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‹µ„˜µ¦µŠš¸É 6 ‹³Á®ÈœÅ—oªnµÁ°œÅŽ¤rŨÁžÄœ PEG 1500 ‹³¤¸‡nµÂ°‡˜·ª·˜¸Êš¸É ˜„˜nµŠ„´œÅž˜µ¤µ¦š¸ÉœÎµ¤µ
«¹„¬µŸ¨š¸É¤¸˜n°‡nµÂ°‡˜·ª·˜¸Ê ×¥Ÿ¨ °ŠÂ‡šÅ°°°œ (Cations) Ánœ Ag+ Ca2+ Mg2+ Co 2+ ¨³ Cu2+ ‹³¤¸Ÿ¨Äœ„µ¦
¥´¥´ÊŠ‡nµÂ°‡˜· ª·˜¸ÊÁ¤ºÉ °ŸnµœÅž 1 ¤ ‹³¤¸Ÿ¨Äœ„µ¦¨—‡nµÂ°‡˜· ª·˜¸Êš´Ê Šš¸É‡ªµ¤Á o ¤ oœ 1 ¨³ 10 mM ×¥š¸ÉÅ°°°œ Co2+
¨³Cu2+ ‹³µ¤µ¦™¥´ ¥´ÊŠ‡nµÂ°‡˜·ª·˜¸Êŗo ¼Š»—š¸É‡ªµ¤Á o¤ oœ 10 mM ¤¸‡ªµ¤µ¤µ¦™Äœ„µ¦¥´¥´ÊŠ™¹Š 53.37 ¨³
35.58 % ˜µ¤¨Î µ—´ Ž¹ÉŠ°—‡¨o°Š„´Ÿ¨Äœ„µ¦¥´¥´ÊŠÅ¨Ážš¸É¤µ‹µ„ÁºÊ°‹»¨·œš¦¸¥r (Côté and Shareck, 2008) nªœ
µ¦ž¦³„°š¸ÉÁž}œ˜´ª¥´¥´ÊŠ„µ¦šÎ µŠµœ °ŠÁ°œÅŽ¤rŗo„n PMSF EDTA ¨³ DTT ‹³¤¸Ÿ¨Äœ„µ¦n ŠÁ¦·¤Ä®o¤¸‡nµÂ°
‡˜·ª·˜¸ÊÁ¡·É¤ ¹Êœ Ž¹ÉŠ EDTA ¨³ DTT µ¤µ¦™Á¡·É¤‡nµÂ°‡˜·ª·˜¸Êŗo 120.19 ¨³ 264.42 % ˜µ¤¨Î µ—´
¦»žŸ¨„µ¦ª·‹´¥
„µ¦Â¥„Á°œÅŽ¤r ŨÁž‹µ„nªœš¸ÉÁ®¨º°š·ÊŠ °Š„¦³ªœ„µ¦„´ —œÊε¤´œ¤³¡¦oµª ץčo aqueous two-
phase systems (ATPS) Áž} œª·›¸ š¸ÉŠnµ¥ µ¤µ¦™„´ —Â¥„Á°œÅŽ¤rŨÁžÅ—ož¦·¤µ–Á°œÅŽ¤r 0.0718 Units/mg ‡nµ
Partition coefficient ( Klipase) Ášnµ„´ 5.03 ¨³¤¸‡ªµ¤¦· »š›·ÍÁ¡·É¤ ¹Êœ 231 Ášnµ Á¤ºÉ°Áš¸ ¥„´µ¦„´ —®¥µ µ¤µ¦™
šÎ µŠµœÅ—o—¸Äœ°»–®£¼¤·ÄœnªŠ„ªoµŠ °¸„š´ÊŠ¥´ Š¤¸o°—¸ÁœºÉ°Š‹µ„ŨÁž‹³™¼„„´ —Ä®o°¥¼n Ĝ´Êœ PEG šÎµÄ®o¤¸ nªœn ª¥Äœ
„µ¦„µ¦žj°Š„´œÁ°œÅŽ¤r Ä®o µ¤µ¦™šœ˜n°°»–®£¼ ¤· ¼ŠÅ—o—¸ ¨³¥´ ŠÁž}œ„µ¦Á¡·É ¤¤¼¨‡nµ °Šš¸É Á®¨º°š·ÊŠÄ®o µ¤µ¦™œÎµ
„¨´¤µÄoÄ®oÁ„·—ž¦³Ã¥œr
‡Îµ °‡»–
° °¡¦³‡» –´–”·˜ª·š¥µ¨´¥ ¨³µ µª·µÁ‡¤¸ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´¥š´„¬·– š¸ÉŗoÄ®o„µ¦
œ´ œ»œÄœ—oµœÁŠ· œš»œ ‡¦»£´–”rÁ¡ºÉ°šÎµ„µ¦ª·‹´¥ ¨³„µ¦œÎµÁœ°
Á°„µ¦°oµŠ°·Š
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