991
P43
Ùüćöÿćöćøë×ĂÜøćđĂîēéĕôìŤ
ĔîÖćøñúĉ
êđĂîĕàöŤ
đàúúĎ
đúÿĒúąÿćøêš
ćîđßČĚ
Ăøć
The Capability of Endophytic Fungi for Production of Enzyme Cellulase and Antifungal
substances
đÖýÖîÖǰđ×Ċ
÷üĂč
éö
1
Ēúąǰóùìåĉ
õøǰýč
õóú
2*
Kedkanok Keawudom
1
and Preuttiporn Supaphon
2*
ïìÙĆ
é÷Š
Ă
Üćîüĉ
ÝĆ
÷îĊĚ
êš
ĂÜÖćøÿĞ
ćøüÝÙüćöÿćöćøë×ĂÜøćđĂîēéĕôìŤ
ìĊę
Ē÷Öĕéš
ÝćÖðćúŤ
öîĚĞ
ćöĆ
îđóČę
ĂĔßš
ÙüïÙč
öđßČĚ
ĂÖŠ
ĂēøÙóČ
ßǰ
1IZUPQIUIPSB
ǰ
QBMNJWPSBǰ
Ēúąǰ
1IZUPQIUIPSBǰ CPUSZPTB
ǰ ēé÷Ĕßš
üĉ
íĊ
ǰdual culture ĒúąýĆ
Ö÷õćóĔîÖćøñúĉ
êđĂîĕàöŤ
đàúúĎ
đúÿ ĔîÖćøÙĆ
éđúČ
ĂÖÿćöćøëĒ÷ÖøćđĂîēéĕôìŤ
ĕéš
ìĆĚ
ÜĀöéǰ ǰĕĂēàđúì ĒúąÝĆ
éÝĞ
ćĒîÖēé÷úĆ
ÖþèąìćÜÿĆ
èåćîüĉ
ì÷ćǰÿćöćøëÝĆ
é
ÝĞ
ćĒîÖøćđĂîēéĕôìŤ
ĕéš
đóĊ
÷Üǰ ǰĕĂēàđúìǰ L1C02 ǰđðŨ
îǰ
1FTUBMPUJPQTJTǰ
sp ǰîĞ
ćđßČĚ
ĂìĆĚ
ÜĀöéìĊę
Ē÷Öĕéš
öćìéÿĂïÖćø÷Ć
ï÷ĆĚ
Ü
ǰ 1 ǰ
QBMNJWPSB
ǰĒúąǰ
1 ǰCPUSZPTB
ǰ öĊ
øćđĂîēéĕôìŤ
ǰ8 ĕĂēàđúìǰìĊę
÷Ć
ï÷ĆĚ
ÜđßČĚ
Ăǰ
1 ǰQBMNJWPSB
ǰĒúąöĊ
ǰ5 ĕĂēàđúìÿćöćøë÷Ć
ï÷ĆĚ
ÜđßČĚ
Ăǰ
1 ǰ
CPUSZPTB
ǰ ēé÷ĕĂēàđúìǰP3A02 (Unidentified fungi) ĔĀš
ñúÖćø÷Ć
ï÷ĆĚ
ÜéĊ
ìĊę
ÿč
éǰēé÷÷Ć
ï÷ĆĚ
Üǰǰ
1 ǰ QBMNJWPSB
(68.5%) Ēúąǰ
1 ǰ
CPUSZPTB
(69.7%)ǰǰĒúąöĊ
øćđĂîēéĕôìŤ
đóĊ
÷Üǰ ǰĕĂēàđúìǰP1A02ǰ unidentified fungi ǰđöČę
ĂîĞ
ćĕðìéÿĂïÖćøÿøš
ćÜđĂîĕàöŤ
đàúúĎ
đúÿǰïîĂćĀćøǰCMCǰĒÿéÜÙŠ
ćǰhydrolysis capacity (HC) đìŠ
ćÖĆ
ïǰ1.77
ÙĞ
ćÿĞ
ćÙĆ
â
: øćđĂîēéĕôìŤ
ǰùìíĉĝ
êš
ćîđßČĚ
ĂøćǰđĂîĕàöŤ
Abstract
This research aims to study the capability of endophytic fungi from oil palm against
1IZUPQIUIPSB
ǰ
QBMNJWPSB
ǰ
and
ǰ
1IZUPQIUIPSBǰCPUSZPTF
and their potential to produce cellulose. For selecting the antagonist
candidates against these plant pathogens, dual culture method was used for screening test. A total of 59
endophytic fungi were isolated from healthy oil palm
ǰ
and identified based on their morphological
characteristics. One isolates L1C02) was identified as
1FTUBMPUJPQTJT
sp. All isolates screened for antagonism
against fungal plant pathogens, 8 isolates against
1 ǰ QBMNJWPSBǰ
and 5
ǰ
isolates
ǰ
against
1 ǰ CPUSZPTB ǰ
ǰP3A02
(Unidentified fungi) showed a high antagonism effect to
1 ǰQBMNJWPSB
(68.5%) andǰ
1 ǰCPUSZPTB
(69.7%). Only
one isolate of endophytic fungi, unidentified endophytic fungi
ǰ
(P1A02) was tested cellulase production on
CMC agar and showed highest hydrolysis capacity (HC) as 1.77.
Keywords
: Endophytic fungi, Antifungal activities, Enzyme
1
îĉ
ÿĉ
êðøĉ
ââćêøĊ
ǰÿć×ćüĉ
ßćßĊ
üüĉ
ì÷ćǰÙèąüĉ
ì÷ćýćÿêøŤ
ǰöĀćüĉ
ì÷ćúĆ
÷ìĆ
Öþĉ
èǰóĆ
ìúč
Üǰ
ǰ
2
Ă éø ǰÿć×ćüĉ
ßćßĊ
üüĉ
ì÷ćǰÙèąüĉ
ì÷ćýćÿêøŤ
ǰöĀćüĉ
ì÷ćúĆ
÷ìĆ
Öþĉ
èǰóĆ
ìúč
Üǰ93210
*Corresponding author: Tel: 0620533388. E-mail :
