full2010.pdf - page 1942

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ª·Á‡¦µ³®r Þ¦˜¸œ Á‡¦ºÉ°Šª·Á‡¦µ³®r Å ¤´œ
°µ®µ¦Á¨¸Ê¥ŠÁºÊ° ŗo„n MR-VP broth, Plate count agar, Bacillus cereus selective supplement, Blood
agar (base), Potato dextrose agar, Bacillus cereus selective agar base (MYP), Tryptic soy broth, Egg yolk
emulsion 50%, Phenol-red broth (base), Nutrient agar, Nutrient broth, Fuchsin for microscopy certistain, BAIRD-
PARKER agar, Pyruvic acid sodium salt, Brain heart-Infusion broth (BHI), Bactident Coagulase Rabbit plasma
with EDTA, Egg yolk tellurite emulsion 20 %, ¨³ Lauryl sulfate broth
µ¦Á‡¤¸š¸É čoĜ„µ¦ª·Á‡¦µ³®r Þ¦˜¸œ ‡µ¦rÝűÁ—¦˜ Å ¤´œ Á„¨º°Â¦n Áž}œœ·— Analytical grade
ª´ —» ŗo„n oµª„¨o°Š´Š r®¥— œÊ嘵¨š¦µ¥ žjŠ oµªÁ‹o µ ¤³¡¦oµª ‹»¨·œš¦¸¥rÁž¦¸ ¥Áš¸¥ ŗo „n
Bacillus cereus TISTR 687, Staphylococcus aureus TISTR 118 ¨³ Escherichia coli TISTR 780
ª· ›¸„µ¦—εÁœ·œ„µ¦š—¨°Š
1. „µ¦Á˜¦¸¥¤ÂžjŠ oµª„¨o°Š´Š r®¥—
œÎ µ oµª„¨o°Š´Š r®¥—‹µ„°ÎµÁ£°µŠÂ„oª ‹´ Š®ª´—¡´š¨»Š œÊÎ µ®œ´„ 10 „·Ã¨„¦´¤ ¤µ—änÂ®oŠ—oª¥
Á‡¦ºÉ°ŠÃ¤n— (Sottorriva, Italy) ¦»nœ MZ „ε¨´Š °Š¤°Á˜°¦r 1.1 ¦Š¤oµ ‡ªµ¤™¸É 50 Á±·¦rš ‡ªµ¤˜nµŠ«´„¥r 220 ꨘr
2. «¹„¬µ°Š‡rž¦³„° °ŠÂžj Š oµª„¨o°Š´Š r®¥—
ª·Á‡¦µ³®r °Š‡rž¦³„°šµŠÁ‡¤¸ ŗo„n Þ¦˜¸œ Å ¤´œ Á™oµ ÁoœÄ¥ ‡µ¦rÝűÁ—¦˜ čoª·›¸š—°˜µ¤
AOAC (1999) ‡¨ÁŽ¸¥¤ Á®¨È„ ¤Š„µœ¸ ¤„œ¸ÁŽ¸¥¤ ޚÁŽ¸¥¤ ´Š„³¸ —o ª¥ª·›¸ Atomic Absorption
Spectroscopy ¢°¢°¦´ —oª¥ª·›¸ Wet digest ¨³ Molybdenum blue method «¹„¬µ‡»–¤´˜·šµŠ„µ¥£µ¡Â¨³
¸ª£µ¡ ŗo„n ¡¸Á° ž¦·¤µ–‡ªµ¤ºÊ œ —oª¥ª·›¸ Gravimetric method ÁºÊ°‹»¨·œš¦¸¥rš´ÊŠ®¤— ¥¸ ˜r¦µ Bacillus cereus,
Staphylococcus aureus ¨³ Escherichia coli čoª·›¸š—°˜µ¤ AOAC (1999)
3. „¦³ªœ„µ¦Âž¦¦¼žÂžjŠ oµª´Š r®¥—
3.1 „µ¦šÎµ œ¤‹¸œ
3.1.1 „µ¦°°„¼˜¦ œ¤‹¸ œ ×¥„µ¦ªµŠÂŸœ„µ¦š—¨°ŠÂ Simplex-Lattice Mixture
Design ŗoš´Ê Š®¤— 10 ¼˜¦ —´Š˜µ¦µŠš¸É 1