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The quantity of caffeine from o/w emulsion on the skin did not differ from w/o emulsion. The caffeine from both
type of tested emulsion did penetrate through the skin not so well, because there were not any enhancers in the
formulation of both types of emulsion. So the safety caffeine cream to be formulated might be w/o emulsion,
which caffeine was not so much penetrated into blood stream. However the formulation must be further developed
for a better penetration into cutaneous layer of active anti-cellulite caffeine.
Table 2 Content of caffeine from both type of emulsion on the various layers of the applied skin. After 24 hr
of testing (origin concentration of caffeine was 30 mg/ml and n = 3)
Location of caffeine quantified
w/o emulsion
o/w emulsion
Caffeine on the donor side surface (mg/ml)
29.92 ± 1.25
29.86 ± 6.82
Caffeine in the skin (ôg/ml)
40.55 ± 0.36
18.79 ± 2.59
Caffeine in the receptor compartment (ôg/ml)
35.07 ± 6.05
75.75 ± 24.84
Conclusion
On the basis of these results, the caffeine absorption in the skin was measured after 24 hours exposure.
The total amount that has penetrated the skin is the sum of amounts found in the skin and receptor compartment.
After 24 hr the receptor fluid found the amount of caffeine from oil in water emulsion more than water in oil
emulsion that mean caffeine absorbed through skin and flow in the blood stream that not still in the skin for reduce
cellulite in the subcutaneous layer. Whatever the vehicle used, the necessary time to reach a steady state was long,
ranging from 16 to 18 h and massaging so water in oil emulsion should be considered for cosmetic formulation
and preparation.
Acknowledgement
The study was grant supported by graduate school, prince of Songkla University.
References
Adolf,K. and. Kurt,W. (2005). Cosmetics design/ Formulation and Science. In Andre,OB., Marc,P., and
Howard,I.M.
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Bolzinger, M. A., Briancon, S., Pelletier, J., Fessi, H. and Chevalier, Y. (2008). Percutaneous release of caffeine
from microemulsion, emulsion and gel dosage forms.
Eur. J. Pharm. & Biopharm
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Potard,G., Laugel,C., Baillet,A., Schaefer,H., and Marty,J.P. (1999). Quantitative HPLC analysis of sunscreens
and caffeine during in vitro percutaneous penetration studies.
Int.J.Pharm
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