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×¥šÎµ„µ¦n¤Á°œÅŽ¤rŪoš¸É°»–®£¼¤·˜nµŠ Ç Áž}œÁª¨µ 1 ¤. ¨o ªœÎµÅžÁŽœ˜¦·¢d ª„rš¸É 9170 ×g Á¡ºÉ°Â¥„˜³„°œ‹µ„œ´Êœ
œÎ µÅž®µ‡nµÂ°‡˜·ª·˜¸ÊÁ°œÅŽ¤rŨÁž—´Š o°¤¼¨Äœ˜µ¦µŠš¸É 5 µ¤µ¦™œÎµ¤µÁ ¸¥œ„¦µ¢Â—Š‡ªµ¤´¤¡´œ›r¦³®ªnµŠ‡nµ
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®¥µÂ¨³Äœ´Êœ PEG ‹³µ¤µ¦™š¸É‹³šÎµŠµœÅ—oĜn ªŠ°»–®£¼¤·š¸É „ªoµŠ‡º° 40 – 80 oC ×¥š¸É CLP1500 ¨³CLP6000
š¸É 70 oC ¥´Š‡Š¤¸‡nµÂ°‡˜·ª·˜¸Ê Áž}œ 106.26 ¨³ 136.69% ˜µ¤¨Îµ—´ Ž¹ÉŠÁ°œÅŽ¤rĜ CLP6000 ‹³µ¤µ¦™šœ°»–®£¼¤·
ŗo —¸ ÁœºÉ°Š‹µ„ PEG 6000 äÁ¨„»¨š¸É¤¸œµ—Ä®n„ªn µ PEG 1500 ¨³¤¸®¤¼nű—¦°„Ž·¨š¸É µ¤µ¦™Á„· —¡´œ›³Å±Ã—¦Á‹œ
„´„¦—°³¤·Ãœš¸ÉÁž}œÃ¤Á¨„»¨ °ŠÁ°œÅŽ¤r °¸„š´ÊŠÁ°œÅŽ¤rŨÁž¥´Š¤¸ ‡ªµ¤Áž}œ hydrophobic ‹¹ Šµ¤µ¦™Á„·—¡´œ›³
—oª¥¡´œ›³Ã‡ªµÁ¨œšr „´ PEG ŗo °¸„—oª¥ (Vermue and Tramper, 1995) šÎ µÄ®oǦŠ¦oµŠ¤¸ „µ¦Áž¨¸É¥œÂž¨Šœo°¥ ‹¹Š
nŠŸ¨Ä®o Á¤ºÉ°Å—o¦´‡ªµ¤¦o °œ‹³¤¸„µ¦Á¸¥£µ¡Å—o¥µ„„ªnµÁ°œÅŽ¤r°· ¦³
˜µ¦µŠš¸É 5 —Š‡ªµ¤šœ˜n°°»–®£¼¤·°ŠÁ°œÅŽ¤rŨÁžš¸Éŗo ‹µ„„µ¦Â¥„—oª¥ ATPS
Temp.
(o C)
Crude
(Units/ml)
%
Activity
PEG 1500
(Units/ml)
%
Activity
Salt 1500
(Units/ml)
%
Activity
PEG 6000
(Units/ml)
%
Activity
Salt 6000
(Units/ml)
%
Activity
Control 0.025 100.00 0.042 100.00 0.009 100.00 0.025 100.00 0.017 100.00
40 0.031 122.19 0.044 105.48 0.011 119.63 0.029 111.69 0.021 122.90
50 0.026 105.80 0.047 111.42 0.012 133.23 0.031 117.85 0.019 112.09
60 0.023 93.74 0.051 121.85 0.011 119.11 0.034 128.90 0.018 105.44
70 0.022 89.60 0.045 106.26 0.009 99.22 0.036 136.69 0.019 114.03
80 0.017 69.25 0.036 85.52 0.008 87.71 0.027 102.46 0.013 74.41
90 0.011 42.88 0.026 61.86 0.006 64.68 0.017 67.75 0.009 53.08