962
P39
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ÿćöćøëìîêŠ
ĂÙüćöđðŨ
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÷ēøÜÜćîôĂÖĀîĆ
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Isolation of Lead Toxicity Resistant Bacteria from Soil and Tannery Wastewater
Ăõĉ
ßâćǰÖćÿč
÷ą
1*
Ēúąÿč
ßĆ
ââćǰìĂÜđÙøČ
Ă
2
Apichaya Kasuya
1*
and Suchanya Thongkrua
2
ïìÙĆ
é÷Š
Ă
Üćîüĉ
ÝĆ
÷îĊĚ
öĊ
üĆ
êëč
ðøąÿÜÙŤ
đóČę
ĂÙĆ
éĒ÷ÖĒïÙìĊ
đøĊ
÷ìĊę
ÿćöćøëìîêŠ
ĂÙüćöđðŨ
îóĉ
þ×ĂÜêąÖĆę
üÝćÖéĉ
îǰĒúąîĚĞ
ćđÿĊ
÷ēøÜÜćî
ôĂÖĀîĆ
Üǰēé÷ìĞ
ćÖćøÙĆ
éĒ÷ÖĒïÙìĊ
đøĊ
÷ìĊę
đÝøĉ
âïîÝćîĂćĀćøǰnutrient agarǰìĊę
öĊ
ǰlead acetate ïŠ
öìĊę
ǰ37 ĂÜýćđàúđàĊ
÷ÿǰ
đðŨ
îđüúćǰ24 ßĆę
üēöÜǰóïüŠ
ćĒïÙìĊ
đøĊ
÷ìĊę
ÙĆ
éĒ÷Öĕéš
ÝćÖéĉ
îöĊ
ìĆĚ
ÜĀöéǰ6 ĕĂēàđúìǰ PBS1-PBS6 ǰĒúąÝćÖîĚĞ
ćđÿĊ
÷ēøÜÜćîôĂÖ
ĀîĆ
ÜöĊ
ìĆĚ
ÜĀöéǰ10 ĕĂēàđúìǰ PBW1-PBW10 ǰđöČę
ĂîĞ
ćĕðìéÿĂïÖćøìîêŠ
ĂÙüćöđðŨ
îóĉ
þ×ĂÜêąÖĆę
üǰ Minimal Inhibitory
Concentration: MIC ǰìĊę
Ùüćöđך
öך
î×ĂÜǰlead acetate đìŠ
ćÖĆ
ïǰ500-1300 öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êøǰéš
ü÷đìÙîĉ
Ùǰdrop plate
ïîÝćîĂćĀćøǰnutrient agar ïŠ
öìĊę
ǰ37 ĂÜýćđàúđàĊ
÷ÿǰđðŨ
îđüúćǰ24 ßĆę
üēöÜǰóïüŠ
ćĒïÙìĊ
đøĊ
÷ìĊę
ÙĆ
éĒ÷Öĕéš
ÝćÖéĉ
îǰÙČ
ĂǰPBS1
öĊ
ÙŠ
ćǰMIC đìŠ
ćÖĆ
ïǰ1250 öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êøǰĒúąǰPBS2-PBS6 öĊ
ÙŠ
ćǰMIC đìŠ
ćÖĆ
ïǰ1300 öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êøǰĒïÙìĊ
đøĊ
÷ìĊę
ÙĆ
éĒ÷Ö
ĕéš
ÝćÖîĚĞ
ćđÿĊ
÷ēøÜÜćîôĂÖĀîĆ
ÜǰÙČ
ĂǰPBW4 öĊ
ÙŠ
ćǰMIC đìŠ
ćÖĆ
ï 500 öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êøǰPBW2 öĊ
ÙŠ
ćǰMIC đìŠ
ćÖĆ
ïǰ1150
öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êøǰĒúąǰPBW1, PBW3, PBW5-PBW10 öĊ
ÙŠ
ćǰMIC đìŠ
ćÖĆ
ïǰ1300 öĉ
úúĉ
ÖøĆ
öêŠ
Ăúĉ
êø
ÙĞ
ćÿĞ
ćÙĆ
â:
ĒïÙìĊ
đøĊ
÷ǰêąÖĆę
üǰîĚĞ
ćđÿĊ
÷ēøÜÜćîôĂÖĀîĆ
Ü
Abstract
The aim of this research was to isolate lead toxicity resistant bacteria from soil and tannery
wastewater. Lead toxicity resistant bacteria were isolated on nutrient agar containing lead acetate,
incubated at 37 °C for 24 hours. Six bacterial strains were isolated from soil (PBS1-PBS6) and 10 isolates
obtained from tannery wastewater (PBW1-PBW10). Minimal Inhibitory Concentration MIC ǰat
concentration of lead as 500-1300 mg/L were studied by drop plate technique on nutrient agar,
incubated at 37 °C for 24 hours. PBS1 had MIC at 1250 mg/L and PBS2-PBS6 had MIC at 1300 mg/L.
Lead resistant bacteria from tannery wastewater as PBW4 had MIC at 500 mg/L, PBW2 had MIC at
1150 mg/L and MIC of PBW1, PBW3, PBW5-PBW10 had MIC at 1300 mg/L.
Keywords:
Bacteria, Lead, Tannery wastewater
1
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ÜĒüéúš
Ăöǰüĉ
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÷óúĆ
ÜÜćîĒúąÿĉę
ÜĒüéúš
ĂöǰöĀćüĉ
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÷óąđ÷ćǰóąđ÷ćǰ56000
2
Ă.éø.,ǰÿć×ćüĉ
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ÜĒüéúš
Ăöǰüĉ
ì÷ćúĆ
÷óúĆ
ÜÜćîĒúąÿĉę
ÜĒüéúš
ĂöǰöĀćüĉ
ì÷ćúĆ
÷óąđ÷ćǰóąđ÷ćǰ56000
*
Corresponding author: Tel.: 088-2613565. E-mail address:
