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3.2) „µ¦š—°„µ¦¥n°¥Å ¤´ œ
œÎµÂ‡š¸ Á¦¸¥„¦—¨„˜· „š¸É‡´—Á¨º°„Å—o š¸É Á¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Á®¨ª MRS Áž} œÁª¨µ 24
´ÉªÃ¤Š ¤µ ¸ —œ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°š¸ÉÁ˜·¤ 1% Tributyrin ¨o ªœÎµÅžn¤š¸É°»–®£¼¤· 37 °C Áž}œÁª¨µ 48-72 ´ÉªÃ¤Š ™o µ¤¸
„µ¦ÄoÅ ¤´œ‹³Á„·—ªŠÄ¦°Ç Ç蜸 (Michael and Pelezar, 1995)
3.3) „µ¦š—°„µ¦¥n°¥ÂžjŠ
œÎµÂ‡š¸ Á¦¸¥„¦—¨„˜· „š¸É‡´—Á¨º °„Å—oš¸É Á¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Á®¨ª MRS Áž} œÁª¨µ 24
´ÉªÃ¤Š ¤µ ¸—œ°µ®µ¦Á¨¸Ê¥ŠÁºÊ° starch œÎµÅžn¤š¸É°»–®£¼¤· 37 °C Áž} œÁª¨µ 48-72 ´ÉªÃ¤Š š—°„µ¦¥n°¥Âžj ŠÃ—¥
®¥—µ¦¨³¨µ¥Å°Ã°—¸œ ™oµ¤¸„µ¦¥n°¥¸ °Š°µ®µ¦Á¨¸Ê¥ŠÁºÊ°‹³Å¤nÁž¨¸É¥œÁž}œ¸œÊεÁŠ·œ (Michael and Pelezar, 1995)
4. „µ¦š—°ÂÂŸœ‡ªµ¤Åª °ŠÁºÊ°˜n°¥µž’·¸ªœ³
œÎµÂ‡š¸Á¦¸ ¥„¦—¨„˜·„š¸É‡´—Á¨º°„Å—oÁ¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê ¥ŠÁºÊ°Á®¨ª MRS Áž}œÁª¨µ 24 ´É ªÃ¤Š ¸—œ
°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Â È Š MRS nŠÁºÊ°Áž} œ 2 »— ‡º° »—š¸É 1 Á„ȝĜ anaerobic jar n¤š¸É 37 °C Áž}œÁª¨µ 24 ´ÉªÃ¤Š »—š¸É 2
n ¤š¸É 37 °C £µ¥Ä˜o £µª³š¸É¤¸°µ„µ«Áž}œÁª¨µ 24 ´ÉªÃ¤Š ˜¦ª‹—¼„µ¦Á‹¦· °ŠÁºÊ°œŸ·ª®œoµ°µ®µ¦š´Ê Š 2 £µª³
5. „µ¦š—°ž¦³·š›·£µ¡„µ¦¥´ ¥´ÊŠ„µ¦Á‹¦·Ã—¥¥µž’·¸ªœ³
čo cotton swab ™nµ¥Â‡š¸ Á¦¸¥„¦—¨„˜·„š¸É‡´—Á¨º°„Å—oš¸ÉÁ¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ °Á®¨ª MRS Áž}œÁª¨µ 18
´ÉªÃ¤Š ¨oªšÎµ„µ¦ž¦´Ä®o ¤¸‡ªµ¤ »nœÁž}œ 0.5 McFarland Ä®o ¤¸‹ÎµœªœÁºÊ° 108 CFU/ml œÎµÅžžjµ¥ (swab) Ä®oš´É ª
Ÿ·ª®œoµ °Š°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Â Ȋ MRS ¨oªž¨n°¥Ä®o®oŠ 3-5 œµš¸ ‹µ„œ´Ê œªµŠÂŸnœ¥µ¤µ˜¦“µœ (antibiotic discs)
‹Îµœªœ 15 œ·— ŗo„n penicillin G (10 ôg), ampicillin (10 ôg), cephalothin (30 ôg), cefoperazone (75 ôg),
vancomycin (30 ôg) bacitracin (10 ôg), gentamicin (10 ôg), kanamycin (30 ôg), streptomycin (10 ôg), tetra-
cycline (30 ôg), chloramphenicol (30 ôg), erythromycin (15 ôg), norfloxacin (10 ôg) ¨³ polymyxin B (300 ôg)
ªµŠ¨Šœ¦·Áª–š¸Éžj µ¥ÁºÊ°Åªo (šÎµ 2 ŽÊε) n¤ÁºÊ°š¸É°»–®£¼¤· 37 °C Áž}œÁª¨µ 24 ´ÉªÃ¤Š ¦µ¥ŠµœŸ¨Ã—¥„µ¦ª´ —ÁoœŸnµœ
«¼œ¥r„¨µŠ °ŠªŠÄ (inhibition zone) ¨oªœÎ µŸ¨š¸Éŗo ¤µÁž¦¸¥Áš¸¥Á¡ºÉ°—¼ªnµÁºÊ °—´Š„¨nµª Ū (sensitive) ®¦º°Ÿ¨„Êε„¹ÉŠ
(intermediate) ®¦º°˜oµœšµœ (resistant) ˜n°¥µž’· ¸ªœ³œ´ÊœÇ
6. „µ¦š—°‡ªµ¤µ¤µ¦™ °ŠÂ‡š¸Á¦¸ ¥„¦—¨„˜·„Äœ„µ¦¥´¥´ÊŠÁºÊ°Â‡š¸Á¦¸¥nŠ¸ÊĜ£µª³š¸ÉŤn ‹Îµ„´ —
„¦—°· œš¦¸¥r¨³Å±Ã—¦Á‹œÁž°¦r°°„ÅŽ—r ×¥ª·›¸ agar well diffusion
š—°„µ¦¥´¥´ÊŠÃ—¥ª·›¸ agar well diffusion ×¥œÎ µÂ‡š¸Á¦¸¥n Š¸Ê Staphylococcus aureus TISTR 517
¨³ Escherichia coli TISTR 887 ¤µÁ¨¸Ê¥ŠÄœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Á®¨ª LB n¤ÁºÊ°š¸É°»–®£¼¤· 37 °C Áž}œÁª¨µ 24 ´ÉªÃ¤Š
œÎ µÅžžjµ¥ (swab) Ä®o š´ÉªŸ·ª®œo µ °Š°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Â Ȋ MHA ¨oªž¨n°¥Ä®o®o Šš·ÊŠÅªo 3-5 œµš¸ ‹µ„œ´ÊœÁ‹µ³ª»o œ
°µ®µ¦Ã—¥Äo ž¨µ¥š·žÂ¨³Á ¸É ¥ª»oœ°°„ œÎ µœÊεÁ¨¸Ê¥ŠÁºÊ °Â‡š¸Á¦¸¥„¦—¨„˜·„š¸Én ¤ÁºÊ°Åªo 24 ´ÉªÃ¤Š 80 ŤÇ¦¨·˜¦
®¥—¨ŠÄœ®¨»¤š¸ÉÁ‹µ³Åªoœ°µ®µ¦Á¨¸Ê¥ŠÁºÊ °Â Ȋ MRS ˜´Ê Šš·ÊŠÅªo Ä®o®oŠÂ¨oªœÎµÅžn¤ÁºÊ°š¸É°»–®£¼¤· 37 °C Áž}œÁª¨µ 24
´ÉªÃ¤Š ˜¦ª‹Ÿ¨„µ¦¥´¥´ÊŠÃ—¥—¼ ‹µ„ªŠÄ °Š„µ¦¥´¥´Ê Š (inhibition zone) (—´—ž¨Š‹µ„ Spelhaug and Harlander,
1989)
Ÿ¨„µ¦ª·‹´¥Â¨³°£· ž¦µ¥Ÿ¨
„µ¦Â¥„ÁºÊ°Â‡š¸ Á¦¸¥„¦—¨„˜·„Ä®oÁž}œÁºÊ°š¸É¦· »š›·Í
‡š¸Á¦¸¥„¦—¨„˜·„š¸ÉÂ¥„Å—o ‹µ„˜Š„ªµÄ®oÁž}œÁºÊ °¦· »š›·Í‹µ„×¥ª· ›¸„µ¦ ¸—ÁºÊ°œ°µ®µ¦Á¨¸Ê¥ŠÁºÊ°Â Ȋ
MRS š¸ÉÁ˜·¤ bromocresol purple 0.004% ŗo Áž}œÃ‡Ã¨œ¸¤¸¨´„¬–³š¸É ˜„˜nµŠ„´œ Ánœ Ç蜸„¨¤ °Á¦¸¥®¦º°®¥´„