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งที่
20 ประจำป
2553
- 8 -
Evaluation of methods for preparing hydrogen-producing seed inocula under
thermophilic condition by process performance and microbial community
analysis
Sompong O-Thong
a
, Poonsuk Prasertsan
b,*
and Nils-Kåre Birkeland
c
a
Department of Biology, Faculty of Science, Thaksin University, Patthalung 93110, Thailand
b
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University,
Songkhla 90112, Thailand
c
Department of Biology and Centre for Geobiology, University of Bergen, P.O. Box 7800, N-5020,
Bergen, Norway
Abstract
Five methods for preparation of hydrogen-producing seeds (base, acid, 2-bromoethane-
sulfonic acid (BESA), load-shock and heat shock treatments) as well as an untreated anaerobic
digested sludge were compared for their hydrogen production performance and responsible
microbial community structures under thermophilic condition (60ºC). The results showed that
the load-shock treatment method was the best for enriching thermophilic hydrogen-producing
seeds from mixed anaerobic cultures as it completely repressed methanogenic activity and gave
the a maximum hydrogen production yield of 1.96 mol H
2
mol
-1
hexose with an hydrogen
production rate of 11.2mmol H
2
l
-1
h
-1
. Load-shock and heat-shock treatments resulted in a dominance
of
Thermoanaerobacterium thermosaccharolyticum
with acetic acid and butyric acid type of
fermentation while base- and acid-treated seeds were dominated by
Clostridium
sp. and BESA
treated seeds were dominated by
Bacillus
sp. The comparative experimental results from hydrogen
production performance and microbial community analysis showed that the load-shock treatment
method was better than the other four methods for enriching thermophilic hydrogen producing
seeds from anaerobic digested sludge. Load-shock treated sludge was implemented in palm oil mill
effluent (POME) fermentation and was found to give maximum hydrogen production rates
of 13.34 mmol H
2
l
-1
h
-1
and resulted in a dominance of
Thermoanaerobacterium
spp. Load-shock
treatment is an easy and practical method for enriching thermophilic hydrogen producing
bacteria from anaerobic digested sludge.
Keywords:
Thermophilic hydrogen-producing seed; Base-treatment; Acid-treatment; BESA-
treatment; Load-shock treatment; Heat-shock treatment; Microbial community structure
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