970
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ĕúđðÿÝćÖéĉ
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Screening of Lipase Producing Bacteria from Soil and Fermented Fish Production
Wastewater
ÿĉ
øĉ
îćëǰðúĂéÿč
üøøè
1*
Ēúąÿč
ßĆ
ââćǰìĂÜđÙøČ
Ă
2
Sirinart Plodsuwan
1*
and Suchanya Thongkrua
2
ïìÙĆ
é÷Š
Ă
Üćîüĉ
ÝĆ
÷îĊĚ
öĊ
üĆ
êëč
ðøąÿÜÙŤ
đóČę
ĂÙĆ
éđúČ
ĂÖĒïÙìĊ
đøĊ
÷ìĊę
ÿćöćøëñúĉ
êđĂîĕàöŤ
ĕúđðÿÝćÖéĉ
îĒúąîĚĞ
ćđÿĊ
÷Öćøñúĉ
êðúćÿš
öǰ
ĔîóČĚ
îìĊę
ÝĆ
ÜĀüĆ
éóąđ÷ćǰēé÷ÙĆ
éĒ÷ÖÝćÖĒïÙìĊ
đøĊ
÷ìĊę
ÿćöćøëđðúĊę
÷îÿĊ
bromocresol purple ĔîÝćîĂćĀćøǰscreening
mediumǰÝćÖÿĊ
öŠ
üÜđðŨ
îÿĊ
đĀúČ
ĂÜǰïŠ
öìĊę
Ăč
èĀõĎ
öĉ
ǰ30ǰĂÜýćđàúđàĊ
÷ÿǰđðŨ
îđüúćǰ48 ßĆę
üēöÜǰóïĒïÙìĊ
đøĊ
÷ìĊę
ÙĆ
éĒ÷Öĕéš
ìĆĚ
ÜĀöéǰ
14ǰĕĂēàđúìǰàċę
Üĕéš
ÝćÖéĉ
îǰ1 ĕĂēàđúìǰDS1ǰ÷š
Ăöêĉ
éÿĊ
ĒÖøöïüÖǰøĎ
ðøŠ
ćÜÖúö đøĊ
÷ÜêĆ
üđðŨ
îÖúčŠ
öÙúš
ć÷óüÜĂÜčŠ
îǰĒúąĕéš
ÝćÖîĚĞ
ćđÿĊ
÷ǰ
13ǰĕĂēàđúìǰWS1-WS13ǰÿŠ
üîĔĀâŠ
÷š
Ăöêĉ
éÿĊ
ĒÖøöúïǰøĎ
ðøŠ
ćÜìŠ
ĂîÿĆĚ
îǰđøĊ
÷ÜêĆ
üÖøąÝĆ
éÖøąÝć÷ǰđöČę
ĂîĞ
ćöćüĉ
đÙøćąĀŤ
ÙŠ
ćéĆ
ßîĊ
đĂîĕàöŤ
ĕúđðÿǰóïüŠ
ćĕĂēàđúìǰDS1, WS10ǰĒúą WS6 öĊ
ÙŠ
ćéĆ
ßîĊ
đĂîĕàöŤ
ĕúđðÿđìŠ
ćÖĆ
ï 2.80, 2.66 Ēúą 2.64ǰêćöúĞ
ćéĆ
ï
ĒúąÖćøüĉ
đÙøćąĀŤ
ÙŠ
ćÖĉ
ÝÖøøöđĂîĕàöŤ
ĕúđðÿ óïüŠ
ćĕĂēàđúìǰDS1, WS12ǰĒúą WS13ǰöĊ
ÙŠ
ćÖĉ
ÝÖøøöđĂîĕàöŤ
ĕúđðÿđìŠ
ćÖĆ
ïǰ
95.45, 93.18 Ēúą 93.18
ĀîŠ
ü÷êŠ
Ăöĉ
úúĉ
úĉ
êø
êćöúĞ
ćéĆ
ï
ÙĞ
ćÿĞ
ćÙĆ
â:
ĒïÙìĊ
đøĊ
÷ǰđĂîĕàöŤ
ĕúđðÿǰîĚĞ
ćđÿĊ
÷Öćøñúĉ
êðúćÿš
ö
Abstract
The objective of this research was to screen lipase producing bacteria from soil and fermented
fish production wastewater in Phayao province. Bacteria, that can change color of bromocresol purple
in screening medium from purple to yellow, were isolated and incubated at 30 °C for 48 hours. Fourteen
isolates were isolated. Isolate DS1 was from soil, Gram positive, cocci shapes and clusters. Amoung
these, 13 isolates (WS1 - WS13) were obtained from wastewater, Gram negative, rod shape and singles.
Lipase enzyme index of isolated bacteria were investigated. DS1, WS10 and WS6 had lipase enzyme
index as 2.80, 2.66 and 2.64, respectively. Lipase activity of DS1, WS12 and WS13 were 95.45, 93.18 and
93.18 units/mL, respectively.
Keywords:
Bacteria, Lipase Enzyme, Fermented Fish Production Wastewater
_________________________________
1
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êðøĉ
ââćēìǰÿć×ćüĉ
ßćüĉ
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ÿĉę
ÜĒüéúš
Ăöǰüĉ
ì÷ćúĆ
÷óúĆ
ÜÜćîĒúąÿĉę
ÜĒüéúš
ĂöǰöĀćüĉ
ì÷ćúĆ
÷óąđ÷ćǰ56000
2
Ă éø , ÿć×ćüĉ
ßćüĉ
ì÷ćýćÿêøŤ
ÿĉę
ÜĒüéúš
Ăöǰüĉ
ì÷ćúĆ
÷óúĆ
ÜÜćîĒúąÿĉę
ÜĒüéúš
ĂöǰöĀćüĉ
ì÷ćúĆ
÷óąđ÷ćǰ56000
*Corresponding author: Tel.: 087-8898993. E-mail address:
