811
P18
ñú×ĂÜÿćøÿÖĆ
éêŠ
ĂÖćøđÖĘ
ïđÖĊę
÷üđĂîĕàöŤ
ēðøêĊ
đîÿ
ÝćÖöš
ćöðúćìĎ
îŠ
ćóĆ
îíčŤ
ÙøĊ
ï÷ćü (
5IVOOVTǰBMBMVOHB
)
Effect of Extraction Media on the Recovery of Proteinase
from Spleen of Albacore Tuna (
5IVOOVTǰBMBMVOHB
)
íĆ
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1
ǰĒúąÿøøóÿĉ
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ÖúŠ
ĂöđÖúš
ć
2*
Tanchanok Poonsin
1
and Sappasith Klomklao
2*
ïìÙĆ
é÷Š
Ă
Öćøýċ
Öþćñú×ĂÜÿćøÿÖĆ
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ĂÖćøđÖĘ
ïđÖĊę
÷üđĂîĕàöŤ
ēðøêĊ
đîÿÝćÖöš
ćöðúćìĎ
îŠ
ćóĆ
îíčŤ
ÙøĊ
ï÷ćü ǰ(
5IVOOVTǰ
BMBMVOHB
)
óïüŠ
ćßîĉ
é×ĂÜÿćøÿÖĆ
é Ùüćöđך
öך
î×ĂÜēàđéĊ
÷öÙúĂĕøéŤ
Ēúąßîĉ
é×ĂÜÿćøúéĒøÜêċ
Üñĉ
üöĊ
ñúêŠ
ĂÖćøÿÖĆ
é
đĂîĕàöŤ
ēðøêĊ
đîÿǰÖćøÿÖĆ
ééš
ü÷ÿćøúąúć÷ēàđéĊ
÷öôĂÿđôêïĆ
ôđôĂøŤ
ǰìĊę
øąéĆ
ïÙüćöđך
öך
îǰ50 öĉ
úúĉ
ēöúćøŤ
óĊ
đĂß 7ǰöĊ
ðøąÿĉ
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ïđÖĊę
÷üđĂîĕàöŤ
ēðøêĊ
đîÿÿĎ
ÜÖüŠ
ćîĚĞ
ćÖúĆę
îĒúąÿćøúąúć÷ǰTris-HCl ìĊę
øąéĆ
ïÙüćöđך
öך
î 50ǰöĉ
úúĉ
ēöúćøŤ
óĊ
đĂß 7 (p<0.05) đöČę
ĂĔßš
ÿćøúąúć÷ēàđéĊ
÷öôĂÿđôêïĆ
ôđôĂøŤ
ǰÙüćöđך
öך
î 50 öĉ
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ēöúćøŤ
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đĂß 7 ìĊę
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ēàđéĊ
÷öÙúĂĕøéŤ
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ïÙüćöđך
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îĒêÖêŠ
ćÜÖĆ
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éđĂîĕàöŤ
óïüŠ
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ĂÙüćöđך
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î×ĂÜēàđéĊ
÷öÙúĂĕøéŤ
đóĉę
ö×ċĚ
îǰ(0-1.25ǰēöúćøŤ
)
Öĉ
ÝÖøøö×ĂÜđĂîĕàöŤ
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î (p<0.05) ÿćøÿÖĆ
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îǰ1.25ǰēöúćøŤ
ǰÿĞ
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ïñú×ĂÜÿćøúéĒøÜêċ
Üñĉ
üóïüŠ
ćñúñúĉ
ê×ĂÜđĂîĕàöŤ
ēðøêĊ
đîÿÿĎ
Üÿč
éđöČę
ĂöĊ
Öćøđêĉ
ö Brij 35
øš
Ă÷úą 0.2 ĔîÿćøÿÖĆ
é éĆ
ÜîĆĚ
îÿćøúąúć÷ēàđéĊ
÷öôĂÿđôêïĆ
ôđôĂøŤ
ìĊę
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ïÙüćöđך
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î 50 öĉ
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ēöúćøŤ
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đĂß 7 ìĊę
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ēàđéĊ
÷öÙúĂĕøéŤ
ìĊę
øąéĆ
ïÙüćöđך
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îǰ1.25ǰēöúćøŤ
Ēúą Brij 35 øš
Ă÷úą 0.2 Ýċ
ÜđðŨ
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éìĊę
éĊ
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ÿč
éĔîÖćøđÖĘ
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÷üđĂîĕàöŤ
ēðø
êĊ
đîÿÝćÖöš
ćöðúćìĎ
îŠ
ćóĆ
îíčŤ
ÙøĊ
ï÷ćü
ÙĞ
ćÿĞ
ćÙĆ
â :
ēðøêĊ
đîÿ ðúćìĎ
îŠ
ćǰÖćøÿÖĆ
éǰÖćøđÖĘ
ïđÖĊę
÷üǰđÙøČę
ĂÜĔî
Abstract
The effect of extraction media on the recovery of proteinases from albacore tuna spleen
(
5IVOOVTǰBMBMVOHB
) was investigated. Types of extractants, NaCl concentration and types of surfactants
affected the proteinases extraction. The splenic extract using 50 mM sodium phosphate buffer, pH 7.0
showed higher proteolytic activity than those extracted with distilled water and 50 mM Tris-HCl, pH 7.0
(p<0.05). 50 mM sodium phosphate buffer, pH 7.0 containing different NaCl concentrations were used
to extract the proteinases from albacore tuna spleen. When NaCl concentration was increased (0-1.25
M), the proteolytic activity increased (p<0.05). The splenic extract showed the highest proteolytic
activity when isolated with 1.25 M NaCl. For effect of some surfactants, the highest yield of proteinases
extracted with the aid of 0.2% Brij 35 were obtained. Hence, 50 mM sodium phosphate buffer, pH 7.0
containing 1.25 M NaCl and 0.2% (v/v) Brij 35 was chosen as the extraction medium for proteinases
from albacore tuna spleen.
Keywords :
Proteinase, Tuna, Extraction, Recovery, Viscera
1
îĉ
ÿĉ
êðøĉ
ââćđĂÖ ĀúĆ
ÖÿĎ
êøđìÙēîēú÷Ċ
ßĊ
üõćó ÙèąđìÙēîēú÷Ċ
ĒúąÖćøóĆ
çîćßč
ößî öĀćüĉ
ì÷ćúĆ
÷ìĆ
Öþĉ
è óĆ
ìúč
Ü 93210
2
øý.éø., ÿć×ćüĉ
ßćüĉ
ì÷ćýćÿêøŤ
ĒúąđìÙēîēú÷Ċ
ĂćĀćø ÙèąđìÙēîēú÷Ċ
ĒúąÖćøóĆ
çîćßč
ößî öĀćüĉ
ì÷ćúĆ
÷ìĆ
Öþĉ
è óĆ
ìúč
Ü 93210
* Corresponding author :ēìøýĆ
óìŤ
/ēìøÿćø : 074-693996 E-mail address :
