954
P38
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Isolation of Lipase-Producing Bacteria from Soil and Restaurant Wastewater
Contaminated with Fat, Oil and Grease
öćøĉ
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Ă
2
Marisa Attapong
1 ǰǰ
and Suchanya Thongkrua
2
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é÷Š
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Öćøýċ
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ðøąÿÜÙŤ
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éĒ÷ÖĒïÙìĊ
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ÿćöćøëñúĉ
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ðîđðŚŪ
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îĒúąĕ×öĆ
îǰēé÷ÙĆ
éĒ÷ÖĒïÙìĊ
đøĊ
÷ïîĂćĀćøǰscreening medium ïŠ
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Ăč
èĀõĎ
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ǰ 7ǰĂÜýćđàúđàĊ
÷ÿ đðŨ
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đüúćǰ ǰßĆę
üēöÜǰÿćöćøëÙĆ
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ÜĀöéǰ ǰĕĂēàđúìǰàċę
Üĕéš
ÝćÖéĉ
îǰ ǰĕĂēàđúìǰĕéš
ĒÖŠ
SLP1 ĒúąǰSLP2ǰĒúą
ÝćÖîĚĞ
ćđÿĊ
÷ǰ ǰĕĂēàđúìǰĕéš
ĒÖŠ
WLP1 ëċ
Ü WLP26ǰúĆ
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ì÷ć×ĂÜĒïÙìĊ
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ĂîÿĆĚ
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Ăî÷ćüǰÝćÖîĆĚ
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ÙüćöÿćöćøëĔîÖćøñúĉ
êđĂîĕàöŤ
ĕúđðÿǰēé÷ÖćøéĎ
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đüèĔÿïîĂćĀćøǰtributyrin agar ïŠ
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Ăč
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ǰ 7ǰĂÜýćđàúđàĊ
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đðŨ
îđüúćǰ48 ßĆę
üēöÜ óïüŠ
ć WLP26,ǰWLP1, SLP2, WLP7ǰĒúąǰWLP5 öĊ
ÙŠ
ćéĆ
ßîĊ
đĂîĕàöŤ
ĕúđðÿđìŠ
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ï
2.32, 2.22, 2.06, 1.86
Ēúąǰ1.85
êćöúĞ
ćéĆ
ï
Öćøüĉ
đÙøćąĀŤ
Öĉ
ÝÖøøöđĂîĕàöŤ
ĕúđðÿǰóïüŠ
ćǰWLP20, WLP1, WLP21, WLP5 Ēúą WLP26ǰöĊ
ÙŠ
ć
Öĉ
ÝÖøøöđĂîĕàöŤ
ĕúđðÿđìŠ
ćÖĆ
ïǰ182.43, 175.67, 168.92, 166.67 Ēúąǰ159.90 ĀîŠ
ü÷êŠ
Ăöĉ
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êø êćöúĞ
ćéĆ
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ÙĞ
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â:
ĒïÙìĊ
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÷ǰđĂîĕàöŤ
ĕúđðÿ îĚĞ
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÷ îĚĞ
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Abstract
The purpose of this study was to isolate lipase-producing bacteria from soil and restaurant
wastewater contaminated with fat, oil and grease. Bacteria were isolated on screening medium and
JODVCBUFEǰBUǰ ǰÑ$ǰfor 24 hours. Twenty eight bacterial strains were isolated. Amoung these, 2 strains
(SLP1 and SLP2) were isolated from soil and 26 isolates (WLP1 to WLP26) were obtained from
wastewater. Morphology of almost isolated bacterial strains from soil and wastewater were Gram
negative, rod shape and Gram positive, cocci and rod shape. Capacity of lipase enzyme production
GSPNǰJTPMBUFEǰTUSBJOTǰXFSFǰPCTFSWFEǰPGǰDMFBSǰ[POFǰPOǰUSJCVUZSJOǰBHBS ǰJODVCBUFEǰBUǰ ǰÑ$ǰfor 48 hours.
Strain WLP26,ǰWLP1, SLP2, WLP7ǰandǰWLP5 had lipase enzyme index as 2.32, 2.22, 2.06, 1.86 andǰ1.85,
respectively. Lipase activity of strain WLP20, WLP1, WLP21, WLP5 and WLP26 were 182.43, 175.67,
168.92, 166.67 andǰ159.90 units/mL, respectively.
Keywords:
Bacteria,ǰLipase Enzyme, Wastewater, Fat, Oil and Grease
1
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*
Corresponding author: Tel.: 087-0842612. E-mail address:
