เอกสารการประชุมวิชาการและเสนอผลงานวิจัย มหาวิทยาลัยทักษิณ ครั้งที่ 19 2552 - page 1257

5
„µ¦ž¦³»¤ª·µ„µ¦Â¨³Áœ°Ÿ¨Šµœª·‹´¥¤®µª·š¥µ¨´¥š´ „¬·– ‡¦´Ê Šš¸É 19 ž¦³‹Îµže 2552
‡¦´ÊŠš¸É
œÊÎ µ®œ´„Áž¨º°„¤³œµªš¸É
čoĜ„µ¦„´—
(„·Ã¨„¦´¤)
„¦—š¸É čož¦´ 
pH pH
Áª¨µš¸Éčo
Ĝ„µ¦„´—
(œµš¸)
Áž‡˜·œš¸É
„´—Å—o
(„¦´ ¤)
Áž‡˜·œš¸É „´—Å—o
‡·—Áž}œ¦o°¥¨³
¦o°¥¨³
ÁŒ¨¸É¥
1 3.5
10%HCl
3.05
30
72.10 2.06 2.06±0.0
8 2 3.8 3.16 81.32 2.14
3 3.5 3.09 69.65 1.99
1 3.6 3.07
60
84.60 2.35 2.18±0.2
5 2 4.1 3.12 93.89 2.29
3 4.5 3.11 85.05 1.89
1 3.5
10%H2SO4
3.12
30
62.65 1.79 1.88±0.1
1 2 3.5 3.25 64.75 1.85
3 3.6 3.11 72.36 2.01
1 3.7 3.05
60
82.14 2.22 2.03±0.1
9 2 4.1 3.01 82.82 2.02
3 3.9 3.18 72.15 1.85
1 3.5
10%H3PO4
3.26
60
65.80 1.88 1.85±0.0
6 2 3.5 3.11 66.15 1.89
3 4.1 3.01 73.39 1.79
Áž‡˜·œ‹µ„š»„„µ¦Ÿ¨·˜—oª¥ž{‹‹´¥˜nµŠÇĜ˜µ¦µŠš¸É 1 Á¤ºÉ°œÎµÅžš—°¤´ ˜·˜nµŠÇ ŗo„n „µ¦š—°
‡µ¦r ÝűÁ—¦˜ (Ferric ChlorideTest, Seliwanoff’s test ¨³ Iodine test), „µ¦š—°Ã¡¨¸ÂŽ‡‡µÅ¦—r, „µ¦š—°
µ¦Áž‡˜·œ, „µ¦š—° reducing sugar, „µ¦ª·Á‡¦µ³®r methoxy content ¨³„µ¦®µ Loss on drying ¡ªn µÅ—oŸ¨
Ą¨o Á‡¸¥Š„´œ —´ŠÂ—ŠÄœ˜µ¦µŠš¸É 2
˜µ¦µŠš¸É 2 Ÿ¨„µ¦«¹„¬µ¤´˜·šµŠÁ‡¤¸ °Šµ¦„´—Áž‡˜·œ‹µ„Áž¨º °„¤³œµªš»„ž{‹‹´¥
„µ¦š—° ª·›¸ „µ¦š—° Ÿ¨„µ¦š—°
„µ¦š—°‡µ¦rÝűÁ—¦˜ Ferric ChlorideTest Á„·—Áž}œ˜³„°œª»oœ
Seliwanoff’s test µ¦¨³¨µ¥¸œÊ嘵¨Ä
Iodine test µ¦¨³¨µ¥¸œÊ嘵¨Ä
„µ¦š—°Ã¡¨¸ÂŽ‡‡µÅ¦—r lead acetate solution Á„·—Áž}œ˜³„°œª»oœ
„µ¦š—°µ¦Áž‡˜·œ Á˜·¤ 95% alcohol Á„·—Áž}œ˜³„°œª»oœ
Á˜·¤ 2 N NaOH Á„·— gel
„µ¦š—° reducing sugar Fehling’s test µ¦¨³¨µ¥¸œÊεÁŠ·œ
„µ¦ª·Á‡¦µ³®r methoxy content (%) USP standard 24.42 ± 4.08 (n = 9)
„µ¦®µ Loss on drying (%) USP standard 5.62 ± 1.06 (n = 9)
„µ¦˜¦ª‹®µÃ¨®³Â¨³µ¦®œ¼Äœµ¦
„´—Áž‡˜· œ
˜µ¤ª· ›¸°ŠÁ£´˜Îµ¦´ °Š
®¦´ “°Á¤¦·„µ
Ťn¡
‹µ„Ÿ¨„µ¦š—¨°Š˜µ¦µŠš¸É 2 —Šªnµµ¦„´ —Áž‡˜·œ‹µ„Áž¨º°„¤³œµª—oª¥Á‡¦ºÉ°Š¤º °—´—ž¨Šš¸É
‹´—šÎ µ ¹Êœ š»„£µª³„µ¦Ÿ¨·˜Ÿnµœ„µ¦˜¦ª‹°¤µ˜¦“µœ˜µ¤Á£´ ˜Îµ¦´ °Š®¦´“°Á¤¦·„µ ×¥Áž‡˜· œš¸ÉŗoÁž}œ
‡µ¦r ÝűÁ—¦˜„¨»n¤ Polyuronide œ°„‹µ„œ´ÊœÅ—oª·Á‡¦µ³®rÁž‡˜·œš¸É „´— ×¥ª·›¸ FTIR —oª¥Á‡¦ºÉ°Š Equinox 55, Bruker
š¸É nªŠ‡ªµ¤™¸É 4000-400 cm-1 ¡ªnµÅ—o spectrogram °ŠÁž‡˜·œš¸É „´—‹µ„Áž¨º °„¤³œµª¤¸¨´„¬–³Án œÁ—¸¥ª„´Áž‡
˜·œšµŠ„µ¦‡oµ —´Š¦¼žš¸É 2 ¨³Ÿ¨„µ¦š—¨°ŠÁž}œÁn œÁ—¸¥ª„´ „µ¦«¹„¬µ °Š Tamaki ¨³‡–³ (2008) „µ¦š—°—oª¥