เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 804

„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´ „¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
1
‡» –£µ¡šµŠ‹»¨¸ªª·š¥µ °Š¢°ŠœÊε¨oµŠ‹µœ‹µ„¦oµœ°µ®µ¦¤®µª·š¥µ¨´¥š´„¬·– ª·š¥µÁ ˜Š ¨µ
Microbiological Quality of Dish Washing Sponges
from Cafeteria at Thaksin University, Songkhla Campus
» ¬„¦ °»˜¦£·µ˜· 1* ‹´œš¦r›· ¦µ —ªŠ‹´œš¦r 2 ¨³ ‹¦ª¥¡¦ „µ‹œÃ˜·3
Bussakorn Utarapichat 1 *, Jantira Duangjan2 and Jaruayporn Kanchanachote3
š‡´—¥n°
¢°ŠœÊÎ µ¨oµŠ‹µœ¤´ „¤¸Á«¬°µ®µ¦Â¨³‡ªµ¤ºÊœ˜„‡oµŠ šÎµÄ®o‹»¨·œš¦¸¥rš¸É žœÁžg°œÁ˜·Ã˜Å—o ˜n„µ¦˜¦ª‹®µ
ÁºÊ °Ã¦‡šµŠÁ—·œ°µ®µ¦¤¸ª·›¸ „µ¦¥»nŠ¥µ„„ªn µ „µ¦˜¦ª‹Ã‡¨·¢°¦r¤Â¨³ Escherichia coli Ž¹ÉŠÁž}œÂ‡š¸Á¦¸ ¥¨ÎµÅo
°Š´˜ªr Á¨º °—°»n œ ¨³Äo Áž} œ—´œ¸ n Š¸Ê„µ¦žœÁžg°œ °Š°» ‹‹µ¦³Äœ°µ®µ¦ œÊÎ µÂ¨³Á‡¦ºÉ °Š—ºÉ¤ „µ¦«¹ „¬µœ¸Ê
¤¸ª´˜™»ž¦³Š‡r Á¡ºÉ°˜¦ª‹ž¦·¤µ–‹» ¨·œš¦¸¥rš´ÊŠ®¤— ž¦· ¤µ– MPN /g °ŠÃ‡¨·¢°¦r¤ ¨³ E. coli Ĝ¢°ŠœÊε¨oµŠ‹µœ
‹µ„¦oµœ°µ®µ¦Äœ¤®µª· š¥µ¨´¥š´ „¬·– ª·š¥µÁ ˜Š ¨µ ‹Îµœªœ 13 ¦oµœ ˜n ¨³¦oµœ˜¦ª‹ 3 ‡¦´Ê Š ˜n¨³‡¦´ÊŠ®nµŠ„´ œ
1 Á—º°œ ¦ª¤ 39 ˜´ª°¥n µŠ Ÿ¨„µ¦˜¦ª‹‡¦´ÊŠš¸É 1 2 ¨³ 3 ¡‹» ¨·œš¦¸¥rš´ÊŠ®¤—¤¸ž¦·¤µ– 2.3 x 107 – 1.0 x 109,
3.5 x 10 7– 5.3 x 109 ¨³ 3.6 x 107 – 3.0 x 109 CFU/g ˜µ¤¨Îµ—´ ž¦·¤µ–Ǩ·¢°¦r¤ 530 – >1,100 ; 530 – >1,100
¨³ 750 – >1,100 MPN /g ˜µ¤¨Î µ—´ ž¦·¤µ– E. coli 30 – 230 , 30 – 150 ¨³ 62 – 430 MPN /g ˜µ¤¨Îµ—´
ÁœºÉ°Š‹µ„¥´ŠÅ¤n ¤¸Á„–”r„ε®œ—‡»–£µ¡šµŠ‹» ¨¸ªª·š¥µ °Š¢°ŠœÊε¨oµŠ‹µœ ‹¹ŠÁš¸¥Ÿ¨„µ¦«¹„¬µœ¸Ê„´Á„–”r‡»–£µ¡
šµŠ‹» ¨¸ªª· š¥µ °Š°µ®µ¦ž¦»Š» „š´ÉªÅž ¨³£µœ³´ ¤Ÿ´ °µ®µ¦š¸É„ε®œ—×¥„¦¤ª·š¥µ«µ˜¦r „µ¦Â¡š¥r
„¦³š¦ªŠµ›µ¦–» ¡ªn µ¢°ŠœÊε¨o µŠ‹µœš»„˜´ ª°¥nµŠ¤¸ž¦· ¤µ–‹» ¨·œš¦¸ ¥rš» „œ· —¼ Š„ªn µÁ„–”r‡»–£µ¡
šµŠ‹»¨¸ªª·š¥µ—´Š„¨n µª Ž¹ÉŠnŠ¸ÊªnµÄœ¢°ŠœÊÎ µ¨oµŠ‹µœ°µ‹¤¸ÁºÊ°Ã¦‡šµŠÁ—·œ°µ®µ¦¼ Šš¸É°µ‹Â¡¦nÁºÊ°Åž¥´ ŠŸ¼o¦·Ã£‡Å—o
‡ÎµÎµ‡´ : ‡»–£µ¡ ‹»¨¸ªª·š¥µ ¢°ŠœÊε¨oµŠ‹µœ ¦o µœ°µ®µ¦ ¤®µª·š¥µ¨´¥š´„¬·–
Abstract
Dish washing sponges always have the residual food and moisture left; therefore, the contaminated
microorganisms can grow. The detection methods of foodborne pathogens are more difficult than the detection
methods of coliforms and Escherichia coli which are the enteric bacteria of warm-blooded animals and are used
as the indicator of fecal contamination in foods, water and beverages. The aims of this study were to enumerate
total microorganisms, MPN/g of coliforms and E. coli in 39 dish washing sponges from 13 cafeterias which were
evaluated 3 times at one month intervals at Thaksin University,Songkhla campus .The results from the first, second
1* Ÿ¼onª¥«µ˜¦µ‹µ¦¥r £µ‡ª· µ¸ªª·š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª· š¥µ¨´¥š´„¬·– ª·š¥µÁ ˜¡´ š¨»Š
2 œ· ·˜£µ‡ª· µ¸ªª·š¥µ ‡–³ª·š¥µ«µ˜¦r ¤®µª·š¥µ¨´¥š´„¬·–
3 ¡¥µµ¨ª·µ¸ ¡ ¤®µª·š¥µ¨´¥š´„¬·– ª·š¥µÁ ˜Š ¨µ
* Corresponding author: Ú¦«´¡šr/ Ú¦µ¦: 0-74693992 e-mail : bussakorn99@hotmail.com