911
P32
1
ùìíĉĝ
ÖŠ
ĂÖúć÷óĆ
îíčŤ
×ĂÜÿćøĂĂÖùìíĉĝ
ìćÜßĊ
üõćóÝćÖđßČĚ
ĂÿđêøðēêöĆ
÷àĊ
ìĒúąïøĊ
üĉ
ïćàĉ
úúĆ
ÿ
Mutagenicity Activity of Bioactive Compounds Produced from
4USFQUPNZDFT
spp. and
#SFWJCBDJMMVT
spp.
Öĉ
Ýêĉ
ýĆ
Öéĉĝ
ǰßüüĉ
ÿĉ
å
1,3
ǰǰèĆ
åíĉ
éćǰéĞ
ćîćéĊ
2
ǰǰîćëîĉ
íĉ
ǰđìĊę
÷Üíøøö
2
ǰǰîĉ
ÿćøĆ
êîŤ
ǰùìíĉ
øĆ
ÖþŤ
2
ǰĒúąöèæúǰđúĉ
ýÙèćüîĉ
ßÖč
ú
3,4*
Kittisak Chawawisit
1,3
Nattida Damnadee
2
, Natnithi Thiengtham
2
, Nisarat Rittirak
2
and
Monthon Lertcanawanichakul
3,4*
ïìÙĆ
é÷Š
Ă
ǰǰǰǰǰǰǰǰǰǰǰǰǰǰǰǰ
ǰîĞ
ćÿćøĂĂÖùìíĉ
ĝ
ìćÜßĊ
üõćóĔîîĚĞ
ćđúĊĚ
÷ÜđßČĚ
ĂÿđêøðēêöĆ
÷àĊ
ìĒúąïøĊ
üĉ
ïćàĉ
úúĆ
ÿöćýċ
Öþćùìíĉĝ
ÖŠ
ĂÖúć÷óĆ
îíčŤ
éš
ü÷üĉ
íĊ
đĂöÿŤ
ǰđöČę
ĂìéúĂÜĔîÿõćüąìĊę
öĊ
Öćøđêĉ
öĒúąĕöŠ
đêĉ
öđĂîĕàöŤ
ǰS9 ÝćÖêĆ
ïöîč
þ÷Ť
ǰēé÷Ĕßš
ĒïÙìĊ
đøĊ
÷àĆ
úēöđîúúćĕìôŗ
öĉ
üđøĊ
÷öǰÿć÷
óĆ
îíčŤ
ǰìĊ
đĂ98 ĒúąǰìĊ
đĂǰ100ǰđðŨ
îđßČĚ
ĂìéÿĂïǰĔîÖćøìĞ
ćĔĀš
đÖĉ
éÖćøÖúć÷óĆ
îíčŤ
ÖúĆ
ïöćđÝøĉ
âĕéš
ĔîÝćîđóćąđßČĚ
ĂìĊę
ĕöŠ
öĊ
ăĉ
ÿìĉ
éĊ
îǰ
ēé÷öĊ
đÖèæŤ
üŠ
ćĀćÖÝĞ
ćîüîēÙēúîĊ
Öúć÷óĆ
îíčŤ
ĔîÝćîìéÿĂïöĊ
ÙŠ
ćđìŠ
ćÖĆ
ïĀøČ
ĂöćÖÖüŠ
ćǰ 4 đìŠ
ćǰ×ĂÜēÙēúîĊ
ìĊę
đÖĉ
é×ċĚ
îđĂÜêćö
íøøößćêĉ
ÝćÖÝćîÙüïÙč
öñúúïǰĒÿéÜüŠ
ćÿćøĂĂÖùìíĉĝ
ìćÜßĊ
üõćóéĆ
ÜÖúŠ
ćüöĊ
ùìíĉĝ
ÖŠ
ĂÖúć÷óĆ
îíčŤ
ǰñúÖćøìéÿĂïóïüŠ
ćÿćø
ĂĂÖùìíĉĝ
ìćÜßĊ
üõćóĔîîĚĞ
ćđúĊĚ
÷ÜđßČĚ
ĂǰÿđêøðēêöĆ
÷àĊ
ìĒúąïøĊ
üĉ
ïćàĉ
úúĆ
ÿĕöŠ
öĊ
ùìíĉĝ
ÖŠ
ĂÖúć÷óĆ
îíčŤ
đöČę
ĂìéÿĂïéš
ü÷üĉ
íĊ
đĂöÿŤ
ìĆĚ
ÜìĊę
öĊ
Öćøđêĉ
öđĂîĕàöŤ
ĒúąĕöŠ
đêĉ
öđĂîĕàöŤ
ǰS9 ÝćÖêĆ
ïöîč
þ÷Ť
ǰǰǰ
ÙĞ
ćÿĞ
ćÙĆ
â:
ÿđêøðēêöĆ
÷àĊ
ìǰïøĊ
üĉ
ïćàĉ
úúĆ
ÿǰùìíĉĝ
ÖŠ
ĂÖúć÷óĆ
îíčŤ
ǰÖćøìéÿĂïđĂöÿŤ
Abstract
To investigate the mutagenicity activity of bioactive compounds in the culture broth of
4USFQUPNZDFTǰ
spp. and
#SFWJCBDJMMVT
spp. by means of Ames’ test in the condition with / without liver
enzyme S9. The revertant colonies of
4BMNPOFMMBǰUZQIJNVSJVN
TA98 and TA100 in the plate without
histidine were calculated compared with spontaneous colonies for the cause of mutagenicity in the
mutagenicity index >4 (MI>4). It was found that all of bioactive compound in culture broth from
4USFQUPNZDFT
spp. and
#SFWJCBDJMMVT
spp. did not show the mutagenicity activity in both strains of
4BMNPOFMMBǰ
when tested by Ames’ test in the condition with or without liver enzyme, S9.
Keywords:
4USFQUPNZDFTǰ
spp.,
#SFWJCBDJMMVTǰ
spp., Mutagenicity Activity, Ames’ Test
1
îĆ
Öýċ
Öþćðøĉ
ââćđĂÖǰĀúĆ
ÖÿĎ
êøßĊ
üđüßýćÿêøŤ
ǰÿĞ
ćîĆ
Öüĉ
ßćÿĀđüßýćÿêøŤ
Ēúąÿćíćøèÿč
×ýćÿêøŤ
ǰöĀćüĉ
ì÷ćúĆ
÷üúĆ
÷úĆ
ÖþèŤ
ǰîÙøýøĊ
íøøöøćßǰǰ
80161
2
ĀúĆ
ÖÿĎ
êøđìÙîĉ
ÙÖćøĒóì÷Ť
ǰÿĞ
ćîĆ
Öüĉ
ßćÿĀđüßýćÿêøŤ
Ēúąÿćíćøèÿč
×ýćÿêøŤ
ǰöĀćüĉ
ì÷ćúĆ
÷üúĆ
÷úĆ
ÖþèŤ
ǰîÙøýøĊ
íøøöøćßǰǰ80161
3
ĀîŠ
ü÷üĉ
ÝĆ
÷ÖćøĔßš
ðøąē÷ßîŤ
ñúĉ
êõĆ
èæŤ
íøøößćêĉ
ÿĞ
ćîĆ
Öüĉ
ßćüĉ
ì÷ćýćÿêøŤ
öĀćüĉ
ì÷ćúĆ
÷üúĆ
÷úĆ
ÖþèŤ
îÙøýøĊ
íøøöøćß 80161
4
øĂÜýćÿêøćÝćø÷Ť
ĀúĆ
ÖÿĎ
êøđìÙîĉ
ÙÖćøĒóì÷Ť
ÿĞ
ćîĆ
Öüĉ
ßćÿĀđüßýćÿêøŤ
Ēúąÿćíćøèÿč
×ýćÿêøŤ
öĀćüĉ
ì÷ćúĆ
÷üúĆ
÷úĆ
ÖþèŤ
îÙøýøĊ
íøøöøćß
80161
Corresponding author : Tel.: 075-672104 ext. 2180. E-mail address:
