœ°¥nµŠ¤¼¦–r ¨oªÂ¥„´Êœ PEG
—oµœœÂ¨³µ¦¨³¨µ¥Á„¨º°´Êœ¨nµŠ œÎµ¤µ«¹„¬µž¦·¤µ–Þ¦˜¸œ ¨³ž¦·¤µ–Á°œÅŽ¤rŨÁž Á¡ºÉ°®µ‡ªµ¤¦· »š›·Í°Š
Á°œÅŽ¤r(purification factor) %yield ¨³‡nµ partition coefficient ( K) —´Š˜µ¦µŠš¸É 3 ¨³µ¤µ¦™‡Îµœª–Å—o—´Šœ¸Ê
(a) (b)