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‹µ„Ÿ¨„µ¦š—¨°Š„µ¦Äo 5% PEG ‹³Å¤n µ¤µ¦™Å—o´Êœ PEG ÁœºÉ °Š‹µ„¤¸ž¦·¤µ– PEG œo °¥Á„·œÅž —´Š o°¤¼¨‹µ„
˜µ¦µŠš¸É 3 ¡ªnµÁ¤ºÉ°Äo % PEG ¨³ % trisodium citrate ˜µ¤ phase diagram ‹µ„Ÿ¨„µ¦š—¨°Šš¸ÉŸnµœ¤µ‹³µ¤µ¦™
šÎ µ„µ¦Â¥„Á°œÅŽ¤rŨÁž ×¥š¸É 10 % PEG ¡¤¸Á°œÅŽ¤rŨÁžÄœ´Êœ PEG °¥¼n ¼Š™¹Š 0.072 Units/mg protein ‡nµ
Partition coeffient(Klipase) Ášnµ„´ 5.03 ¨³¤¸‡ªµ¤¦· »š›·ÍÁ¡·É¤ ¹Êœ 231 Ášnµ ‹¹ ŠÁ¨º°„Ä®oÁž}œ£µª³š¸ÉÁ®¤µ³¤Äœ„µ¦
œÎ µÅžÄo˜n°Åž ™¹ŠÂ¤oªnµ % yield š¸Éŗo ‹³œo°¥„ªnµ„µ¦Äo PEG š¸ÉÁž°¦rÁŽÈœ°ºÉœ Ç ÁœºÉ°Š‹µ„¤¸ ‡ªµ¤¦· »š›·Íš¸É ¼Š Á®¤µ³
„n„µ¦œÎµÅžÄo ˜nÁ¤ºÉ°Á¡·É¤Áž°¦rÁŽÈ œ˜r°Š PEG ‹³šÎµÄ®oÁ°œÅŽ¤r¤¸ÂœªÃœo¤š¸É ‹³°¥¼n´Êœœ¨—œo°¥¨Š °´œÁœºÉ°Š¤µ‹µ„
‹³Äo ž¦·¤µ–Á„¨º°¨—¨ŠšÎµÄ®o‡ªµ¤¤¸´Êª„È‹³œo°¥¨Š Þ¦˜¸œÃ—¥n ªœ¤µ„‹³¥´Š‡Š°¥¼nĜ´Êœ¨n µŠ (´ÊœÁ„¨º °) Á¤ºÉ °
Áž¦¸ ¥Áš¸¥„´µ¦„´—®¥µ ‹³¤¸ž¦·¤µ–Á°œÅŽ¤r œo°¥„ªnµ¤µ„ ¨³¥´Š¡ªnµ œµ— °Š¡°¨·Á¤°¦r‹³¤¸Ÿ¨Äœ„µ¦Â¥„
Ä®oÁ°œÅŽ¤r ÁœºÉ°Š‹µ„„µ¦Á¡·É¤ œµ— °ŠÃ¤Á¨„»¨‹³nŠŸ¨Ä®o¤¸¡ºÊœš¸ÉªnµŠÄœ´Ê œœœo°¥¨Š‹¹ŠšÎµÄ®o‡nµ Klipase ¨—¨Š
(Naganagouda and Mulimani, 2008)(—´Š˜µ¦µŠš¸É3) ¨³Á°œÅŽ¤rŨÁž‹³¤¸ ž¦·¤µ–¤µ„Äœ´Êœ PEG Ž¹É Š¤¸‡»–¤´˜·
Áž}œ hydrophobic lipase
˜µ¦µŠš¸É 3 —Šž¦·¤µ–Á°œÅŽ¤rŨÁž‡ªµ¤¦· »š›·Í°ŠÁ°œÅŽ¤r Partition coefficient (Klipase) ¨³ %Yield ץčo
PEG 1500 ¨³Á„¨º° trisodium citrate
PEG
Mol.Wt.
PEG
(w/v%)
TriNa-citrate
(w/v%)
Top phase
Activity(U/ml)
Bottom phase
Activity(U/ml)
Total
volume(ml)
Klipase Top phase(PEG phase)
SP PF %Yield
1500
5 50 - 0.004 10.0 - - - -
10 30 0.050 0.010 10.5 5.03 0.072 231.68 30.81
15 20 0.033 0.015 10.5 2.25 0.051 165.22 34.04
20 17 0.023 0.014 11.5 1.68 0.044 140.38 43.23
25 15 0.021 0.017 12.0 1.26 0.037 117.89 51.70
30 13 0.016 0.019 12.5 0.82 0.031 98.99 51.73
35 12 0.015 0.019 12.0 0.80 0.031 100.20 65.10
40 11 0.011 0.022 12.0 0.51 0.021 67.30 43.75